A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio
Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium’s efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2017
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| Acceso en línea: | https://hdl.handle.net/10568/165030 |
| _version_ | 1855529197742587904 |
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| author | Rizal, Govinda Karki, Shanta Thakur, Vivek Wanchana, Samart Alonso-Cantabrana, Hugo Dionora, Jacque Sheehy, John E. Furbank, Robert von Caemmerer, Susanne Quick, William Paul |
| author_browse | Alonso-Cantabrana, Hugo Dionora, Jacque Furbank, Robert Karki, Shanta Quick, William Paul Rizal, Govinda Sheehy, John E. Thakur, Vivek Wanchana, Samart von Caemmerer, Susanne |
| author_facet | Rizal, Govinda Karki, Shanta Thakur, Vivek Wanchana, Samart Alonso-Cantabrana, Hugo Dionora, Jacque Sheehy, John E. Furbank, Robert von Caemmerer, Susanne Quick, William Paul |
| author_sort | Rizal, Govinda |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium’s efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum populations were generated and screened to identify genes that cause a loss of C4 function. Stable carbon isotope ratio (δ13C) of leaf dry matter has been used to distinguishspecies with C3 and C4 photosynthetic pathways. Here, we report the identification of a sorghum (Sorghum bicolor) mutant with a low δ13C characteristic. A mutant (named Mut33) with a pale phenotype and stunted growth was identified from an EMS treated sorghum M2 population. The stable carbon isotope analysis of the mutants showed a decrease of 13C uptake capacity. The noise of random mutation was reduced by crossing the mutant and its wildtype (WT). The back-cross (BC1F1) progenies were like the WT parent in terms of 13C values and plant phenotypes. All the BC1F2 plants with low δ13C died before they produced their 6th leaf. Gas exchange measurements of the low δ13C sorghum mutants showed a higher CO2 compensation point (25.24 μmol CO2.mol-1air) and the maximum rate of photosynthesis was less than 5μmol.m-2.s-1. To identify the genetic determinant of this trait, four DNA pools were isolated; two each from normal and low δ13C BC1F2 mutant plants. These were sequenced using an Illumina platform. Comparison of allele frequency of the single nucleotide polymorphisms (SNPs) between the pools with contrasting phenotype showed that a locus in Chromosome 10 between 57,941,104 and 59,985,708 bps had an allele frequency of 1. There were 211 mutations and 37 genes in the locus, out of which mutations in 9 genes showed non-synonymous changes. This finding is expected to contribute to future research on the identification of the causal factor differentiating C4 from C3 species that can be used in the transformation of C3 to C4 plants |
| format | Journal Article |
| id | CGSpace165030 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1650302025-01-24T14:13:17Z A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio Rizal, Govinda Karki, Shanta Thakur, Vivek Wanchana, Samart Alonso-Cantabrana, Hugo Dionora, Jacque Sheehy, John E. Furbank, Robert von Caemmerer, Susanne Quick, William Paul Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium’s efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum populations were generated and screened to identify genes that cause a loss of C4 function. Stable carbon isotope ratio (δ13C) of leaf dry matter has been used to distinguishspecies with C3 and C4 photosynthetic pathways. Here, we report the identification of a sorghum (Sorghum bicolor) mutant with a low δ13C characteristic. A mutant (named Mut33) with a pale phenotype and stunted growth was identified from an EMS treated sorghum M2 population. The stable carbon isotope analysis of the mutants showed a decrease of 13C uptake capacity. The noise of random mutation was reduced by crossing the mutant and its wildtype (WT). The back-cross (BC1F1) progenies were like the WT parent in terms of 13C values and plant phenotypes. All the BC1F2 plants with low δ13C died before they produced their 6th leaf. Gas exchange measurements of the low δ13C sorghum mutants showed a higher CO2 compensation point (25.24 μmol CO2.mol-1air) and the maximum rate of photosynthesis was less than 5μmol.m-2.s-1. To identify the genetic determinant of this trait, four DNA pools were isolated; two each from normal and low δ13C BC1F2 mutant plants. These were sequenced using an Illumina platform. Comparison of allele frequency of the single nucleotide polymorphisms (SNPs) between the pools with contrasting phenotype showed that a locus in Chromosome 10 between 57,941,104 and 59,985,708 bps had an allele frequency of 1. There were 211 mutations and 37 genes in the locus, out of which mutations in 9 genes showed non-synonymous changes. This finding is expected to contribute to future research on the identification of the causal factor differentiating C4 from C3 species that can be used in the transformation of C3 to C4 plants 2017-06-22 2024-12-19T12:54:37Z 2024-12-19T12:54:37Z Journal Article https://hdl.handle.net/10568/165030 en Open Access Public Library of Science Rizal, Govinda; Karki, Shanta; Thakur, Vivek; Wanchana, Samart; Alonso-Cantabrana, Hugo; Dionora, Jacque; Sheehy, John E.; Furbank, Robert; von Caemmerer, Susanne and Quick, William Paul. 2017. A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio. PLoS ONE, Volume 12 no. 6 p. e0179567 |
| spellingShingle | Rizal, Govinda Karki, Shanta Thakur, Vivek Wanchana, Samart Alonso-Cantabrana, Hugo Dionora, Jacque Sheehy, John E. Furbank, Robert von Caemmerer, Susanne Quick, William Paul A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title | A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title_full | A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title_fullStr | A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title_full_unstemmed | A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title_short | A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio |
| title_sort | sorghum sorghum bicolor mutant with altered carbon isotope ratio |
| url | https://hdl.handle.net/10568/165030 |
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