Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley
Terminal drought stress during grain filling is the major abiotic factor that limits crop yield in barley. The mother plant acclimates to the environment and perceives signals that result in a change of the physiological state within the grain and therefore affect the seed development and germinatio...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2017
|
| Acceso en línea: | https://hdl.handle.net/10568/165027 |
| _version_ | 1855539874611855360 |
|---|---|
| author | Surdonja, Korana Eggert, Kai Hajirezaei, Mohammad-Reza Harshavardhan, Vokkaliga Seiler, Christiane Von Wirén, Nicolaus Sreenivasulu, Nese Kuhlmann, Markus |
| author_browse | Eggert, Kai Hajirezaei, Mohammad-Reza Harshavardhan, Vokkaliga Kuhlmann, Markus Seiler, Christiane Sreenivasulu, Nese Surdonja, Korana Von Wirén, Nicolaus |
| author_facet | Surdonja, Korana Eggert, Kai Hajirezaei, Mohammad-Reza Harshavardhan, Vokkaliga Seiler, Christiane Von Wirén, Nicolaus Sreenivasulu, Nese Kuhlmann, Markus |
| author_sort | Surdonja, Korana |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Terminal drought stress during grain filling is the major abiotic factor that limits crop yield in barley. The mother plant acclimates to the environment and perceives signals that result in a change of the physiological state within the grain and therefore affect the seed development and germination of the next generation. Small regulatory RNAs have been described to be involved in plant drought stress response by suppressing the respective target genes. Based on their origin and function, these small RNAs are classified as micro RNAs (miRNA), short interfering RNAs (siRNA) or heterochromatic small interfering RNA (hc-siRNA). In addition, 24mer sized hc-siRNAs are associated with RNA directed DNA methylation (RdDM) and transcriptional gene silencing (TGS). The analysis of hc-siRNA by small RNA sequencing in barley caryopses after imposition of terminal drought stress allowed the identification of stress specific 24mers. Based on the sequence homology of the siRNAs to the promoter region of CYTOKININ-OXIDASE 2.1 (HvCKX2.1), this putative target gene was selected for further investigation. Terminal drought stress leads to an increased level of DNA methylation at the HvCKX2.1 promoter and the seeds derived from drought stressed plants showed faster shoot emergence. Accumulation of cytokinin ribosides, which are the known substrates of cytokinin-oxidase, can explain the observed phenotype of faster shoot emergence from seeds of drought stressed mother plants. Analysis of transgenic plants with modulated levels of abscisic acid (ABA) in the grain confirmed the ABA/drought stress responsive ProHvCKX2.1 methylation and correlation with shoot emergence speed. |
| format | Journal Article |
| id | CGSpace165027 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1650272025-12-08T09:54:28Z Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley Surdonja, Korana Eggert, Kai Hajirezaei, Mohammad-Reza Harshavardhan, Vokkaliga Seiler, Christiane Von Wirén, Nicolaus Sreenivasulu, Nese Kuhlmann, Markus Terminal drought stress during grain filling is the major abiotic factor that limits crop yield in barley. The mother plant acclimates to the environment and perceives signals that result in a change of the physiological state within the grain and therefore affect the seed development and germination of the next generation. Small regulatory RNAs have been described to be involved in plant drought stress response by suppressing the respective target genes. Based on their origin and function, these small RNAs are classified as micro RNAs (miRNA), short interfering RNAs (siRNA) or heterochromatic small interfering RNA (hc-siRNA). In addition, 24mer sized hc-siRNAs are associated with RNA directed DNA methylation (RdDM) and transcriptional gene silencing (TGS). The analysis of hc-siRNA by small RNA sequencing in barley caryopses after imposition of terminal drought stress allowed the identification of stress specific 24mers. Based on the sequence homology of the siRNAs to the promoter region of CYTOKININ-OXIDASE 2.1 (HvCKX2.1), this putative target gene was selected for further investigation. Terminal drought stress leads to an increased level of DNA methylation at the HvCKX2.1 promoter and the seeds derived from drought stressed plants showed faster shoot emergence. Accumulation of cytokinin ribosides, which are the known substrates of cytokinin-oxidase, can explain the observed phenotype of faster shoot emergence from seeds of drought stressed mother plants. Analysis of transgenic plants with modulated levels of abscisic acid (ABA) in the grain confirmed the ABA/drought stress responsive ProHvCKX2.1 methylation and correlation with shoot emergence speed. 2017-06-27 2024-12-19T12:54:37Z 2024-12-19T12:54:37Z Journal Article https://hdl.handle.net/10568/165027 en Open Access MDPI Surdonja, Korana; Eggert, Kai; Hajirezaei, Mohammad-Reza; Harshavardhan, Vokkaliga; Seiler, Christiane; Von Wirén, Nicolaus; Sreenivasulu, Nese and Kuhlmann, Markus. 2017. Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley. Epigenomes, Volume 1 no. 2 p. 9 |
| spellingShingle | Surdonja, Korana Eggert, Kai Hajirezaei, Mohammad-Reza Harshavardhan, Vokkaliga Seiler, Christiane Von Wirén, Nicolaus Sreenivasulu, Nese Kuhlmann, Markus Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title | Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title_full | Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title_fullStr | Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title_full_unstemmed | Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title_short | Increase of DNA Methylation at the HvCKX2.1 promoter by terminal drought stress in barley |
| title_sort | increase of dna methylation at the hvckx2 1 promoter by terminal drought stress in barley |
| url | https://hdl.handle.net/10568/165027 |
| work_keys_str_mv | AT surdonjakorana increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT eggertkai increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT hajirezaeimohammadreza increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT harshavardhanvokkaliga increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT seilerchristiane increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT vonwirennicolaus increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT sreenivasulunese increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley AT kuhlmannmarkus increaseofdnamethylationatthehvckx21promoterbyterminaldroughtstressinbarley |