Contrasting tocol ratios associated with seed longevity in rice variety groups

Vitamin E (tocols) is a key metabolite for efficient scavenging of lipid peroxy radicals that cause membrane breakdown during seed ageing. However, in rice, this hypothesis has been tested for very few lines only and without considering intraspecific variation in genomic structure. Here, we present...

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Main Authors: Lee, Jae-Sung, Kwak, Jieun, Yoon, Mi-Ra, Lee, Jeom-Sig, Hay, Fiona R.
Format: Journal Article
Language:Inglés
Published: Cambridge University Press 2017
Online Access:https://hdl.handle.net/10568/164981
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author Lee, Jae-Sung
Kwak, Jieun
Yoon, Mi-Ra
Lee, Jeom-Sig
Hay, Fiona R.
author_browse Hay, Fiona R.
Kwak, Jieun
Lee, Jae-Sung
Lee, Jeom-Sig
Yoon, Mi-Ra
author_facet Lee, Jae-Sung
Kwak, Jieun
Yoon, Mi-Ra
Lee, Jeom-Sig
Hay, Fiona R.
author_sort Lee, Jae-Sung
collection Repository of Agricultural Research Outputs (CGSpace)
description Vitamin E (tocols) is a key metabolite for efficient scavenging of lipid peroxy radicals that cause membrane breakdown during seed ageing. However, in rice, this hypothesis has been tested for very few lines only and without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing Aus, Basmati/Sadri, Indica, temperate Japonica and tropical Japonica variety groups, were used for tocols analysis (quantification of α-, β-, γ- and δ-tocopherol/tocotrienol by ultra-performance liquid chromatography) and storage experiments at 45°C and 10.9% seed moisture content. To examine the effects of DNA-haplotype on the phenotype, the 700 K high-density single-nucleotide polymorphism marker dataset was utilized. Both seed longevity (time for viability to fall to 50%; p50) and tocols content varied across variety groups related to the heterogeneity in the genetic architecture. Among eight types of tocol homologues, α-tocopherol and γ-tocotrienol were significantly correlated with p50 (negatively and positively, respectively). While temperate Japonica varieties were most abundant in α-tocopherol, Indica varieties recorded 1.3- to 1.7-fold higher γ-tocotrienol than those of other groups. We conclude that the specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.
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spelling CGSpace1649812025-01-23T08:27:27Z Contrasting tocol ratios associated with seed longevity in rice variety groups Lee, Jae-Sung Kwak, Jieun Yoon, Mi-Ra Lee, Jeom-Sig Hay, Fiona R. Vitamin E (tocols) is a key metabolite for efficient scavenging of lipid peroxy radicals that cause membrane breakdown during seed ageing. However, in rice, this hypothesis has been tested for very few lines only and without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing Aus, Basmati/Sadri, Indica, temperate Japonica and tropical Japonica variety groups, were used for tocols analysis (quantification of α-, β-, γ- and δ-tocopherol/tocotrienol by ultra-performance liquid chromatography) and storage experiments at 45°C and 10.9% seed moisture content. To examine the effects of DNA-haplotype on the phenotype, the 700 K high-density single-nucleotide polymorphism marker dataset was utilized. Both seed longevity (time for viability to fall to 50%; p50) and tocols content varied across variety groups related to the heterogeneity in the genetic architecture. Among eight types of tocol homologues, α-tocopherol and γ-tocotrienol were significantly correlated with p50 (negatively and positively, respectively). While temperate Japonica varieties were most abundant in α-tocopherol, Indica varieties recorded 1.3- to 1.7-fold higher γ-tocotrienol than those of other groups. We conclude that the specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism. 2017-12 2024-12-19T12:54:35Z 2024-12-19T12:54:35Z Journal Article https://hdl.handle.net/10568/164981 en Cambridge University Press Lee, Jae-Sung; Kwak, Jieun; Yoon, Mi-Ra; Lee, Jeom-Sig and Hay, Fiona R. 2017. Genetic loci governing grain yield and root development under variable rice cultivation conditions. Seed Sci. Res., Volume 27 no. 4 p. 273-280
spellingShingle Lee, Jae-Sung
Kwak, Jieun
Yoon, Mi-Ra
Lee, Jeom-Sig
Hay, Fiona R.
Contrasting tocol ratios associated with seed longevity in rice variety groups
title Contrasting tocol ratios associated with seed longevity in rice variety groups
title_full Contrasting tocol ratios associated with seed longevity in rice variety groups
title_fullStr Contrasting tocol ratios associated with seed longevity in rice variety groups
title_full_unstemmed Contrasting tocol ratios associated with seed longevity in rice variety groups
title_short Contrasting tocol ratios associated with seed longevity in rice variety groups
title_sort contrasting tocol ratios associated with seed longevity in rice variety groups
url https://hdl.handle.net/10568/164981
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