High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points
To assist with efforts to engineer a C4 photosynthetic pathway into rice, forward-genetic approaches are being used to identify the genes modulating key C4 traits. Currently, a major challenge is how to screen for a variety of different traits in a high-throughput manner. Here we describe a method f...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Commonwealth Scientific and Industrial Research Organisation
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164925 |
| _version_ | 1855530550540894208 |
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| author | Coe, Robert A. Chatterjee, Jolly Acebron, Kelvin Dionora, Jacqueline Mogul, Reychelle Lin, HsiangChun Yin, Xiaojia Bandyopadhyay, Anindya Sirault, Xavier R. R. Furbank, Robert T. Quick, W. Paul |
| author_browse | Acebron, Kelvin Bandyopadhyay, Anindya Chatterjee, Jolly Coe, Robert A. Dionora, Jacqueline Furbank, Robert T. Lin, HsiangChun Mogul, Reychelle Quick, W. Paul Sirault, Xavier R. R. Yin, Xiaojia |
| author_facet | Coe, Robert A. Chatterjee, Jolly Acebron, Kelvin Dionora, Jacqueline Mogul, Reychelle Lin, HsiangChun Yin, Xiaojia Bandyopadhyay, Anindya Sirault, Xavier R. R. Furbank, Robert T. Quick, W. Paul |
| author_sort | Coe, Robert A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | To assist with efforts to engineer a C4 photosynthetic pathway into rice, forward-genetic approaches are being used to identify the genes modulating key C4 traits. Currently, a major challenge is how to screen for a variety of different traits in a high-throughput manner. Here we describe a method for identifying C4 mutant plants with increased CO2 compensation points. This is used as a signature for decreased photosynthetic efficiency associated with a loss of C4 function. By exposing plants to a CO2 concentration close to the CO2 compensation point of a wild-type plant, individuals can be identified from measurements of chlorophyll a fluorescence. We use this method to screen a mutant population of the C4 monocot Setaria viridis (L.)P.Beauv. generated using N-nitroso-N-methylurea (NMU). Mutants were identified at a frequency of 1 per 157 lines screened. Forty-six candidate lines were identified and one line with a heritable homozygous phenotype selected for further characterisation. The CO2 compensation point of this mutant was increased to a value similar to that of C3 rice. Photosynthesis and growth was significantly reduced under ambient conditions. These data indicate that the screen was capable of identifying mutants with decreased photosynthetic efficiency. Characterisation and next-generation sequencing of all the mutants identified in this screen may lead to the discovery of novel genes underpinning C4 photosynthesis. These can be used to engineer a C4 photosynthetic pathway into rice. |
| format | Journal Article |
| id | CGSpace164925 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Commonwealth Scientific and Industrial Research Organisation |
| publisherStr | Commonwealth Scientific and Industrial Research Organisation |
| record_format | dspace |
| spelling | CGSpace1649252025-12-08T10:29:22Z High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points Coe, Robert A. Chatterjee, Jolly Acebron, Kelvin Dionora, Jacqueline Mogul, Reychelle Lin, HsiangChun Yin, Xiaojia Bandyopadhyay, Anindya Sirault, Xavier R. R. Furbank, Robert T. Quick, W. Paul chlorophyll fluorescence forward genetics mutants photosynthesis setaria viridis To assist with efforts to engineer a C4 photosynthetic pathway into rice, forward-genetic approaches are being used to identify the genes modulating key C4 traits. Currently, a major challenge is how to screen for a variety of different traits in a high-throughput manner. Here we describe a method for identifying C4 mutant plants with increased CO2 compensation points. This is used as a signature for decreased photosynthetic efficiency associated with a loss of C4 function. By exposing plants to a CO2 concentration close to the CO2 compensation point of a wild-type plant, individuals can be identified from measurements of chlorophyll a fluorescence. We use this method to screen a mutant population of the C4 monocot Setaria viridis (L.)P.Beauv. generated using N-nitroso-N-methylurea (NMU). Mutants were identified at a frequency of 1 per 157 lines screened. Forty-six candidate lines were identified and one line with a heritable homozygous phenotype selected for further characterisation. The CO2 compensation point of this mutant was increased to a value similar to that of C3 rice. Photosynthesis and growth was significantly reduced under ambient conditions. These data indicate that the screen was capable of identifying mutants with decreased photosynthetic efficiency. Characterisation and next-generation sequencing of all the mutants identified in this screen may lead to the discovery of novel genes underpinning C4 photosynthesis. These can be used to engineer a C4 photosynthetic pathway into rice. 2018 2024-12-19T12:54:28Z 2024-12-19T12:54:28Z Journal Article https://hdl.handle.net/10568/164925 en Commonwealth Scientific and Industrial Research Organisation Coe, Robert A.; Chatterjee, Jolly; Acebron, Kelvin; Dionora, Jacqueline; Mogul, Reychelle; Lin, HsiangChun; Yin, Xiaojia; Bandyopadhyay, Anindya; Sirault, Xavier R. R.; Furbank, Robert T. and Quick, W. Paul. 2018. High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points. Functional Plant Biol., Volume 45 no. 10 p. 1017 |
| spellingShingle | chlorophyll fluorescence forward genetics mutants photosynthesis setaria viridis Coe, Robert A. Chatterjee, Jolly Acebron, Kelvin Dionora, Jacqueline Mogul, Reychelle Lin, HsiangChun Yin, Xiaojia Bandyopadhyay, Anindya Sirault, Xavier R. R. Furbank, Robert T. Quick, W. Paul High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title | High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title_full | High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title_fullStr | High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title_full_unstemmed | High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title_short | High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points |
| title_sort | high throughput chlorophyll fluorescence screening of setaria viridis for mutants with altered co2 compensation points |
| topic | chlorophyll fluorescence forward genetics mutants photosynthesis setaria viridis |
| url | https://hdl.handle.net/10568/164925 |
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