Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses
Proliferation of plasmodesmata (PD) connections between bundle sheath (BS) and mesophyll (M) cells has been proposed as a key step in the evolution of two-cell C4 photosynthesis; However, a lack of quantitative data has hampered further exploration and validation of this hypothesis. In this study, w...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2018
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164918 |
| _version_ | 1855538306188574720 |
|---|---|
| author | Danila, Florence R. Quick, William Paul White, Rosemary G. Kelly, Steven von Caemmerer, Susanne Furbank, Robert T. |
| author_browse | Danila, Florence R. Furbank, Robert T. Kelly, Steven Quick, William Paul White, Rosemary G. von Caemmerer, Susanne |
| author_facet | Danila, Florence R. Quick, William Paul White, Rosemary G. Kelly, Steven von Caemmerer, Susanne Furbank, Robert T. |
| author_sort | Danila, Florence R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Proliferation of plasmodesmata (PD) connections between bundle sheath (BS) and mesophyll (M) cells has been proposed as a key step in the evolution of two-cell C4 photosynthesis; However, a lack of quantitative data has hampered further exploration and validation of this hypothesis. In this study, we quantified leaf anatomical traits associated with metabolite transport in 18 species of BEP and PACMAD grasses encompassing four origins of C4 photosynthesis and all three C4 subtypes (NADP-ME, NAD-ME, and PCK). We demonstrate that C4 leaves have greater PD density between M and BS cells than C3 leaves. We show that this greater PD density is achieved by increasing either the pit field (cluster of PD) area or the number of PD per pit field area. NAD-ME species had greater pit field area per M-BS interface than NADP-ME or PCK species. In contrast, NADP-ME and PCK species had lower pit field area with increased number of PD per pit field area than NAD-ME species. Overall, PD density per M-BS cell interface was greatest in NAD-ME species while PD density in PCK species exhibited the largest variability. Finally, the only other anatomical characteristic that clearly distinguished C4 from C3 species was their greater Sb value, the BS surface area to subtending leaf area ratio. In contrast, BS cell volume was comparable between the C3 and C4 grass species examined. |
| format | Journal Article |
| id | CGSpace164918 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1649182025-12-08T09:54:28Z Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses Danila, Florence R. Quick, William Paul White, Rosemary G. Kelly, Steven von Caemmerer, Susanne Furbank, Robert T. mesophyll metabolites photosynthesis weeds Proliferation of plasmodesmata (PD) connections between bundle sheath (BS) and mesophyll (M) cells has been proposed as a key step in the evolution of two-cell C4 photosynthesis; However, a lack of quantitative data has hampered further exploration and validation of this hypothesis. In this study, we quantified leaf anatomical traits associated with metabolite transport in 18 species of BEP and PACMAD grasses encompassing four origins of C4 photosynthesis and all three C4 subtypes (NADP-ME, NAD-ME, and PCK). We demonstrate that C4 leaves have greater PD density between M and BS cells than C3 leaves. We show that this greater PD density is achieved by increasing either the pit field (cluster of PD) area or the number of PD per pit field area. NAD-ME species had greater pit field area per M-BS interface than NADP-ME or PCK species. In contrast, NADP-ME and PCK species had lower pit field area with increased number of PD per pit field area than NAD-ME species. Overall, PD density per M-BS cell interface was greatest in NAD-ME species while PD density in PCK species exhibited the largest variability. Finally, the only other anatomical characteristic that clearly distinguished C4 from C3 species was their greater Sb value, the BS surface area to subtending leaf area ratio. In contrast, BS cell volume was comparable between the C3 and C4 grass species examined. 2018-02-23 2024-12-19T12:54:28Z 2024-12-19T12:54:28Z Journal Article https://hdl.handle.net/10568/164918 en Open Access Oxford University Press Danila, Florence R; Quick, William Paul; White, Rosemary G; Kelly, Steven; von Caemmerer, Susanne and Furbank, Robert T. 2018. Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses. Journal of Experimental Botany, volume 69, no. 5; pages 1135-1145, ill. Ref. |
| spellingShingle | mesophyll metabolites photosynthesis weeds Danila, Florence R. Quick, William Paul White, Rosemary G. Kelly, Steven von Caemmerer, Susanne Furbank, Robert T. Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title | Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title_full | Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title_fullStr | Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title_full_unstemmed | Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title_short | Multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of C-4 grasses |
| title_sort | multiple mechanisms for enhanced plasmodesmata density in disparate subtypes of c 4 grasses |
| topic | mesophyll metabolites photosynthesis weeds |
| url | https://hdl.handle.net/10568/164918 |
| work_keys_str_mv | AT danilaflorencer multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses AT quickwilliampaul multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses AT whiterosemaryg multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses AT kellysteven multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses AT voncaemmerersusanne multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses AT furbankrobertt multiplemechanismsforenhancedplasmodesmatadensityindisparatesubtypesofc4grasses |