Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice
Drought and low nitrogen are the most common abiotic stresses limiting rice productivity in the rainfed rice areas of Asia and Africa. Development and adoption of green super rice (GSR) varieties with greatly improved drought tolerance (DT) and low nitrogen tolerance (LNT) are the most efficient way...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164899 |
| _version_ | 1855537614671577088 |
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| author | Feng, Bo Chen, Kai Cui, Yanru Wu, Zhichao Zheng, Tianqing Zhu, Yajun Ali, Jauhar Wang, Bingbing Xu, Jianlong Zhang, Wenzhong Li, Zhikang |
| author_browse | Ali, Jauhar Chen, Kai Cui, Yanru Feng, Bo Li, Zhikang Wang, Bingbing Wu, Zhichao Xu, Jianlong Zhang, Wenzhong Zheng, Tianqing Zhu, Yajun |
| author_facet | Feng, Bo Chen, Kai Cui, Yanru Wu, Zhichao Zheng, Tianqing Zhu, Yajun Ali, Jauhar Wang, Bingbing Xu, Jianlong Zhang, Wenzhong Li, Zhikang |
| author_sort | Feng, Bo |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought and low nitrogen are the most common abiotic stresses limiting rice productivity in the rainfed rice areas of Asia and Africa. Development and adoption of green super rice (GSR) varieties with greatly improved drought tolerance (DT) and low nitrogen tolerance (LNT) are the most efficient way to resolve this problem. In this study, using three sets of trait-specific introgression lines (ILs) in a Xian (indica) variety Huanghuazhan (HHZ) background, we identified nine DT-QTL and seven LNT QTL by a segregation distortion approach and a genome-wide association study, respectively. Based on performances of DT and LNT and genotypes at the detected QTL, two ILs M79 and M387 with DT and LNT were selected for cross-making to validate the identified QTL and to develop DT and LNT rice lines by pyramiding two DT-QTL (qDT3.9 and qDT6.3) and two LNT-QTL (qGY1 and qSF8). Using four pairs of kompetitive allele specific PCR (KASP) SNP markers, we selected 66 F2 individuals with different combinations of the target DT- and LNT-QTL favorable alleles and they showed expected improvement in DT and/or LNT, which were further validated by the significant improvement in DT and/or LNT of their F3 progeny testing. Based on evaluation of pyramiding lines in F3 lines under drought, low nitrogen (LN) and normal conditions, four promising pyramiding lines having different QTL favorable alleles were selected, which showed significantly improved tolerances to drought and/or LN than HHZ and their IL parents. Our results demonstrated that trait-specific ILs could effectively connect QTL mapping and QTL pyramiding breeding, and designed QTL pyramiding (DQP) using ILs could be more effective in molecular rice breeding for complex quantitative traits |
| format | Journal Article |
| id | CGSpace164899 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1648992024-12-19T14:13:27Z Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice Feng, Bo Chen, Kai Cui, Yanru Wu, Zhichao Zheng, Tianqing Zhu, Yajun Ali, Jauhar Wang, Bingbing Xu, Jianlong Zhang, Wenzhong Li, Zhikang breeding drought gene pyramiding nitrogen plant breeding quantitative trait loci Drought and low nitrogen are the most common abiotic stresses limiting rice productivity in the rainfed rice areas of Asia and Africa. Development and adoption of green super rice (GSR) varieties with greatly improved drought tolerance (DT) and low nitrogen tolerance (LNT) are the most efficient way to resolve this problem. In this study, using three sets of trait-specific introgression lines (ILs) in a Xian (indica) variety Huanghuazhan (HHZ) background, we identified nine DT-QTL and seven LNT QTL by a segregation distortion approach and a genome-wide association study, respectively. Based on performances of DT and LNT and genotypes at the detected QTL, two ILs M79 and M387 with DT and LNT were selected for cross-making to validate the identified QTL and to develop DT and LNT rice lines by pyramiding two DT-QTL (qDT3.9 and qDT6.3) and two LNT-QTL (qGY1 and qSF8). Using four pairs of kompetitive allele specific PCR (KASP) SNP markers, we selected 66 F2 individuals with different combinations of the target DT- and LNT-QTL favorable alleles and they showed expected improvement in DT and/or LNT, which were further validated by the significant improvement in DT and/or LNT of their F3 progeny testing. Based on evaluation of pyramiding lines in F3 lines under drought, low nitrogen (LN) and normal conditions, four promising pyramiding lines having different QTL favorable alleles were selected, which showed significantly improved tolerances to drought and/or LN than HHZ and their IL parents. Our results demonstrated that trait-specific ILs could effectively connect QTL mapping and QTL pyramiding breeding, and designed QTL pyramiding (DQP) using ILs could be more effective in molecular rice breeding for complex quantitative traits 2018-03-09 2024-12-19T12:54:27Z 2024-12-19T12:54:27Z Journal Article https://hdl.handle.net/10568/164899 en Open Access Frontiers Media Feng, Bo; Chen, Kai; Cui, Yanru; Wu, Zhichao; Zheng, Tianqing; Zhu, Yajun; Ali, Jauhar; Wang, Bingbing; Xu, Jianlong; Zhang, Wenzhong and Li, Zhikang. 2018. Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice. Front. Plant Sci., Volume 9 |
| spellingShingle | breeding drought gene pyramiding nitrogen plant breeding quantitative trait loci Feng, Bo Chen, Kai Cui, Yanru Wu, Zhichao Zheng, Tianqing Zhu, Yajun Ali, Jauhar Wang, Bingbing Xu, Jianlong Zhang, Wenzhong Li, Zhikang Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title | Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title_full | Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title_fullStr | Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title_full_unstemmed | Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title_short | Genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed QTL pyramiding in rice |
| title_sort | genetic dissection and simultaneous improvement of drought and low nitrogen tolerances by designed qtl pyramiding in rice |
| topic | breeding drought gene pyramiding nitrogen plant breeding quantitative trait loci |
| url | https://hdl.handle.net/10568/164899 |
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