Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus
Rice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164882 |
| _version_ | 1855540387705257984 |
|---|---|
| author | Macovei, Anca Sevilla, Neah R. Cantos, Christian Jonson, Gilda B. Slamet-Loedin, Inez Čermák, Tomáš Voytas, Daniel F. Choi, Il-Ryong Chadha-Mohanty, Prabhjit |
| author_browse | Cantos, Christian Chadha-Mohanty, Prabhjit Choi, Il-Ryong Jonson, Gilda B. Macovei, Anca Sevilla, Neah R. Slamet-Loedin, Inez Voytas, Daniel F. Čermák, Tomáš |
| author_facet | Macovei, Anca Sevilla, Neah R. Cantos, Christian Jonson, Gilda B. Slamet-Loedin, Inez Čermák, Tomáš Voytas, Daniel F. Choi, Il-Ryong Chadha-Mohanty, Prabhjit |
| author_sort | Macovei, Anca |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of RTD in the field. Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). The Y1059V1060V1061 residues of eIF4G are known to be associated with the reactions to RTSV. To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV‐susceptible variety IR64, widely grown across tropical Asia. The mutation rates ranged from 36.0% to 86.6%, depending on the target site, and the mutations were successfully transmitted to the next generations. Among various mutated eIF4G alleles examined, only those resulting in in‐frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance. Furthermore, our data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eIF4G could not be maintained in homozygous state. The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties. |
| format | Journal Article |
| id | CGSpace164882 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1648822025-05-14T10:24:29Z Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus Macovei, Anca Sevilla, Neah R. Cantos, Christian Jonson, Gilda B. Slamet-Loedin, Inez Čermák, Tomáš Voytas, Daniel F. Choi, Il-Ryong Chadha-Mohanty, Prabhjit alleles disease resistance mutations plant viruses resistance genes rice tungro virus Rice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of RTD in the field. Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). The Y1059V1060V1061 residues of eIF4G are known to be associated with the reactions to RTSV. To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV‐susceptible variety IR64, widely grown across tropical Asia. The mutation rates ranged from 36.0% to 86.6%, depending on the target site, and the mutations were successfully transmitted to the next generations. Among various mutated eIF4G alleles examined, only those resulting in in‐frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance. Furthermore, our data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eIF4G could not be maintained in homozygous state. The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties. 2018-11 2024-12-19T12:54:26Z 2024-12-19T12:54:26Z Journal Article https://hdl.handle.net/10568/164882 en Open Access Wiley Macovei, Anca; Sevilla, Neah R.; Cantos, Christian; Jonson, Gilda B.; Slamet‐Loedin, Inez; Čermák, Tomáš; Voytas, Daniel F.; Choi, Il‐Ryong and Chadha‐Mohanty, Prabhjit. 2018. Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus. Plant Biotechnology Journal, Volume 16 no. 11 p. 1918-1927 |
| spellingShingle | alleles disease resistance mutations plant viruses resistance genes rice tungro virus Macovei, Anca Sevilla, Neah R. Cantos, Christian Jonson, Gilda B. Slamet-Loedin, Inez Čermák, Tomáš Voytas, Daniel F. Choi, Il-Ryong Chadha-Mohanty, Prabhjit Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title | Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title_full | Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title_fullStr | Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title_full_unstemmed | Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title_short | Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus |
| title_sort | novel alleles of rice eif4g generated by crispr cas9 targeted mutagenesis confer resistance to rice tungro spherical virus |
| topic | alleles disease resistance mutations plant viruses resistance genes rice tungro virus |
| url | https://hdl.handle.net/10568/164882 |
| work_keys_str_mv | AT macoveianca novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT sevillaneahr novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT cantoschristian novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT jonsongildab novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT slametloedininez novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT cermaktomas novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT voytasdanielf novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT choiilryong novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus AT chadhamohantyprabhjit novelallelesofriceeif4ggeneratedbycrisprcas9targetedmutagenesisconferresistancetoricetungrosphericalvirus |