Abscisic acid influences tillering by modulation of strigolactones in barley
Strigolactones (SLs) represent a class of plant hormones that are involved in inhibiting shoot branching and in promoting abiotic stress responses. There is evidence that the biosynthetic pathways of SLs and abscisic acid (ABA) are functionally connected. However, little is known about the mechanism...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164840 |
| _version_ | 1855524813787889664 |
|---|---|
| author | Wang, Hongwen Chen, Wanxin Eggert, Kai Charnikhova, Tatsiana Bouwmeester, Harro Schweizer, Patrick Hajirezaei, Mohammad R. Seiler, Christiane Sreenivasulu, Nese von Wirén, Nicolaus Kuhlmann, Markus |
| author_browse | Bouwmeester, Harro Charnikhova, Tatsiana Chen, Wanxin Eggert, Kai Hajirezaei, Mohammad R. Kuhlmann, Markus Schweizer, Patrick Seiler, Christiane Sreenivasulu, Nese Wang, Hongwen von Wirén, Nicolaus |
| author_facet | Wang, Hongwen Chen, Wanxin Eggert, Kai Charnikhova, Tatsiana Bouwmeester, Harro Schweizer, Patrick Hajirezaei, Mohammad R. Seiler, Christiane Sreenivasulu, Nese von Wirén, Nicolaus Kuhlmann, Markus |
| author_sort | Wang, Hongwen |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Strigolactones (SLs) represent a class of plant hormones that are involved in inhibiting shoot branching and in promoting abiotic stress responses. There is evidence that the biosynthetic pathways of SLs and abscisic acid (ABA) are functionally connected. However, little is known about the mechanisms underlying the interaction of SLs and ABA, and the relevance of this interaction for shoot architecture. Based on sequence homology, four genes (HvD27, HvMAX1, HvCCD7, and HvCCD8) involved in SL biosynthesis were identified in barley and functionally verified by complementation of Arabidopsis mutants or by virus-induced gene silencing. To investigate the influence of ABA on SLs, two transgenic lines accumulating ABA as a result of RNAi-mediated down-regulation of HvABA 8’-hydroxylase 1 and 3 were employed. LC-MS/MS analysis confirmed higher ABA levels in root and stem base tissues in these transgenic lines. Both lines showed enhanced tiller formation and lower concentrations of 5-deoxystrigol in root exudates, which was detected for the first time as a naturally occurring SL in barley. Lower expression levels of HvD27, HvMAX1, HvCCD7, and HvCCD8 indicated that ABA suppresses SL biosynthesis, leading to enhanced tiller formation in barley. |
| format | Journal Article |
| id | CGSpace164840 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1648402025-12-08T09:54:28Z Abscisic acid influences tillering by modulation of strigolactones in barley Wang, Hongwen Chen, Wanxin Eggert, Kai Charnikhova, Tatsiana Bouwmeester, Harro Schweizer, Patrick Hajirezaei, Mohammad R. Seiler, Christiane Sreenivasulu, Nese von Wirén, Nicolaus Kuhlmann, Markus abscisic acid biosynthesis environmental factors gene expression genetic transformation phytochemicals plant growth substances root exudates stress tillering transgenics Strigolactones (SLs) represent a class of plant hormones that are involved in inhibiting shoot branching and in promoting abiotic stress responses. There is evidence that the biosynthetic pathways of SLs and abscisic acid (ABA) are functionally connected. However, little is known about the mechanisms underlying the interaction of SLs and ABA, and the relevance of this interaction for shoot architecture. Based on sequence homology, four genes (HvD27, HvMAX1, HvCCD7, and HvCCD8) involved in SL biosynthesis were identified in barley and functionally verified by complementation of Arabidopsis mutants or by virus-induced gene silencing. To investigate the influence of ABA on SLs, two transgenic lines accumulating ABA as a result of RNAi-mediated down-regulation of HvABA 8’-hydroxylase 1 and 3 were employed. LC-MS/MS analysis confirmed higher ABA levels in root and stem base tissues in these transgenic lines. Both lines showed enhanced tiller formation and lower concentrations of 5-deoxystrigol in root exudates, which was detected for the first time as a naturally occurring SL in barley. Lower expression levels of HvD27, HvMAX1, HvCCD7, and HvCCD8 indicated that ABA suppresses SL biosynthesis, leading to enhanced tiller formation in barley. 2018-07-18 2024-12-19T12:54:21Z 2024-12-19T12:54:21Z Journal Article https://hdl.handle.net/10568/164840 en Open Access Oxford University Press Wang, H., Chen, W., Eggert, K., Charnikhova, T., Bouwmeester, H., Schweizer, P., Hajirezaei, M. R., Seiler, C., Sreenivasulu, N., von Wirén, N., & Kuhlmann, M. (2018). Abscisic acid influences tillering by modulation of strigolactones in barley. Journal of Experimental Botany, 69(16), 3883-3898. https://doi.org/10.1093/jxb/ery200 |
| spellingShingle | abscisic acid biosynthesis environmental factors gene expression genetic transformation phytochemicals plant growth substances root exudates stress tillering transgenics Wang, Hongwen Chen, Wanxin Eggert, Kai Charnikhova, Tatsiana Bouwmeester, Harro Schweizer, Patrick Hajirezaei, Mohammad R. Seiler, Christiane Sreenivasulu, Nese von Wirén, Nicolaus Kuhlmann, Markus Abscisic acid influences tillering by modulation of strigolactones in barley |
| title | Abscisic acid influences tillering by modulation of strigolactones in barley |
| title_full | Abscisic acid influences tillering by modulation of strigolactones in barley |
| title_fullStr | Abscisic acid influences tillering by modulation of strigolactones in barley |
| title_full_unstemmed | Abscisic acid influences tillering by modulation of strigolactones in barley |
| title_short | Abscisic acid influences tillering by modulation of strigolactones in barley |
| title_sort | abscisic acid influences tillering by modulation of strigolactones in barley |
| topic | abscisic acid biosynthesis environmental factors gene expression genetic transformation phytochemicals plant growth substances root exudates stress tillering transgenics |
| url | https://hdl.handle.net/10568/164840 |
| work_keys_str_mv | AT wanghongwen abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT chenwanxin abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT eggertkai abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT charnikhovatatsiana abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT bouwmeesterharro abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT schweizerpatrick abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT hajirezaeimohammadr abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT seilerchristiane abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT sreenivasulunese abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT vonwirennicolaus abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley AT kuhlmannmarkus abscisicacidinfluencestilleringbymodulationofstrigolactonesinbarley |