Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene
The degree of heterosis in different hybrid rice varieties is reported to be at the highest in indica/japonica cross combination, however, there is a problem of sterility and semi-sterility in such inter sub specific hybrids. To overcome this problem, it is essential to develop parental lines having...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164833 |
| _version_ | 1855542233764200448 |
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| author | Priyadarshi, Rahul Arremsetty, Hari P. S. Singh, Akhilesh K. Khandekar, Durga Ulaganathan, Kandasamy Shenoy, Vinay Sinha, Pallavi Singh, Vikas K. |
| author_browse | Arremsetty, Hari P. S. Khandekar, Durga Priyadarshi, Rahul Shenoy, Vinay Singh, Akhilesh K. Singh, Vikas K. Sinha, Pallavi Ulaganathan, Kandasamy |
| author_facet | Priyadarshi, Rahul Arremsetty, Hari P. S. Singh, Akhilesh K. Khandekar, Durga Ulaganathan, Kandasamy Shenoy, Vinay Sinha, Pallavi Singh, Vikas K. |
| author_sort | Priyadarshi, Rahul |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The degree of heterosis in different hybrid rice varieties is reported to be at the highest in indica/japonica cross combination, however, there is a problem of sterility and semi-sterility in such inter sub specific hybrids. To overcome this problem, it is essential to develop parental lines having wide compatibility (S5(n)) gene. In this study, a functional marker S5-InDel was used for marker-assisted backcrossing (MABB) to introgress S5(n) gene from Dular into the genetic background of a widely grown recurrent parent IR 58025B, a maintainer line of wild-abortive (WA) cytoplasmic male sterile line, IR 58025A. Further, a closely linked marker nksbadh2 was used for the identification of plants devoid of aroma in backcross population to develop hybrids with no aroma. The stringent phenotypic selection followed by background selection of BC3F4 identified plants with 94.51-98.90% of the recurrent parent genome recovery of lines carrying S5(n) gene. Subsequently, at 10 promising BC3F5 lines possessing S5(n) gene with high yielding and long-slender grain type were validated for their maintainer behavior through test crosses with IR 58025A. Also the improved lines showed significantly improved spikelet fertility performance while crossed with japonica and javanica testers in comparison to the original recurrent parent. The improved lines developed in the present study, are being converted to CMS lines through marker-assisted backcross breeding to facilitate precise and improved hybrid breeding program in rice. |
| format | Journal Article |
| id | CGSpace164833 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1648332024-12-19T14:13:45Z Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene Priyadarshi, Rahul Arremsetty, Hari P. S. Singh, Akhilesh K. Khandekar, Durga Ulaganathan, Kandasamy Shenoy, Vinay Sinha, Pallavi Singh, Vikas K. backcrossing breeding programmes genetic markers genetic parameters genomes heterosis phenotypes plant breeding spikelets varieties yields The degree of heterosis in different hybrid rice varieties is reported to be at the highest in indica/japonica cross combination, however, there is a problem of sterility and semi-sterility in such inter sub specific hybrids. To overcome this problem, it is essential to develop parental lines having wide compatibility (S5(n)) gene. In this study, a functional marker S5-InDel was used for marker-assisted backcrossing (MABB) to introgress S5(n) gene from Dular into the genetic background of a widely grown recurrent parent IR 58025B, a maintainer line of wild-abortive (WA) cytoplasmic male sterile line, IR 58025A. Further, a closely linked marker nksbadh2 was used for the identification of plants devoid of aroma in backcross population to develop hybrids with no aroma. The stringent phenotypic selection followed by background selection of BC3F4 identified plants with 94.51-98.90% of the recurrent parent genome recovery of lines carrying S5(n) gene. Subsequently, at 10 promising BC3F5 lines possessing S5(n) gene with high yielding and long-slender grain type were validated for their maintainer behavior through test crosses with IR 58025A. Also the improved lines showed significantly improved spikelet fertility performance while crossed with japonica and javanica testers in comparison to the original recurrent parent. The improved lines developed in the present study, are being converted to CMS lines through marker-assisted backcross breeding to facilitate precise and improved hybrid breeding program in rice. 2018-07-20 2024-12-19T12:54:21Z 2024-12-19T12:54:21Z Journal Article https://hdl.handle.net/10568/164833 en Open Access Frontiers Media Priyadarshi, Rahul; Arremsetty, Hari P. S.; Singh, Akhilesh K.; Khandekar, Durga; Ulaganathan, Kandasamy; Shenoy, Vinay; Sinha, Pallavi and Singh, Vikas K. 2018. Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene. Front. Plant Sci., Volume 9 |
| spellingShingle | backcrossing breeding programmes genetic markers genetic parameters genomes heterosis phenotypes plant breeding spikelets varieties yields Priyadarshi, Rahul Arremsetty, Hari P. S. Singh, Akhilesh K. Khandekar, Durga Ulaganathan, Kandasamy Shenoy, Vinay Sinha, Pallavi Singh, Vikas K. Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title | Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title_full | Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title_fullStr | Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title_full_unstemmed | Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title_short | Marker-assisted improvement of the elite maintainer line of rice, IR 58025B for wide compatibility (S5(n)) gene |
| title_sort | marker assisted improvement of the elite maintainer line of rice ir 58025b for wide compatibility s5 n gene |
| topic | backcrossing breeding programmes genetic markers genetic parameters genomes heterosis phenotypes plant breeding spikelets varieties yields |
| url | https://hdl.handle.net/10568/164833 |
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