Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition
Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164817 |
| _version_ | 1855543267122216960 |
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| author | Luo, Bingbing Chen, Jingguang Zhu, Longlong Liu, Shuhua Li, Bin Lu, Hong Ye, Guoyou Xu, Guohua Fan, Xiaorong |
| author_browse | Chen, Jingguang Fan, Xiaorong Li, Bin Liu, Shuhua Lu, Hong Luo, Bingbing Xu, Guohua Ye, Guoyou Zhu, Longlong |
| author_facet | Luo, Bingbing Chen, Jingguang Zhu, Longlong Liu, Shuhua Li, Bin Lu, Hong Ye, Guoyou Xu, Guohua Fan, Xiaorong |
| author_sort | Luo, Bingbing |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing OsNRT2.1 and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% reduction in grain yields of wild type (WT) plants compared to WL condition. Interestingly, the overexpression of OsNRT2.1 could recover this loss, as OsNRT2.1 transgenic lines displayed higher grain yields than WT plants. We also observed 60% higher grain Mn in the transgenic lines in AWD condition and approximately 30% higher Mn in the grain of transgenic lines in WL condition. We further found that the overexpression of OsNRT2.1 did not alter Mg and Fe in the seeds in either growth condition. The reasons for the increased Mn content in OsNRT2.1 transgenic seeds in AWD condition could be explained by the elevated expression of OsNRAMP family genes including OsNRAMP3, OsNRAMP5, and OsNRAMP6 in node I, the panicle-neck, and the flag leaves. The mechanism(s) underpinning the upregulation of these genes requires further investigation. Taken together, our results provide a new function of OsNRT2.1 in improving rice yields and grain Mn accumulation during water-saving cultivation patterns. This represents a new strategy for maintaining yield and improving food quality in a sustainable agricultural system. |
| format | Journal Article |
| id | CGSpace164817 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1648172024-12-19T14:13:49Z Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition Luo, Bingbing Chen, Jingguang Zhu, Longlong Liu, Shuhua Li, Bin Lu, Hong Ye, Guoyou Xu, Guohua Fan, Xiaorong alternate wetting and drying genetic expression manganese nitrates transporters yields Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing OsNRT2.1 and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% reduction in grain yields of wild type (WT) plants compared to WL condition. Interestingly, the overexpression of OsNRT2.1 could recover this loss, as OsNRT2.1 transgenic lines displayed higher grain yields than WT plants. We also observed 60% higher grain Mn in the transgenic lines in AWD condition and approximately 30% higher Mn in the grain of transgenic lines in WL condition. We further found that the overexpression of OsNRT2.1 did not alter Mg and Fe in the seeds in either growth condition. The reasons for the increased Mn content in OsNRT2.1 transgenic seeds in AWD condition could be explained by the elevated expression of OsNRAMP family genes including OsNRAMP3, OsNRAMP5, and OsNRAMP6 in node I, the panicle-neck, and the flag leaves. The mechanism(s) underpinning the upregulation of these genes requires further investigation. Taken together, our results provide a new function of OsNRT2.1 in improving rice yields and grain Mn accumulation during water-saving cultivation patterns. This represents a new strategy for maintaining yield and improving food quality in a sustainable agricultural system. 2018-08-15 2024-12-19T12:54:20Z 2024-12-19T12:54:20Z Journal Article https://hdl.handle.net/10568/164817 en Open Access Frontiers Media Luo, Bingbing; Chen, Jingguang; Zhu, Longlong; Liu, Shuhua; Li, Bin; Lu, Hong; Ye, Guoyou; Xu, Guohua and Fan, Xiaorong. 2018. Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition. Front. Plant Sci., Volume 9 |
| spellingShingle | alternate wetting and drying genetic expression manganese nitrates transporters yields Luo, Bingbing Chen, Jingguang Zhu, Longlong Liu, Shuhua Li, Bin Lu, Hong Ye, Guoyou Xu, Guohua Fan, Xiaorong Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title | Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title_full | Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title_fullStr | Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title_full_unstemmed | Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title_short | Overexpression of a high-affinity nitrate transporter OsNRT2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| title_sort | overexpression of a high affinity nitrate transporter osnrt2 1 increases yield and manganese accumulation in rice under alternating wet and dry condition |
| topic | alternate wetting and drying genetic expression manganese nitrates transporters yields |
| url | https://hdl.handle.net/10568/164817 |
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