Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the major diseases that impact rice production in Asia. The bacteria use transcription activator-like effectors (TALEs) to hijack the host transcription machinery and activate key susceptibility (S) genes, specifically member...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164805 |
| _version_ | 1855539320270618624 |
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| author | Zaka, Abha Grande, Genelou Coronejo, Thea Quibod, Ian Lorenzo Chen, Chun-Wei Chang, Su-Jein Szurek, Boris Arif, Muhammad Vera Cruz, Casiana Oliva, Ricardo |
| author_browse | Arif, Muhammad Chang, Su-Jein Chen, Chun-Wei Coronejo, Thea Grande, Genelou Oliva, Ricardo Quibod, Ian Lorenzo Szurek, Boris Vera Cruz, Casiana Zaka, Abha |
| author_facet | Zaka, Abha Grande, Genelou Coronejo, Thea Quibod, Ian Lorenzo Chen, Chun-Wei Chang, Su-Jein Szurek, Boris Arif, Muhammad Vera Cruz, Casiana Oliva, Ricardo |
| author_sort | Zaka, Abha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the major diseases that impact rice production in Asia. The bacteria use transcription activator-like effectors (TALEs) to hijack the host transcription machinery and activate key susceptibility (S) genes, specifically members of the SWEET sucrose uniporters through the recognition of effector-binding element (EBEs) in the promoter regions. However, natural variations in the EBEs that alter the binding affinity of TALEs usually prevent sufficient induction of SWEET genes, leading to resistance phenotypes. In this study, we identified candidate resistance alleles by mining a rice diversity panel for mutations in the promoter of OsSWEET13 and OsSWEET14, which are direct targets of three major TALEs PthXo2, PthXo3 and AvrXa7. We found natural variations at the EBE of both genes, which appeared to have emerged independently in at least three rice subspecies. For OsSWEET13, a 2-bp deletion at the 5th and 6th positions of the EBE, and a substitution at the 17th position appear to be sufficient to prevent activation by PthXo2. Similarly, a single nucleotide substitution at position 10 compromised the induction of OsSWEET14 by AvrXa7. These findings might increase our opportunities to reduce pathogen virulence by preventing the induction of SWEET transporters. Pyramiding variants along with other resistance genes may provide durable and broad-spectrum resistance to the disease. |
| format | Journal Article |
| id | CGSpace164805 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1648052025-05-14T10:24:28Z Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae Zaka, Abha Grande, Genelou Coronejo, Thea Quibod, Ian Lorenzo Chen, Chun-Wei Chang, Su-Jein Szurek, Boris Arif, Muhammad Vera Cruz, Casiana Oliva, Ricardo alleles bacterial diseases bacterial diseases disease resistance pathogenicity plant pathogenic bacteria xanthomonas oryzae pv oryzae Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the major diseases that impact rice production in Asia. The bacteria use transcription activator-like effectors (TALEs) to hijack the host transcription machinery and activate key susceptibility (S) genes, specifically members of the SWEET sucrose uniporters through the recognition of effector-binding element (EBEs) in the promoter regions. However, natural variations in the EBEs that alter the binding affinity of TALEs usually prevent sufficient induction of SWEET genes, leading to resistance phenotypes. In this study, we identified candidate resistance alleles by mining a rice diversity panel for mutations in the promoter of OsSWEET13 and OsSWEET14, which are direct targets of three major TALEs PthXo2, PthXo3 and AvrXa7. We found natural variations at the EBE of both genes, which appeared to have emerged independently in at least three rice subspecies. For OsSWEET13, a 2-bp deletion at the 5th and 6th positions of the EBE, and a substitution at the 17th position appear to be sufficient to prevent activation by PthXo2. Similarly, a single nucleotide substitution at position 10 compromised the induction of OsSWEET14 by AvrXa7. These findings might increase our opportunities to reduce pathogen virulence by preventing the induction of SWEET transporters. Pyramiding variants along with other resistance genes may provide durable and broad-spectrum resistance to the disease. 2018-09-13 2024-12-19T12:54:19Z 2024-12-19T12:54:19Z Journal Article https://hdl.handle.net/10568/164805 en Open Access Public Library of Science Zaka, Abha; Grande, Genelou; Coronejo, Thea; Quibod, Ian Lorenzo; Chen, Chun-Wei; Chang, Su-Jein; Szurek, Boris; Arif, Muhammad; Cruz, Casiana Vera and Oliva, Ricardo. 2018. Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae. PLoS ONE, Volume 13 no. 9 p. e0203711 |
| spellingShingle | alleles bacterial diseases bacterial diseases disease resistance pathogenicity plant pathogenic bacteria xanthomonas oryzae pv oryzae Zaka, Abha Grande, Genelou Coronejo, Thea Quibod, Ian Lorenzo Chen, Chun-Wei Chang, Su-Jein Szurek, Boris Arif, Muhammad Vera Cruz, Casiana Oliva, Ricardo Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title | Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title_full | Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title_fullStr | Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title_short | Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae |
| title_sort | natural variations in the promoter of ossweet13 and ossweet14 expand the range of resistance against xanthomonas oryzae pv oryzae |
| topic | alleles bacterial diseases bacterial diseases disease resistance pathogenicity plant pathogenic bacteria xanthomonas oryzae pv oryzae |
| url | https://hdl.handle.net/10568/164805 |
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