Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery
Chronic allograft dysfunction (CAD), the foremost cause of renal graft loss worldwide, is a serious challenge for most of the recipients. As the epigenetic era is emerging, epigenetic biomarkers especially microRNAs (miRNAs) may reflect the current stage of the disease and patient’s therapy response...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Acceso en línea: | https://hdl.handle.net/10568/164786 |
| _version_ | 1855542696211382272 |
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| author | Nariman-Saleh-Fam, Ziba Bastami, Milad Ardalan, Mohammadreza Sharifi, Simin Hosseinian Khatib, Seyed Mahdi Zununi Vahed, Sepideh |
| author_browse | Ardalan, Mohammadreza Bastami, Milad Hosseinian Khatib, Seyed Mahdi Nariman-Saleh-Fam, Ziba Sharifi, Simin Zununi Vahed, Sepideh |
| author_facet | Nariman-Saleh-Fam, Ziba Bastami, Milad Ardalan, Mohammadreza Sharifi, Simin Hosseinian Khatib, Seyed Mahdi Zununi Vahed, Sepideh |
| author_sort | Nariman-Saleh-Fam, Ziba |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Chronic allograft dysfunction (CAD), the foremost cause of renal graft loss worldwide, is a serious challenge for most of the recipients. As the epigenetic era is emerging, epigenetic biomarkers especially microRNAs (miRNAs) may reflect the current stage of the disease and patient’s therapy response. The current study investigated the potential significance of circulating miRNA‐148a in predicting the renal graft function.Circulating miRNAs were isolated from 53 plasma samples of recipients with histologically validated interstitial fibrosis and tubular atrophy (IFTA, n = 26), and recipients with stable graft function (SGF, n = 27), and also healthy individuals ( n = 15). The level of miRNA‐148a was evaluated by the quantitative polymerase chain reaction (qPCR) and correlated with clinical and histological parameters.Significantly, miRNA‐148a decreased in IFTA compared with SGF subjects (P < 0.001). MiRNA‐148a levels indicated a significant association with serum creatinine levels ( r = 0.451, P = 0.021) and glomerular filtration rate ( r = −0.520, P = 0.006). MiRNA‐148a expression levels could discriminate IFTA cases from SGF individuals with an area under the curve of 0.89 ( P < 0.001), 97% sensitivity, and 72% specificity. A number of predicted targets that might be involved in CAD by miRNA‐148a were predicted.Plasma cell‐free miRNA‐148a correlated with renal function and histological grades; therefore, it may be further investigated as a novel noninvasive molecular marker of the progression to IFTA in renal transplant recipients; moreover, the emerging biomarker may become a therapeutic target in the future clinic. |
| format | Journal Article |
| id | CGSpace164786 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1647862025-05-14T10:24:33Z Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery Nariman-Saleh-Fam, Ziba Bastami, Milad Ardalan, Mohammadreza Sharifi, Simin Hosseinian Khatib, Seyed Mahdi Zununi Vahed, Sepideh health human diseases human nutrition Chronic allograft dysfunction (CAD), the foremost cause of renal graft loss worldwide, is a serious challenge for most of the recipients. As the epigenetic era is emerging, epigenetic biomarkers especially microRNAs (miRNAs) may reflect the current stage of the disease and patient’s therapy response. The current study investigated the potential significance of circulating miRNA‐148a in predicting the renal graft function.Circulating miRNAs were isolated from 53 plasma samples of recipients with histologically validated interstitial fibrosis and tubular atrophy (IFTA, n = 26), and recipients with stable graft function (SGF, n = 27), and also healthy individuals ( n = 15). The level of miRNA‐148a was evaluated by the quantitative polymerase chain reaction (qPCR) and correlated with clinical and histological parameters.Significantly, miRNA‐148a decreased in IFTA compared with SGF subjects (P < 0.001). MiRNA‐148a levels indicated a significant association with serum creatinine levels ( r = 0.451, P = 0.021) and glomerular filtration rate ( r = −0.520, P = 0.006). MiRNA‐148a expression levels could discriminate IFTA cases from SGF individuals with an area under the curve of 0.89 ( P < 0.001), 97% sensitivity, and 72% specificity. A number of predicted targets that might be involved in CAD by miRNA‐148a were predicted.Plasma cell‐free miRNA‐148a correlated with renal function and histological grades; therefore, it may be further investigated as a novel noninvasive molecular marker of the progression to IFTA in renal transplant recipients; moreover, the emerging biomarker may become a therapeutic target in the future clinic. 2019-04 2024-12-19T12:54:19Z 2024-12-19T12:54:19Z Journal Article https://hdl.handle.net/10568/164786 en Wiley Nariman‐Saleh‐Fam, Ziba; Bastami, Milad; Ardalan, Mohammadreza; Sharifi, Simin; Hosseinian Khatib, Seyed Mahdi and Zununi Vahed, Sepideh. 2018. Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery. J of Cellular Biochemistry, Volume 120 no. 4 p. 5737-5746 |
| spellingShingle | health human diseases human nutrition Nariman-Saleh-Fam, Ziba Bastami, Milad Ardalan, Mohammadreza Sharifi, Simin Hosseinian Khatib, Seyed Mahdi Zununi Vahed, Sepideh Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title | Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title_full | Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title_fullStr | Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title_full_unstemmed | Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title_short | Cell‐free microRNA‐148a is associated with renal allograft dysfunction: Implication for biomarker discovery |
| title_sort | cell free microrna 148a is associated with renal allograft dysfunction implication for biomarker discovery |
| topic | health human diseases human nutrition |
| url | https://hdl.handle.net/10568/164786 |
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