Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance
Pyramiding resistance genes is predicted to increase the durability of resistant rice varieties against phloem-feeding herbivores. We examined responses by the green leafhopper, Nephotettix virescens (Hemiptera: Cicadellidae), to near-isogenic rice lines with zero, one and two resistance genes. The...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164777 |
| _version_ | 1855537102534475776 |
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| author | Horgan, Finbarr G. Bernal, Carmencita C. Vu, Quynh Almazan, Maria Liberty P. Ramal, Angelee Fame Yasui, Hideshi Fujita, Daisuke |
| author_browse | Almazan, Maria Liberty P. Bernal, Carmencita C. Fujita, Daisuke Horgan, Finbarr G. Ramal, Angelee Fame Vu, Quynh Yasui, Hideshi |
| author_facet | Horgan, Finbarr G. Bernal, Carmencita C. Vu, Quynh Almazan, Maria Liberty P. Ramal, Angelee Fame Yasui, Hideshi Fujita, Daisuke |
| author_sort | Horgan, Finbarr G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pyramiding resistance genes is predicted to increase the durability of resistant rice varieties against phloem-feeding herbivores. We examined responses by the green leafhopper, Nephotettix virescens (Hemiptera: Cicadellidae), to near-isogenic rice lines with zero, one and two resistance genes. The recurrent parent (T65) and monogenic lines (GRH2-NIL and GRH4-NIL) with genes for resistance to the green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), were susceptible to the green leafhopper, but the pyramided line (GRH2/GRH4-PYL) was highly resistant to the green leafhopper. We selected green leafhoppers, N. virescens, from five sites in the Philippines for over 20 generations on each of the four lines. Populations selected on GRH2/GRH4-PYL gained partial virulence (feeding and development equal to that on T65) to the pyramided line within 10 generations and complete virulence (egg-laying equal to that on T65) within 20 generations. After 20 generations of rearing on the susceptible monogenic lines, green leafhoppers were also capable of developing and laying eggs on GRH2/GRH4-PYL. Furthermore, green leafhoppers reared on the susceptible GRH4-NIL for 20 generations showed equal preferences for T65 and GRH2/GRH4-PYL in choice bioassays. Our results indicate that previous long-term exposure to ineffective genes (including unperceived resistance genes) could dramatically reduce the durability of pyramided resistance. We suggest that informed crop management and deployment strategies should be developed to accompany rice lines with pyramided resistance and avoid the build-up of virulent herbivore populations. |
| format | Journal Article |
| id | CGSpace164777 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1647772024-12-19T14:13:25Z Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance Horgan, Finbarr G. Bernal, Carmencita C. Vu, Quynh Almazan, Maria Liberty P. Ramal, Angelee Fame Yasui, Hideshi Fujita, Daisuke bioassays crop management disease resistance genetic markers integrated pest management pathogenicity pesticide resistance plant breeding plant protection Pyramiding resistance genes is predicted to increase the durability of resistant rice varieties against phloem-feeding herbivores. We examined responses by the green leafhopper, Nephotettix virescens (Hemiptera: Cicadellidae), to near-isogenic rice lines with zero, one and two resistance genes. The recurrent parent (T65) and monogenic lines (GRH2-NIL and GRH4-NIL) with genes for resistance to the green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), were susceptible to the green leafhopper, but the pyramided line (GRH2/GRH4-PYL) was highly resistant to the green leafhopper. We selected green leafhoppers, N. virescens, from five sites in the Philippines for over 20 generations on each of the four lines. Populations selected on GRH2/GRH4-PYL gained partial virulence (feeding and development equal to that on T65) to the pyramided line within 10 generations and complete virulence (egg-laying equal to that on T65) within 20 generations. After 20 generations of rearing on the susceptible monogenic lines, green leafhoppers were also capable of developing and laying eggs on GRH2/GRH4-PYL. Furthermore, green leafhoppers reared on the susceptible GRH4-NIL for 20 generations showed equal preferences for T65 and GRH2/GRH4-PYL in choice bioassays. Our results indicate that previous long-term exposure to ineffective genes (including unperceived resistance genes) could dramatically reduce the durability of pyramided resistance. We suggest that informed crop management and deployment strategies should be developed to accompany rice lines with pyramided resistance and avoid the build-up of virulent herbivore populations. 2018-11 2024-12-19T12:54:18Z 2024-12-19T12:54:18Z Journal Article https://hdl.handle.net/10568/164777 en Open Access Elsevier Horgan, Finbarr G.; Bernal, Carmencita C.; Vu, Quynh; Almazan, Maria Liberty P.; Ramal, Angelee Fame; Yasui, Hideshi and Fujita, Daisuke. 2018. Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance. Crop Protection, Volume 113 p. 40-47 |
| spellingShingle | bioassays crop management disease resistance genetic markers integrated pest management pathogenicity pesticide resistance plant breeding plant protection Horgan, Finbarr G. Bernal, Carmencita C. Vu, Quynh Almazan, Maria Liberty P. Ramal, Angelee Fame Yasui, Hideshi Fujita, Daisuke Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title | Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title_full | Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title_fullStr | Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title_full_unstemmed | Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title_short | Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance |
| title_sort | virulence adaptation in a rice leafhopper exposure to ineffective genes compromises pyramided resistance |
| topic | bioassays crop management disease resistance genetic markers integrated pest management pathogenicity pesticide resistance plant breeding plant protection |
| url | https://hdl.handle.net/10568/164777 |
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