Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm
Heat stress occurrence during seed filling leads to the formation of a chalky portion in the limited zone of the starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene express...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2019
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164766 |
| _version_ | 1855536222695325696 |
|---|---|
| author | Ishimaru, Tsutomu Parween, Sabiha Saito, Yuhi Shigemitsu, Takanari Yamakawa, Hiromoto Nakazono, Mikio Masumura, Takehiro Nishizawa, Naoko K. Kondo, Motohiko Sreenivasulu, Nese |
| author_browse | Ishimaru, Tsutomu Kondo, Motohiko Masumura, Takehiro Nakazono, Mikio Nishizawa, Naoko K. Parween, Sabiha Saito, Yuhi Shigemitsu, Takanari Sreenivasulu, Nese Yamakawa, Hiromoto |
| author_facet | Ishimaru, Tsutomu Parween, Sabiha Saito, Yuhi Shigemitsu, Takanari Yamakawa, Hiromoto Nakazono, Mikio Masumura, Takehiro Nishizawa, Naoko K. Kondo, Motohiko Sreenivasulu, Nese |
| author_sort | Ishimaru, Tsutomu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Heat stress occurrence during seed filling leads to the formation of a chalky portion in the limited zone of the starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene expression analysis of a 44 K microarray was performed to identify key regulatory genes involved in the formation of milky-white (MW) and white-back (WB) grains during heat stress. Gene regulatory network analysis classified the genes changed under heat stress into five modules. The most distinct expression pattern was observed in modules where most of the small heat shock proteins and cellular organization genes were changed under heat stress in dorsal aleurone cells and dorsal starchy endosperm zones. The histological observation supported the significant increase in cell number and size of dorsal aleurone cells in WB grains. With regard to the central starchy endosperm zone, preferential down-regulation of high molecular weight heat shock proteins (HMW HSPs), including a prominent member encoding endoplasmic reticulum (ER) chaperones, by heat stress was observed, while changes in expression of starch biosynthesis genes were minimal. Characterization of transgenic plants suppressing endosperm lumenal binding protein gene (BiP1), an ER chaperone preferentially down-regulated at the MW zone under heat stress, showed evidence of forming the chalky grains without disturbing the expression of starch biosynthesis genes. The present LM-based comprehensive expression analysis provides novel inferences that HMW HSPs play an important role in controlling redox, nitrogen and amino acid metabolism in endosperm leading to the formation of MW and WB chalky grains under heat stress |
| format | Journal Article |
| id | CGSpace164766 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1647662025-05-14T10:24:23Z Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm Ishimaru, Tsutomu Parween, Sabiha Saito, Yuhi Shigemitsu, Takanari Yamakawa, Hiromoto Nakazono, Mikio Masumura, Takehiro Nishizawa, Naoko K. Kondo, Motohiko Sreenivasulu, Nese cell biology general medicine physiology plant science Heat stress occurrence during seed filling leads to the formation of a chalky portion in the limited zone of the starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene expression analysis of a 44 K microarray was performed to identify key regulatory genes involved in the formation of milky-white (MW) and white-back (WB) grains during heat stress. Gene regulatory network analysis classified the genes changed under heat stress into five modules. The most distinct expression pattern was observed in modules where most of the small heat shock proteins and cellular organization genes were changed under heat stress in dorsal aleurone cells and dorsal starchy endosperm zones. The histological observation supported the significant increase in cell number and size of dorsal aleurone cells in WB grains. With regard to the central starchy endosperm zone, preferential down-regulation of high molecular weight heat shock proteins (HMW HSPs), including a prominent member encoding endoplasmic reticulum (ER) chaperones, by heat stress was observed, while changes in expression of starch biosynthesis genes were minimal. Characterization of transgenic plants suppressing endosperm lumenal binding protein gene (BiP1), an ER chaperone preferentially down-regulated at the MW zone under heat stress, showed evidence of forming the chalky grains without disturbing the expression of starch biosynthesis genes. The present LM-based comprehensive expression analysis provides novel inferences that HMW HSPs play an important role in controlling redox, nitrogen and amino acid metabolism in endosperm leading to the formation of MW and WB chalky grains under heat stress 2019-03-01 2024-12-19T12:54:16Z 2024-12-19T12:54:16Z Journal Article https://hdl.handle.net/10568/164766 en Open Access Oxford University Press Ishimaru, Tsutomu; Parween, Sabiha; Saito, Yuhi; Shigemitsu, Takanari; Yamakawa, Hiromoto; Nakazono, Mikio; Masumura, Takehiro; Nishizawa, Naoko K; Kondo, Motohiko and Sreenivasulu, Nese. 2019. Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm. Plant and Cell Physiology, Volume 60 no. 3 p. 626-642 |
| spellingShingle | cell biology general medicine physiology plant science Ishimaru, Tsutomu Parween, Sabiha Saito, Yuhi Shigemitsu, Takanari Yamakawa, Hiromoto Nakazono, Mikio Masumura, Takehiro Nishizawa, Naoko K. Kondo, Motohiko Sreenivasulu, Nese Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title | Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title_full | Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title_fullStr | Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title_full_unstemmed | Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title_short | Laser Microdissection-Based Tissue-Specific Transcriptome Analysis Reveals a Novel Regulatory Network of Genes Involved in Heat-Induced Grain Chalk in Rice Endosperm |
| title_sort | laser microdissection based tissue specific transcriptome analysis reveals a novel regulatory network of genes involved in heat induced grain chalk in rice endosperm |
| topic | cell biology general medicine physiology plant science |
| url | https://hdl.handle.net/10568/164766 |
| work_keys_str_mv | AT ishimarutsutomu lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT parweensabiha lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT saitoyuhi lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT shigemitsutakanari lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT yamakawahiromoto lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT nakazonomikio lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT masumuratakehiro lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT nishizawanaokok lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT kondomotohiko lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm AT sreenivasulunese lasermicrodissectionbasedtissuespecifictranscriptomeanalysisrevealsanovelregulatorynetworkofgenesinvolvedinheatinducedgrainchalkinriceendosperm |