Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves
The influence of reduced glycine decarboxylase complex (GDC) activity on leaf atmosphere CO2 and (CO2)-C-13 exchange was tested in transgenic Oryza sativa with the GDC H-subunit knocked down in leaf mesophyll cells. Leaf measurements on transgenic gdch knockdown and wild-type plants were carried out...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2019
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| Online Access: | https://hdl.handle.net/10568/164716 |
| _version_ | 1855519759918956544 |
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| author | Giuliani, Rita Karki, Shanta Covshoff, Sarah Lin, Hsiang-Chun Coe, Robert A. Koteyeva, Nuria K. Quick, W. Paul von Caemmerer, Susanne Furbank, Robert T. Hibberd, Julian M. Edwards, Gerald E. Cousins, Asaph B. |
| author_browse | Coe, Robert A. Cousins, Asaph B. Covshoff, Sarah Edwards, Gerald E. Furbank, Robert T. Giuliani, Rita Hibberd, Julian M. Karki, Shanta Koteyeva, Nuria K. Lin, Hsiang-Chun Quick, W. Paul von Caemmerer, Susanne |
| author_facet | Giuliani, Rita Karki, Shanta Covshoff, Sarah Lin, Hsiang-Chun Coe, Robert A. Koteyeva, Nuria K. Quick, W. Paul von Caemmerer, Susanne Furbank, Robert T. Hibberd, Julian M. Edwards, Gerald E. Cousins, Asaph B. |
| author_sort | Giuliani, Rita |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The influence of reduced glycine decarboxylase complex (GDC) activity on leaf atmosphere CO2 and (CO2)-C-13 exchange was tested in transgenic Oryza sativa with the GDC H-subunit knocked down in leaf mesophyll cells. Leaf measurements on transgenic gdch knockdown and wild-type plants were carried out in the light under photorespiratory and low photorespiratory conditions (i.e. 18.4 kPa and 1.84 kPa atmospheric O-2 partial pressure, respectively), and in the dark. Under approximately current ambient O-2 partial pressure (18.4 kPa pO(2)), the gdch knockdown plants showed an expected photorespiratory-deficient phenotype, with lower leaf net CO2 assimilation rates (A) than the wild-type. Additionally, under these conditions, the gdch knockdown plants had greater leaf net discrimination against (CO2)-C-13 (Delta(o)) than the wild-type. This difference in Delta(o) was in part due to lower C-13 photorespiratory fractionation (f) ascribed to alternative decarboxylation of photorespiratory intermediates. Furthermore, the leaf dark respiration rate (R-d) was enhanced and the (CO2)-C-13 composition of respired CO2 (delta C-13(Rd)) showed a tendency to be more depleted in the gdch knockdown plants. These changes in R-d and delta C-13(Rd) were due to the amount and carbon isotopic composition of substrates available for dark respiration. These results demonstrate that impairment of the photorespiratory pathway affects leaf (CO2)-C-13 exchange, particularly the C-13 decarboxylation fractionation associated with photorespiration |
| format | Journal Article |
| id | CGSpace164716 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1647162025-05-14T10:23:58Z Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves Giuliani, Rita Karki, Shanta Covshoff, Sarah Lin, Hsiang-Chun Coe, Robert A. Koteyeva, Nuria K. Quick, W. Paul von Caemmerer, Susanne Furbank, Robert T. Hibberd, Julian M. Edwards, Gerald E. Cousins, Asaph B. The influence of reduced glycine decarboxylase complex (GDC) activity on leaf atmosphere CO2 and (CO2)-C-13 exchange was tested in transgenic Oryza sativa with the GDC H-subunit knocked down in leaf mesophyll cells. Leaf measurements on transgenic gdch knockdown and wild-type plants were carried out in the light under photorespiratory and low photorespiratory conditions (i.e. 18.4 kPa and 1.84 kPa atmospheric O-2 partial pressure, respectively), and in the dark. Under approximately current ambient O-2 partial pressure (18.4 kPa pO(2)), the gdch knockdown plants showed an expected photorespiratory-deficient phenotype, with lower leaf net CO2 assimilation rates (A) than the wild-type. Additionally, under these conditions, the gdch knockdown plants had greater leaf net discrimination against (CO2)-C-13 (Delta(o)) than the wild-type. This difference in Delta(o) was in part due to lower C-13 photorespiratory fractionation (f) ascribed to alternative decarboxylation of photorespiratory intermediates. Furthermore, the leaf dark respiration rate (R-d) was enhanced and the (CO2)-C-13 composition of respired CO2 (delta C-13(Rd)) showed a tendency to be more depleted in the gdch knockdown plants. These changes in R-d and delta C-13(Rd) were due to the amount and carbon isotopic composition of substrates available for dark respiration. These results demonstrate that impairment of the photorespiratory pathway affects leaf (CO2)-C-13 exchange, particularly the C-13 decarboxylation fractionation associated with photorespiration 2019-05-09 2024-12-19T12:54:13Z 2024-12-19T12:54:13Z Journal Article https://hdl.handle.net/10568/164716 en Open Access Oxford University Press Giuliani, Rita; Karki, Shanta; Covshoff, Sarah; Lin, Hsiang-Chun; Coe, Robert A; Koteyeva, Nuria K; Quick, W Paul; Von Caemmerer, Susanne; Furbank, Robert T; Hibberd, Julian M; Edwards, Gerald E and Cousins, Asaph B. 2019. Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves. Journal Of Experimental Botany, Volume 70, no. 10; pages 2773-2786. |
| spellingShingle | Giuliani, Rita Karki, Shanta Covshoff, Sarah Lin, Hsiang-Chun Coe, Robert A. Koteyeva, Nuria K. Quick, W. Paul von Caemmerer, Susanne Furbank, Robert T. Hibberd, Julian M. Edwards, Gerald E. Cousins, Asaph B. Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title | Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title_full | Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title_fullStr | Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title_full_unstemmed | Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title_short | Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves |
| title_sort | knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in oryza sativa leaves |
| url | https://hdl.handle.net/10568/164716 |
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