Investigation of host range of and host defense against a mitochondrially replicating mitovirus
Capsidless mitoviruses, which are ubiquitously detected in filamentous fungi, have the simplest RNA genomes of 2.2 to 4.4 kb, encoding only RNA-dependent RNA polymerase. Despite their simple genomes, detailed biological characterization of mitoviruses has been hampered by their mitochondrial locatio...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Society for Microbiology
2019
|
| Acceso en línea: | https://hdl.handle.net/10568/164712 |
| _version_ | 1855514385280139264 |
|---|---|
| author | Shahi, Sabitree Eusebio-Cope, Ana Kondō, Hideki Hillman, Bradley I. Suzuki, Nobuhiro |
| author_browse | Eusebio-Cope, Ana Hillman, Bradley I. Kondō, Hideki Shahi, Sabitree Suzuki, Nobuhiro |
| author_facet | Shahi, Sabitree Eusebio-Cope, Ana Kondō, Hideki Hillman, Bradley I. Suzuki, Nobuhiro |
| author_sort | Shahi, Sabitree |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Capsidless mitoviruses, which are ubiquitously detected in filamentous fungi, have the simplest RNA genomes of 2.2 to 4.4 kb, encoding only RNA-dependent RNA polymerase. Despite their simple genomes, detailed biological characterization of mitoviruses has been hampered by their mitochondrial location within the cell, posing challenges to their experimental introduction and study. Here we developed a protoplast fusion-based protocol for horizontal transfer of the prototype mitovirus, Cryphonectria parasitica mitovirus 1 (CpMV1), which was isolated from strain NB631 of the chestnut blight fungus ( Cryphonectria parasitica ), a model filamentous fungus for studying virus-host interactions. The host range of CpMV1 has been expanded to many different strains of C. parasitica and different fungal species within and outside the Cryphonectriaceae. Comparison of CpMV1 accumulation among various RNA silencing-deficient and -competent strains showed clearly that the virus was unaffected by RNA silencing. This study provides a solid foundation for further exploration of mitovirus-host interactions. |
| format | Journal Article |
| id | CGSpace164712 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace1647122025-05-14T10:23:49Z Investigation of host range of and host defense against a mitochondrially replicating mitovirus Shahi, Sabitree Eusebio-Cope, Ana Kondō, Hideki Hillman, Bradley I. Suzuki, Nobuhiro Capsidless mitoviruses, which are ubiquitously detected in filamentous fungi, have the simplest RNA genomes of 2.2 to 4.4 kb, encoding only RNA-dependent RNA polymerase. Despite their simple genomes, detailed biological characterization of mitoviruses has been hampered by their mitochondrial location within the cell, posing challenges to their experimental introduction and study. Here we developed a protoplast fusion-based protocol for horizontal transfer of the prototype mitovirus, Cryphonectria parasitica mitovirus 1 (CpMV1), which was isolated from strain NB631 of the chestnut blight fungus ( Cryphonectria parasitica ), a model filamentous fungus for studying virus-host interactions. The host range of CpMV1 has been expanded to many different strains of C. parasitica and different fungal species within and outside the Cryphonectriaceae. Comparison of CpMV1 accumulation among various RNA silencing-deficient and -competent strains showed clearly that the virus was unaffected by RNA silencing. This study provides a solid foundation for further exploration of mitovirus-host interactions. 2019-03-15 2024-12-19T12:54:13Z 2024-12-19T12:54:13Z Journal Article https://hdl.handle.net/10568/164712 en American Society for Microbiology Shahi, Sabitree; Eusebio-Cope, Ana; Kondo, Hideki; Hillman, Bradley I. and Suzuki, Nobuhiro. 2019. Investigation of host range of and host defense against a mitochondrially replicating mitovirus. J Virol, Volume 93, no. 6 |
| spellingShingle | Shahi, Sabitree Eusebio-Cope, Ana Kondō, Hideki Hillman, Bradley I. Suzuki, Nobuhiro Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title | Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title_full | Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title_fullStr | Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title_full_unstemmed | Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title_short | Investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| title_sort | investigation of host range of and host defense against a mitochondrially replicating mitovirus |
| url | https://hdl.handle.net/10568/164712 |
| work_keys_str_mv | AT shahisabitree investigationofhostrangeofandhostdefenseagainstamitochondriallyreplicatingmitovirus AT eusebiocopeana investigationofhostrangeofandhostdefenseagainstamitochondriallyreplicatingmitovirus AT kondohideki investigationofhostrangeofandhostdefenseagainstamitochondriallyreplicatingmitovirus AT hillmanbradleyi investigationofhostrangeofandhostdefenseagainstamitochondriallyreplicatingmitovirus AT suzukinobuhiro investigationofhostrangeofandhostdefenseagainstamitochondriallyreplicatingmitovirus |