A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems
Xanthomonas oryzae (Xo) are critical rice pathogens. Virulent lineages from Africa and Asia and less virulent strains from the US have been well characterized. X. campestris pv. leersiae (Xcl), first described in 1957, causes bacterial streak on the perennial grass, Leersia hexandra, and is a close...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2019
|
| Online Access: | https://hdl.handle.net/10568/164694 |
| _version_ | 1855516664955666432 |
|---|---|
| author | Lang, Jillian M. Pérez-Quintero, Alvaro L. Koebnik, Ralf DuCharme, Elysa Sarra, Soungalo Doucoure, Hinda Keita, Ibrahim Ziegle, Janet Jacobs, Jonathan M. Oliva, Ricardo Koita, Ousmane Szurek, Boris Verdier, Valérie Leach, Jan E. |
| author_browse | Doucoure, Hinda DuCharme, Elysa Jacobs, Jonathan M. Keita, Ibrahim Koebnik, Ralf Koita, Ousmane Lang, Jillian M. Leach, Jan E. Oliva, Ricardo Pérez-Quintero, Alvaro L. Sarra, Soungalo Szurek, Boris Verdier, Valérie Ziegle, Janet |
| author_facet | Lang, Jillian M. Pérez-Quintero, Alvaro L. Koebnik, Ralf DuCharme, Elysa Sarra, Soungalo Doucoure, Hinda Keita, Ibrahim Ziegle, Janet Jacobs, Jonathan M. Oliva, Ricardo Koita, Ousmane Szurek, Boris Verdier, Valérie Leach, Jan E. |
| author_sort | Lang, Jillian M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Xanthomonas oryzae (Xo) are critical rice pathogens. Virulent lineages from Africa and Asia and less virulent strains from the US have been well characterized. X. campestris pv. leersiae (Xcl), first described in 1957, causes bacterial streak on the perennial grass, Leersia hexandra, and is a close relative of Xo. L. hexandra, a member of the Poaceae, is highly similar to rice phylogenetically, is globally ubiquitous around rice paddies, and is a reservoir of pathogenic Xo. We used long read, single molecule, real time (SMRT) genome sequences of five strains of Xcl from Burkina Faso, China, Mali and Uganda to determine the genetic relatedness of this organism with Xo. Novel Transcription Activator-Like Effectors (TALEs) were discovered in all five strains of Xcl. Predicted TALE target sequences were identified in the L. perrieri genome and compared to rice susceptibility gene homologs. Pathogenicity screening on L. hexandra and diverse rice cultivars confirmed that Xcl are able to colonize rice and produce weak but not progressive symptoms. Overall, based on average nucleotide identity, type III effector repertoires and disease phenotype, we propose to rename Xcl to X. oryzae pv. leersiae (Xol) and use this parallel system to improve understanding of the evolution of bacterial pathogenicity in rice agroecosystems |
| format | Journal Article |
| id | CGSpace164694 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1646942024-12-19T14:11:58Z A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems Lang, Jillian M. Pérez-Quintero, Alvaro L. Koebnik, Ralf DuCharme, Elysa Sarra, Soungalo Doucoure, Hinda Keita, Ibrahim Ziegle, Janet Jacobs, Jonathan M. Oliva, Ricardo Koita, Ousmane Szurek, Boris Verdier, Valérie Leach, Jan E. Xanthomonas oryzae (Xo) are critical rice pathogens. Virulent lineages from Africa and Asia and less virulent strains from the US have been well characterized. X. campestris pv. leersiae (Xcl), first described in 1957, causes bacterial streak on the perennial grass, Leersia hexandra, and is a close relative of Xo. L. hexandra, a member of the Poaceae, is highly similar to rice phylogenetically, is globally ubiquitous around rice paddies, and is a reservoir of pathogenic Xo. We used long read, single molecule, real time (SMRT) genome sequences of five strains of Xcl from Burkina Faso, China, Mali and Uganda to determine the genetic relatedness of this organism with Xo. Novel Transcription Activator-Like Effectors (TALEs) were discovered in all five strains of Xcl. Predicted TALE target sequences were identified in the L. perrieri genome and compared to rice susceptibility gene homologs. Pathogenicity screening on L. hexandra and diverse rice cultivars confirmed that Xcl are able to colonize rice and produce weak but not progressive symptoms. Overall, based on average nucleotide identity, type III effector repertoires and disease phenotype, we propose to rename Xcl to X. oryzae pv. leersiae (Xol) and use this parallel system to improve understanding of the evolution of bacterial pathogenicity in rice agroecosystems 2019-04-30 2024-12-19T12:54:12Z 2024-12-19T12:54:12Z Journal Article https://hdl.handle.net/10568/164694 en Open Access Frontiers Media Lang, Jillian M.; Pérez-Quintero, Alvaro L.; Koebnik, Ralf; DuCharme, Elysa; Sarra, Soungalo; Doucoure, Hinda; Keita, Ibrahim; Ziegle, Janet; Jacobs, Jonathan M.; Oliva, Ricardo; Koita, Ousmane; Szurek, Boris; Verdier, Valérie and Leach, Jan E. 2019. A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems. Front. Plant Sci., Volume 10 |
| spellingShingle | Lang, Jillian M. Pérez-Quintero, Alvaro L. Koebnik, Ralf DuCharme, Elysa Sarra, Soungalo Doucoure, Hinda Keita, Ibrahim Ziegle, Janet Jacobs, Jonathan M. Oliva, Ricardo Koita, Ousmane Szurek, Boris Verdier, Valérie Leach, Jan E. A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title | A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title_full | A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title_fullStr | A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title_full_unstemmed | A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title_short | A pathovar of Xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| title_sort | pathovar of xanthomonas oryzae infecting wild grasses provides insight into the evolution of pathogenicity in rice agroecosystems |
| url | https://hdl.handle.net/10568/164694 |
| work_keys_str_mv | AT langjillianm apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT perezquinteroalvarol apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT koebnikralf apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT ducharmeelysa apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT sarrasoungalo apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT doucourehinda apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT keitaibrahim apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT zieglejanet apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT jacobsjonathanm apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT olivaricardo apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT koitaousmane apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT szurekboris apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT verdiervalerie apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT leachjane apathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT langjillianm pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT perezquinteroalvarol pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT koebnikralf pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT ducharmeelysa pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT sarrasoungalo pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT doucourehinda pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT keitaibrahim pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT zieglejanet pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT jacobsjonathanm pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT olivaricardo pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT koitaousmane pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT szurekboris pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT verdiervalerie pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems AT leachjane pathovarofxanthomonasoryzaeinfectingwildgrassesprovidesinsightintotheevolutionofpathogenicityinriceagroecosystems |