Variation in seed longevity among diverse Indica rice varieties
Understanding variation in seed longevity, especially within closely related germplasm, will lead to better understanding of the molecular basis of this trait, which is particularly important for seed genebanks, but is also relevant to anyone handling seeds. We therefore set out to determine the rel...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164670 |
| _version_ | 1855540325659967488 |
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| author | Lee, Jae-Sung Velasco-Punzalan, Marlina Pacleb, Myrish Valdez, Rocel Kretzschmar, Tobias McNally, Kenneth L. Ismail, Abdel M. Sta. Cruz, Pompe C. Hamilton, Ruaraidh Sackville Hay, Fiona R. |
| author_browse | Hamilton, Ruaraidh Sackville Hay, Fiona R. Ismail, Abdel M. Kretzschmar, Tobias Lee, Jae-Sung McNally, Kenneth L. Pacleb, Myrish Sta. Cruz, Pompe C. Valdez, Rocel Velasco-Punzalan, Marlina |
| author_facet | Lee, Jae-Sung Velasco-Punzalan, Marlina Pacleb, Myrish Valdez, Rocel Kretzschmar, Tobias McNally, Kenneth L. Ismail, Abdel M. Sta. Cruz, Pompe C. Hamilton, Ruaraidh Sackville Hay, Fiona R. |
| author_sort | Lee, Jae-Sung |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Understanding variation in seed longevity, especially within closely related germplasm, will lead to better understanding of the molecular basis of this trait, which is particularly important for seed genebanks, but is also relevant to anyone handling seeds. We therefore set out to determine the relative seed longevity of diverse Indica rice accessions through storage experiments. Since antioxidants are purported to play a role in seed storability, the antioxidant activity and phenolic content of caryopses were determined. Methods Seeds of 299 Indica rice accessions harvested at 31, 38 and 45 d after heading (DAH) between March and May 2015 and differing in harvest moisture content (MC) were subsequently stored at 10.9 % MC and 45 °C. Samples were taken at regular intervals and sown for germination. Germination data were subjected to probit analysis and the resulting parameters that describe the loss of viability during storage were used for genome-wide association (GWA) analysis. Key Results The seed longevity parameters, Ki [initial viability in normal equivalent deviates (NED)], −σ−1 (σ is the time for viability to fall by 1 NED in experimental storage) and p50 [time for viability to fall to 50 % (0 NED)], varied considerably across the 299 Indica accessions. Seed longevity tended to increase as harvest MC decreased and to decrease as harvest MC increased. Eight major loci associated with seed longevity parameters were identified through GWA analysis. The favourable haplotypes on chromosomes 1, 3, 4, 9 and 11 enhanced p50 by ratios of 0.22–1.86. This is the first study to describe the extent of variation in σ within a species’ variety group. A priori candidate genes selected based on rice genome annotation and gene network ontology databases suggested that the mechanisms conferring high seed longevity might be related to DNA repair and transcription, sugar metabolism, reactive oxygen species scavenging and embryonic/root development. |
| format | Journal Article |
| id | CGSpace164670 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1646702025-05-14T10:24:29Z Variation in seed longevity among diverse Indica rice varieties Lee, Jae-Sung Velasco-Punzalan, Marlina Pacleb, Myrish Valdez, Rocel Kretzschmar, Tobias McNally, Kenneth L. Ismail, Abdel M. Sta. Cruz, Pompe C. Hamilton, Ruaraidh Sackville Hay, Fiona R. plant science Understanding variation in seed longevity, especially within closely related germplasm, will lead to better understanding of the molecular basis of this trait, which is particularly important for seed genebanks, but is also relevant to anyone handling seeds. We therefore set out to determine the relative seed longevity of diverse Indica rice accessions through storage experiments. Since antioxidants are purported to play a role in seed storability, the antioxidant activity and phenolic content of caryopses were determined. Methods Seeds of 299 Indica rice accessions harvested at 31, 38 and 45 d after heading (DAH) between March and May 2015 and differing in harvest moisture content (MC) were subsequently stored at 10.9 % MC and 45 °C. Samples were taken at regular intervals and sown for germination. Germination data were subjected to probit analysis and the resulting parameters that describe the loss of viability during storage were used for genome-wide association (GWA) analysis. Key Results The seed longevity parameters, Ki [initial viability in normal equivalent deviates (NED)], −σ−1 (σ is the time for viability to fall by 1 NED in experimental storage) and p50 [time for viability to fall to 50 % (0 NED)], varied considerably across the 299 Indica accessions. Seed longevity tended to increase as harvest MC decreased and to decrease as harvest MC increased. Eight major loci associated with seed longevity parameters were identified through GWA analysis. The favourable haplotypes on chromosomes 1, 3, 4, 9 and 11 enhanced p50 by ratios of 0.22–1.86. This is the first study to describe the extent of variation in σ within a species’ variety group. A priori candidate genes selected based on rice genome annotation and gene network ontology databases suggested that the mechanisms conferring high seed longevity might be related to DNA repair and transcription, sugar metabolism, reactive oxygen species scavenging and embryonic/root development. 2019-10-18 2024-12-19T12:54:09Z 2024-12-19T12:54:09Z Journal Article https://hdl.handle.net/10568/164670 en Open Access Oxford University Press Lee, Jae-Sung; Velasco-Punzalan, Marlina; Pacleb, Myrish; Valdez, Rocel; Kretzschmar, Tobias; McNally, Kenneth L; Ismail, Abdel M; Cruz, Pompe C Sta; Sackville Hamilton, N Ruaraidh and Hay, Fiona R. 2019. Variation in seed longevity among diverse Indica rice varieties. Annals of Botany, (e-first copy); 42 pages |
| spellingShingle | plant science Lee, Jae-Sung Velasco-Punzalan, Marlina Pacleb, Myrish Valdez, Rocel Kretzschmar, Tobias McNally, Kenneth L. Ismail, Abdel M. Sta. Cruz, Pompe C. Hamilton, Ruaraidh Sackville Hay, Fiona R. Variation in seed longevity among diverse Indica rice varieties |
| title | Variation in seed longevity among diverse Indica rice varieties |
| title_full | Variation in seed longevity among diverse Indica rice varieties |
| title_fullStr | Variation in seed longevity among diverse Indica rice varieties |
| title_full_unstemmed | Variation in seed longevity among diverse Indica rice varieties |
| title_short | Variation in seed longevity among diverse Indica rice varieties |
| title_sort | variation in seed longevity among diverse indica rice varieties |
| topic | plant science |
| url | https://hdl.handle.net/10568/164670 |
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