Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines
The microbiomes of phloem‐feeding insects include functional bacteria and yeasts essential for herbivore survival and development. Changes in microbiome composition are implicated in virulence adaptation by herbivores to host plant species or host populations (including crop varieties). We examined...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164614 |
| _version_ | 1855533655355555840 |
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| author | Horgan, Finbarr G. Srinivasan, Thanga Suja Crisol-Martínez, Eduardo Almazan, Maria Liberty P. Ramal, Angelee Fame Oliva, Ricardo Quibod, Ian L. Bernal, Carmencita C. |
| author_browse | Almazan, Maria Liberty P. Bernal, Carmencita C. Crisol-Martínez, Eduardo Horgan, Finbarr G. Oliva, Ricardo Quibod, Ian L. Ramal, Angelee Fame Srinivasan, Thanga Suja |
| author_facet | Horgan, Finbarr G. Srinivasan, Thanga Suja Crisol-Martínez, Eduardo Almazan, Maria Liberty P. Ramal, Angelee Fame Oliva, Ricardo Quibod, Ian L. Bernal, Carmencita C. |
| author_sort | Horgan, Finbarr G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The microbiomes of phloem‐feeding insects include functional bacteria and yeasts essential for herbivore survival and development. Changes in microbiome composition are implicated in virulence adaptation by herbivores to host plant species or host populations (including crop varieties). We examined patterns in adaptation by the green leafhopper, Nephotettix virescens, to near‐isogenic rice lines (NILs) with one or two resistance genes and the recurrent parent T65, without resistance genes. Only the line with two resistance genes was effective in reducing leafhopper fitness. After 20 generations on the resistant line, selected leafhoppers attained similar survival, weight gain, and egg laying to leafhoppers that were continually reared on the susceptible recurrent parent, indicating that they had adapted to the resistant host. By sequencing the 16s rRNA gene, we described the microbiome of leafhoppers from colonies associated with five collection sites, and continually reared or switched between NILs. The microbiomes included 69–119 OTUs of which 44 occurred in ≥90% of samples. Of these, 14 OTUs were assigned to the obligate symbiont Candidatus sulcia clade. After 20 generations of selection, collection site had a greater effect than host plant on microbiome composition. Six bacteria genera, including C. sulcia, were associated with leafhopper virulence. However, there was significant within‐treatment, site‐related variability in the prevalence of these taxa such that the mechanisms underlying their association with virulence remain to be determined. Our results imply that these taxa are associated with leafhopper nutrition. Ours is the first study to describe microbiome diversity and composition in rice leafhoppers. We discuss our results in light of the multiple functions of herbivore microbiomes during virulence adaptation in insect herbivores. |
| format | Journal Article |
| id | CGSpace164614 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1646142025-05-14T10:24:20Z Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines Horgan, Finbarr G. Srinivasan, Thanga Suja Crisol-Martínez, Eduardo Almazan, Maria Liberty P. Ramal, Angelee Fame Oliva, Ricardo Quibod, Ian L. Bernal, Carmencita C. ecology ecology evolution behavior and systematics nature and landscape conservation The microbiomes of phloem‐feeding insects include functional bacteria and yeasts essential for herbivore survival and development. Changes in microbiome composition are implicated in virulence adaptation by herbivores to host plant species or host populations (including crop varieties). We examined patterns in adaptation by the green leafhopper, Nephotettix virescens, to near‐isogenic rice lines (NILs) with one or two resistance genes and the recurrent parent T65, without resistance genes. Only the line with two resistance genes was effective in reducing leafhopper fitness. After 20 generations on the resistant line, selected leafhoppers attained similar survival, weight gain, and egg laying to leafhoppers that were continually reared on the susceptible recurrent parent, indicating that they had adapted to the resistant host. By sequencing the 16s rRNA gene, we described the microbiome of leafhoppers from colonies associated with five collection sites, and continually reared or switched between NILs. The microbiomes included 69–119 OTUs of which 44 occurred in ≥90% of samples. Of these, 14 OTUs were assigned to the obligate symbiont Candidatus sulcia clade. After 20 generations of selection, collection site had a greater effect than host plant on microbiome composition. Six bacteria genera, including C. sulcia, were associated with leafhopper virulence. However, there was significant within‐treatment, site‐related variability in the prevalence of these taxa such that the mechanisms underlying their association with virulence remain to be determined. Our results imply that these taxa are associated with leafhopper nutrition. Ours is the first study to describe microbiome diversity and composition in rice leafhoppers. We discuss our results in light of the multiple functions of herbivore microbiomes during virulence adaptation in insect herbivores. 2019-10 2024-12-19T12:54:07Z 2024-12-19T12:54:07Z Journal Article https://hdl.handle.net/10568/164614 en Open Access Wiley Horgan, Finbarr G.; Srinivasan, Thanga Suja; Crisol‐Martínez, Eduardo; Almazan, Maria Liberty P.; Ramal, Angelee Fame; Oliva, Ricardo; Quibod, Ian L. and Bernal, Carmencita C. 2019. Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines. Ecology and Evolution, Volume 9 no. 20 p. 11911-11929 |
| spellingShingle | ecology ecology evolution behavior and systematics nature and landscape conservation Horgan, Finbarr G. Srinivasan, Thanga Suja Crisol-Martínez, Eduardo Almazan, Maria Liberty P. Ramal, Angelee Fame Oliva, Ricardo Quibod, Ian L. Bernal, Carmencita C. Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title | Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title_full | Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title_fullStr | Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title_full_unstemmed | Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title_short | Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines |
| title_sort | microbiome responses during virulence adaptation by a phloem feeding insect to resistant near isogenic rice lines |
| topic | ecology ecology evolution behavior and systematics nature and landscape conservation |
| url | https://hdl.handle.net/10568/164614 |
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