The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell
C4photosynthetic plants have evolved from C3ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C4enzymes either to mesophyll (M) or bundle sheath cells is considered a crucial step towards the evolution of C4photosynthesis. In this...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2020
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| Acceso en línea: | https://hdl.handle.net/10568/164607 |
| _version_ | 1855525809770463232 |
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| author | Gupta, Shipan Das Levey, Myles Schulze, Stefanie Karki, Shanta Emmerling, Jan Streubel, Monika Gowik, Udo Quick, W. Paul Westhoff, Peter |
| author_browse | Emmerling, Jan Gowik, Udo Gupta, Shipan Das Karki, Shanta Levey, Myles Quick, W. Paul Schulze, Stefanie Streubel, Monika Westhoff, Peter |
| author_facet | Gupta, Shipan Das Levey, Myles Schulze, Stefanie Karki, Shanta Emmerling, Jan Streubel, Monika Gowik, Udo Quick, W. Paul Westhoff, Peter |
| author_sort | Gupta, Shipan Das |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | C4photosynthetic plants have evolved from C3ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C4enzymes either to mesophyll (M) or bundle sheath cells is considered a crucial step towards the evolution of C4photosynthesis. In this study, we demonstrate that the 5′‐flanking sequences of the C4type phosphoenolpyruvate carboxylase (Ppc) gene from three C4grass species could drive M‐cell‐specific expression of a reporter gene in rice. In addition to that, we identified about 450 bp (upstream of their transcription start site) of the analyzedC4Ppcpromoters contain all the essential regulatory elements for driving M‐cell‐specific expression in rice leaves. Importantly, four motifs of conserved nucleotide sequences (CNSs) were also determined, which are essential for the activity of the promoter. A putative interaction between theCNSs and an unknown upstream element(s) is required for driving M‐cell‐specific expression. This work identifies the evolutionary conservation ofC4Ppcregulatory mechanisms of multiple closely related C4grass species. |
| format | Journal Article |
| id | CGSpace164607 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1646072025-12-08T09:54:28Z The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell Gupta, Shipan Das Levey, Myles Schulze, Stefanie Karki, Shanta Emmerling, Jan Streubel, Monika Gowik, Udo Quick, W. Paul Westhoff, Peter C4photosynthetic plants have evolved from C3ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C4enzymes either to mesophyll (M) or bundle sheath cells is considered a crucial step towards the evolution of C4photosynthesis. In this study, we demonstrate that the 5′‐flanking sequences of the C4type phosphoenolpyruvate carboxylase (Ppc) gene from three C4grass species could drive M‐cell‐specific expression of a reporter gene in rice. In addition to that, we identified about 450 bp (upstream of their transcription start site) of the analyzedC4Ppcpromoters contain all the essential regulatory elements for driving M‐cell‐specific expression in rice leaves. Importantly, four motifs of conserved nucleotide sequences (CNSs) were also determined, which are essential for the activity of the promoter. A putative interaction between theCNSs and an unknown upstream element(s) is required for driving M‐cell‐specific expression. This work identifies the evolutionary conservation ofC4Ppcregulatory mechanisms of multiple closely related C4grass species. 2020-01 2024-12-19T12:54:06Z 2024-12-19T12:54:06Z Journal Article https://hdl.handle.net/10568/164607 en Open Access Wiley Gupta, Shipan Das; Levey, Myles; Schulze, Stefanie; Karki, Shanta; Emmerling, Jan; Streubel, Monika; Gowik, Udo; Paul Quick, W. and Westhoff, Peter. 2020. The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell. The Plant Journal, Volume 101 no. 1 p. 204-216 |
| spellingShingle | Gupta, Shipan Das Levey, Myles Schulze, Stefanie Karki, Shanta Emmerling, Jan Streubel, Monika Gowik, Udo Quick, W. Paul Westhoff, Peter The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title | The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title_full | The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title_fullStr | The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title_full_unstemmed | The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title_short | The C4Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| title_sort | c4ppc promoters of many c4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell |
| url | https://hdl.handle.net/10568/164607 |
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