Diagnostic kit for rice blight resistance
Blight-resistant rice lines are the most effective solution for bacterial blight, caused byXanthomonas oryzaepv.oryzae(Xoo). Key resistance mechanisms involveSWEETgenes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) inSWEETg...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164606 |
| _version_ | 1855529711421095936 |
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| author | Eom, Joon-Seob Luo, Dangping Atienza-Grande, Genelou Yang, Jungil Ji, Chonghui Thi Luu, Van Huguet-Tapia, José C. Char, Si Nian Liu, Bo Nguyen, Hanna Schmidt, Sarah Maria Szurek, Boris Vera Cruz, Casiana White, Frank F. Oliva, Ricardo Yang, Bing Frommer, Wolf B. |
| author_browse | Atienza-Grande, Genelou Char, Si Nian Eom, Joon-Seob Frommer, Wolf B. Huguet-Tapia, José C. Ji, Chonghui Liu, Bo Luo, Dangping Nguyen, Hanna Oliva, Ricardo Schmidt, Sarah Maria Szurek, Boris Thi Luu, Van Vera Cruz, Casiana White, Frank F. Yang, Bing Yang, Jungil |
| author_facet | Eom, Joon-Seob Luo, Dangping Atienza-Grande, Genelou Yang, Jungil Ji, Chonghui Thi Luu, Van Huguet-Tapia, José C. Char, Si Nian Liu, Bo Nguyen, Hanna Schmidt, Sarah Maria Szurek, Boris Vera Cruz, Casiana White, Frank F. Oliva, Ricardo Yang, Bing Frommer, Wolf B. |
| author_sort | Eom, Joon-Seob |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Blight-resistant rice lines are the most effective solution for bacterial blight, caused byXanthomonas oryzaepv.oryzae(Xoo). Key resistance mechanisms involveSWEETgenes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) inSWEETgene promoters and induceSWEETgenes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include aSWEETpromoter database, RT–PCR primers for detectingSWEETinduction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight. |
| format | Journal Article |
| id | CGSpace164606 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1646062024-12-19T14:12:55Z Diagnostic kit for rice blight resistance Eom, Joon-Seob Luo, Dangping Atienza-Grande, Genelou Yang, Jungil Ji, Chonghui Thi Luu, Van Huguet-Tapia, José C. Char, Si Nian Liu, Bo Nguyen, Hanna Schmidt, Sarah Maria Szurek, Boris Vera Cruz, Casiana White, Frank F. Oliva, Ricardo Yang, Bing Frommer, Wolf B. applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine Blight-resistant rice lines are the most effective solution for bacterial blight, caused byXanthomonas oryzaepv.oryzae(Xoo). Key resistance mechanisms involveSWEETgenes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) inSWEETgene promoters and induceSWEETgenes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include aSWEETpromoter database, RT–PCR primers for detectingSWEETinduction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight. 2019-11 2024-12-19T12:54:06Z 2024-12-19T12:54:06Z Journal Article https://hdl.handle.net/10568/164606 en Open Access Springer Eom, Joon-Seob; Luo, Dangping; Atienza-Grande, Genelou; Yang, Jungil; Ji, Chonghui; Thi Luu, Van; Huguet-Tapia, José C.; Char, Si Nian; Liu, Bo; Nguyen, Hanna; Schmidt, Sarah Maria; Szurek, Boris; Vera Cruz, Casiana; White, Frank F.; Oliva, Ricardo; Yang, Bing and Frommer, Wolf B. 2019. Diagnostic kit for rice blight resistance. Nat Biotechnol, Volume 37 no. 11 p. 1372-1379 |
| spellingShingle | applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine Eom, Joon-Seob Luo, Dangping Atienza-Grande, Genelou Yang, Jungil Ji, Chonghui Thi Luu, Van Huguet-Tapia, José C. Char, Si Nian Liu, Bo Nguyen, Hanna Schmidt, Sarah Maria Szurek, Boris Vera Cruz, Casiana White, Frank F. Oliva, Ricardo Yang, Bing Frommer, Wolf B. Diagnostic kit for rice blight resistance |
| title | Diagnostic kit for rice blight resistance |
| title_full | Diagnostic kit for rice blight resistance |
| title_fullStr | Diagnostic kit for rice blight resistance |
| title_full_unstemmed | Diagnostic kit for rice blight resistance |
| title_short | Diagnostic kit for rice blight resistance |
| title_sort | diagnostic kit for rice blight resistance |
| topic | applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine |
| url | https://hdl.handle.net/10568/164606 |
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