Broad-spectrum resistance to bacterial blight in rice using genome editing
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164605 |
| _version_ | 1855514561404207104 |
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| author | Oliva, Ricardo Ji, Chonghui Atienza-Grande, Genelou Huguet-Tapia, José C. Perez-Quintero, Alvaro Li, Ting Eom, Joon-Seob Li, Chenhao Nguyen, Hanna Liu, Bo Auguy, Florence Sciallano, Coline Luu, Van T. Dossa, Gerbert S. Cunnac, Sébastien Schmidt, Sarah M. Slamet-Loedin, Inez H. Vera Cruz, Casiana Szurek, Boris Frommer, Wolf B. White, Frank F. Yang, Bing |
| author_browse | Atienza-Grande, Genelou Auguy, Florence Cunnac, Sébastien Dossa, Gerbert S. Eom, Joon-Seob Frommer, Wolf B. Huguet-Tapia, José C. Ji, Chonghui Li, Chenhao Li, Ting Liu, Bo Luu, Van T. Nguyen, Hanna Oliva, Ricardo Perez-Quintero, Alvaro Schmidt, Sarah M. Sciallano, Coline Slamet-Loedin, Inez H. Szurek, Boris Vera Cruz, Casiana White, Frank F. Yang, Bing |
| author_facet | Oliva, Ricardo Ji, Chonghui Atienza-Grande, Genelou Huguet-Tapia, José C. Perez-Quintero, Alvaro Li, Ting Eom, Joon-Seob Li, Chenhao Nguyen, Hanna Liu, Bo Auguy, Florence Sciallano, Coline Luu, Van T. Dossa, Gerbert S. Cunnac, Sébastien Schmidt, Sarah M. Slamet-Loedin, Inez H. Vera Cruz, Casiana Szurek, Boris Frommer, Wolf B. White, Frank F. Yang, Bing |
| author_sort | Oliva, Ricardo |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genesSWEET11,SWEET13andSWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all threeSWEETgene promoters. Editing was further informed by sequence analyses of TALe genes in 63Xoostrains, which revealed multiple TALe variants forSWEET13alleles. Mutations were also created inSWEET14, which is also targeted by two TALes from an AfricanXoolineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-editedSWEETpromoters endow rice lines with robust, broad-spectrum resistance. |
| format | Journal Article |
| id | CGSpace164605 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1646052024-12-19T14:11:48Z Broad-spectrum resistance to bacterial blight in rice using genome editing Oliva, Ricardo Ji, Chonghui Atienza-Grande, Genelou Huguet-Tapia, José C. Perez-Quintero, Alvaro Li, Ting Eom, Joon-Seob Li, Chenhao Nguyen, Hanna Liu, Bo Auguy, Florence Sciallano, Coline Luu, Van T. Dossa, Gerbert S. Cunnac, Sébastien Schmidt, Sarah M. Slamet-Loedin, Inez H. Vera Cruz, Casiana Szurek, Boris Frommer, Wolf B. White, Frank F. Yang, Bing applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genesSWEET11,SWEET13andSWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all threeSWEETgene promoters. Editing was further informed by sequence analyses of TALe genes in 63Xoostrains, which revealed multiple TALe variants forSWEET13alleles. Mutations were also created inSWEET14, which is also targeted by two TALes from an AfricanXoolineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-editedSWEETpromoters endow rice lines with robust, broad-spectrum resistance. 2019-11 2024-12-19T12:54:06Z 2024-12-19T12:54:06Z Journal Article https://hdl.handle.net/10568/164605 en Open Access Springer Oliva, Ricardo; Ji, Chonghui; Atienza-Grande, Genelou; Huguet-Tapia, José C.; Perez-Quintero, Alvaro; Li, Ting; Eom, Joon-Seob; Li, Chenhao; Nguyen, Hanna; Liu, Bo; Auguy, Florence; Sciallano, Coline; Luu, Van T.; Dossa, Gerbert S.; Cunnac, Sébastien; Schmidt, Sarah M.; Slamet-Loedin, Inez H.; Vera Cruz, Casiana; Szurek, Boris; Frommer, Wolf B.; White, Frank F. and Yang, Bing. 2019. Broad-spectrum resistance to bacterial blight in rice using genome editing. Nat Biotechnol, Volume 37 no. 11 p. 1344-1350 |
| spellingShingle | applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine Oliva, Ricardo Ji, Chonghui Atienza-Grande, Genelou Huguet-Tapia, José C. Perez-Quintero, Alvaro Li, Ting Eom, Joon-Seob Li, Chenhao Nguyen, Hanna Liu, Bo Auguy, Florence Sciallano, Coline Luu, Van T. Dossa, Gerbert S. Cunnac, Sébastien Schmidt, Sarah M. Slamet-Loedin, Inez H. Vera Cruz, Casiana Szurek, Boris Frommer, Wolf B. White, Frank F. Yang, Bing Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title | Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title_full | Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title_fullStr | Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title_full_unstemmed | Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title_short | Broad-spectrum resistance to bacterial blight in rice using genome editing |
| title_sort | broad spectrum resistance to bacterial blight in rice using genome editing |
| topic | applied microbiology and biotechnology bioengineering biomedical engineering biotechnology molecular medicine |
| url | https://hdl.handle.net/10568/164605 |
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