Development of the automated primer design workflow uniqprimer and diagnostic primers for the broad-host-range plant pathogen Dickeya dianthicola

Uniqprimer, a software pipeline developed in Python, was deployed as a user-friendly internet tool in Rice Galaxy for comparative genome analyses to design primer sets for PCRassays capable of detecting target bacterial taxa. The pipeline was trialed with Dickeya dianthicola, a destructive broad-hos...

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Bibliographic Details
Main Authors: Karim, Shaista, McNally, R. Ryan, Nasaruddin, Afnan S., DeReeper, Alexis, Mauleon, Ramil P., Charkowski, Amy O., Leach, Jan E., Ben-Hur, Asa, Triplett, Lindsay R.
Format: Journal Article
Language:Inglés
Published: Scientific Societies 2019
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Online Access:https://hdl.handle.net/10568/164603

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