Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice
Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani Kühn, continues to be an important and challenging rice disease worldwide. Here, we used genome-wide association studies over a high-density rice array to facilitate the identification of potential novel genes and quantitative...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164552 |
| _version_ | 1855541975216816128 |
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| author | Oreiro, Eula Gems Grimares, Earlyn Kate Atienza-Grande, Genelou Quibod, Ian Lorenzo Roman-Reyna, Veronica Oliva, Ricardo |
| author_browse | Atienza-Grande, Genelou Grimares, Earlyn Kate Oliva, Ricardo Oreiro, Eula Gems Quibod, Ian Lorenzo Roman-Reyna, Veronica |
| author_facet | Oreiro, Eula Gems Grimares, Earlyn Kate Atienza-Grande, Genelou Quibod, Ian Lorenzo Roman-Reyna, Veronica Oliva, Ricardo |
| author_sort | Oreiro, Eula Gems |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani Kühn, continues to be an important and challenging rice disease worldwide. Here, we used genome-wide association studies over a high-density rice array to facilitate the identification of potential novel genes and quantitative trait loci related to sheath blight resistance. We identified multiple regions that significantly associated with independent disease components in chromosomes 1, 4, and 11 under controlled condition. In particular, we investigated qLN1128, a quantitative trait locus enriched with defense-related genes that reduce disease lesions in a near-isogenic line. RNA profiling of the line carrying qLN1128 showed a number of differentially expressed genes related to the reactive oxygen species (ROS)-redox pathway. Histochemical staining revealed less ROS accumulation on the resistant line, suggesting efficient ROS deregulation that delays pathogen colonization. The detection of genomic regions controlling multiple mechanisms of resistance to sheath blight will provide tools to design effective breeding interventions in rice. |
| format | Journal Article |
| id | CGSpace164552 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1645522024-12-22T05:45:03Z Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice Oreiro, Eula Gems Grimares, Earlyn Kate Atienza-Grande, Genelou Quibod, Ian Lorenzo Roman-Reyna, Veronica Oliva, Ricardo agronomy crop science general medicine physiology Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani Kühn, continues to be an important and challenging rice disease worldwide. Here, we used genome-wide association studies over a high-density rice array to facilitate the identification of potential novel genes and quantitative trait loci related to sheath blight resistance. We identified multiple regions that significantly associated with independent disease components in chromosomes 1, 4, and 11 under controlled condition. In particular, we investigated qLN1128, a quantitative trait locus enriched with defense-related genes that reduce disease lesions in a near-isogenic line. RNA profiling of the line carrying qLN1128 showed a number of differentially expressed genes related to the reactive oxygen species (ROS)-redox pathway. Histochemical staining revealed less ROS accumulation on the resistant line, suggesting efficient ROS deregulation that delays pathogen colonization. The detection of genomic regions controlling multiple mechanisms of resistance to sheath blight will provide tools to design effective breeding interventions in rice. 2020-02 2024-12-19T12:54:01Z 2024-12-19T12:54:01Z Journal Article https://hdl.handle.net/10568/164552 en Scientific Societies Oreiro, Eula Gems; Grimares, Earlyn Kate; Atienza-Grande, Genelou; Quibod, Ian Lorenzo; Roman-Reyna, Veronica and Oliva, Ricardo. 2020. Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice. MPMI, Volume 33 no. 2 p. 212-222 |
| spellingShingle | agronomy crop science general medicine physiology Oreiro, Eula Gems Grimares, Earlyn Kate Atienza-Grande, Genelou Quibod, Ian Lorenzo Roman-Reyna, Veronica Oliva, Ricardo Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title | Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title_full | Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title_fullStr | Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title_full_unstemmed | Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title_short | Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice |
| title_sort | genome wide associations and transcriptional profiling reveal ros regulation as one underlying mechanism of sheath blight resistance in rice |
| topic | agronomy crop science general medicine physiology |
| url | https://hdl.handle.net/10568/164552 |
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