Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice
Unfavorable climatic changes have led to an increased threat of several biotic and abiotic stresses over the past few years. Looking at the massive damage caused by these stresses, we undertook a study to develop high yielding climate-resilient rice, using genes conferring resistance against blast (...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164500 |
| _version_ | 1855532004182851584 |
|---|---|
| author | Dixit, Shilpi Singh, Uma Maheshwar Singh, Arun Kumar Alam, Shamshad Venkateshwarlu, Challa Nachimuthu, Vishnu Varthini Yadav, Shailesh Abbai, Ragavendran Selvaraj, Ramchander Devi, M. Nagamallika Ramayya, Perumalla Janaki Badri, Jyothi Ram, T. Lakshmi, Jhansi Lakshmidevi, G. LRK, Jai Vidhya Padmakumari, Ayyagari Phani Laha, G.S. Prasad, M.S. Seetalam, Malathi Singh, Vikas Kumar Kumar, Arvind |
| author_browse | Abbai, Ragavendran Alam, Shamshad Badri, Jyothi Devi, M. Nagamallika Dixit, Shilpi Kumar, Arvind LRK, Jai Vidhya Laha, G.S. Lakshmi, Jhansi Lakshmidevi, G. Nachimuthu, Vishnu Varthini Padmakumari, Ayyagari Phani Prasad, M.S. Ram, T. Ramayya, Perumalla Janaki Seetalam, Malathi Selvaraj, Ramchander Singh, Arun Kumar Singh, Uma Maheshwar Singh, Vikas Kumar Venkateshwarlu, Challa Yadav, Shailesh |
| author_facet | Dixit, Shilpi Singh, Uma Maheshwar Singh, Arun Kumar Alam, Shamshad Venkateshwarlu, Challa Nachimuthu, Vishnu Varthini Yadav, Shailesh Abbai, Ragavendran Selvaraj, Ramchander Devi, M. Nagamallika Ramayya, Perumalla Janaki Badri, Jyothi Ram, T. Lakshmi, Jhansi Lakshmidevi, G. LRK, Jai Vidhya Padmakumari, Ayyagari Phani Laha, G.S. Prasad, M.S. Seetalam, Malathi Singh, Vikas Kumar Kumar, Arvind |
| author_sort | Dixit, Shilpi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Unfavorable climatic changes have led to an increased threat of several biotic and abiotic stresses over the past few years. Looking at the massive damage caused by these stresses, we undertook a study to develop high yielding climate-resilient rice, using genes conferring resistance against blast (Pi9), bacterial leaf blight (BLB) (Xa4, xa5, xa13, Xa21), brown planthopper (BPH) (Bph3, Bph17), gall midge (GM) (Gm4, Gm8) and QTLs for drought tolerance (qDTY1.1 and qDTY3.1) through marker-assisted forward breeding (MAFB) approach. Result Seven introgression lines (ILs) possessing a combination of seven to ten genes/QTLs for different biotic and abiotic stresses have been developed using marker-assisted selection (MAS) breeding method in the background of Swarna with drought QTLs. These ILs were superior to the respective recurrent parent in agronomic performance and also possess preferred grain quality with intermediate to high amylose content (AC) (23–26%). Out of these, three ILs viz., IL1 (Pi9+ Xa4+ xa5+ Xa21+ Bph17+ Gm8+ qDTY1.1+ qDTY3.1), IL6 (Pi9+ Xa4+ xa5+ Xa21+ Bph3+ Bph17+ Gm4+ Gm8+ qDTY1.1+ qDTY3.1) and IL7 (Pi9+ Xa4+ xa5+ Bph3+ Gm4+ qDTY1.1+ qDTY3.1) had shown resistance\tolerance for multiple biotic and abiotic stresses both in the field and glasshouse conditions. Overall, the ILs were high yielding under various stresses and importantly they also performed well in non-stress conditions without any yield penalty. Conclusion The current study clearly illustrated the success of MAS in combining tolerance to multiple biotic and abiotic stresses while maintaining higher yield potential and preferred grain quality. Developed ILs with seven to ten genes in the current study showed superiority to recurrent parent Swarna+drought for multiple-biotic stresses (blast, BLB, BPH and GM) together with yield advantages of 1.0 t ha− 1 under drought condition, without adverse effect on grain quality traits under non-stress. |
| format | Journal Article |
| id | CGSpace164500 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1645002025-05-14T10:39:50Z Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice Dixit, Shilpi Singh, Uma Maheshwar Singh, Arun Kumar Alam, Shamshad Venkateshwarlu, Challa Nachimuthu, Vishnu Varthini Yadav, Shailesh Abbai, Ragavendran Selvaraj, Ramchander Devi, M. Nagamallika Ramayya, Perumalla Janaki Badri, Jyothi Ram, T. Lakshmi, Jhansi Lakshmidevi, G. LRK, Jai Vidhya Padmakumari, Ayyagari Phani Laha, G.S. Prasad, M.S. Seetalam, Malathi Singh, Vikas Kumar Kumar, Arvind agronomy crop science plant science soil science Unfavorable climatic changes have led to an increased threat of several biotic and abiotic stresses over the past few years. Looking at the massive damage caused by these stresses, we undertook a study to develop high yielding climate-resilient rice, using genes conferring resistance against blast (Pi9), bacterial leaf blight (BLB) (Xa4, xa5, xa13, Xa21), brown planthopper (BPH) (Bph3, Bph17), gall midge (GM) (Gm4, Gm8) and QTLs for drought tolerance (qDTY1.1 and qDTY3.1) through marker-assisted forward breeding (MAFB) approach. Result Seven introgression lines (ILs) possessing a combination of seven to ten genes/QTLs for different biotic and abiotic stresses have been developed using marker-assisted selection (MAS) breeding method in the background of Swarna with drought QTLs. These ILs were superior to the respective recurrent parent in agronomic performance and also possess preferred grain quality with intermediate to high amylose content (AC) (23–26%). Out of these, three ILs viz., IL1 (Pi9+ Xa4+ xa5+ Xa21+ Bph17+ Gm8+ qDTY1.1+ qDTY3.1), IL6 (Pi9+ Xa4+ xa5+ Xa21+ Bph3+ Bph17+ Gm4+ Gm8+ qDTY1.1+ qDTY3.1) and IL7 (Pi9+ Xa4+ xa5+ Bph3+ Gm4+ qDTY1.1+ qDTY3.1) had shown resistance\tolerance for multiple biotic and abiotic stresses both in the field and glasshouse conditions. Overall, the ILs were high yielding under various stresses and importantly they also performed well in non-stress conditions without any yield penalty. Conclusion The current study clearly illustrated the success of MAS in combining tolerance to multiple biotic and abiotic stresses while maintaining higher yield potential and preferred grain quality. Developed ILs with seven to ten genes in the current study showed superiority to recurrent parent Swarna+drought for multiple-biotic stresses (blast, BLB, BPH and GM) together with yield advantages of 1.0 t ha− 1 under drought condition, without adverse effect on grain quality traits under non-stress. 2020-12 2024-12-19T12:53:59Z 2024-12-19T12:53:59Z Journal Article https://hdl.handle.net/10568/164500 en Open Access Springer Dixit, Shilpi; Singh, Uma Maheshwar; Singh, Arun Kumar; Alam, Shamshad; Venkateshwarlu, Challa; Nachimuthu, Vishnu Varthini; Yadav, Shailesh; Abbai, Ragavendran; Selvaraj, Ramchander; Devi, M. Nagamallika; Ramayya, Perumalla Janaki; Badri, Jyothi; Ram, T.; Lakshmi, Jhansi; Lakshmidevi, G.; LRK, Jai Vidhya; Padmakumari, Ayyagari Phani; Laha, G. S.; Prasad, M. S.; Seetalam, Malathi; Singh, Vikas Kumar and Kumar, Arvind. 2020. Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice. Rice, Volume 13, no. 1 |
| spellingShingle | agronomy crop science plant science soil science Dixit, Shilpi Singh, Uma Maheshwar Singh, Arun Kumar Alam, Shamshad Venkateshwarlu, Challa Nachimuthu, Vishnu Varthini Yadav, Shailesh Abbai, Ragavendran Selvaraj, Ramchander Devi, M. Nagamallika Ramayya, Perumalla Janaki Badri, Jyothi Ram, T. Lakshmi, Jhansi Lakshmidevi, G. LRK, Jai Vidhya Padmakumari, Ayyagari Phani Laha, G.S. Prasad, M.S. Seetalam, Malathi Singh, Vikas Kumar Kumar, Arvind Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title | Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title_full | Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title_fullStr | Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title_full_unstemmed | Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title_short | Marker assisted forward breeding to combine multiple biotic-abiotic stress resistance/tolerance in rice |
| title_sort | marker assisted forward breeding to combine multiple biotic abiotic stress resistance tolerance in rice |
| topic | agronomy crop science plant science soil science |
| url | https://hdl.handle.net/10568/164500 |
| work_keys_str_mv | AT dixitshilpi markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT singhumamaheshwar markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT singharunkumar markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT alamshamshad markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT venkateshwarluchalla markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT nachimuthuvishnuvarthini markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT yadavshailesh markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT abbairagavendran markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT selvarajramchander markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT devimnagamallika markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT ramayyaperumallajanaki markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT badrijyothi markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT ramt markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT lakshmijhansi markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT lakshmidevig markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT lrkjaividhya markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT padmakumariayyagariphani markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT lahags markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT prasadms markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT seetalammalathi markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT singhvikaskumar markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice AT kumararvind markerassistedforwardbreedingtocombinemultiplebioticabioticstressresistancetoleranceinrice |