Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves

Human malnutrition due to micronutrient deficiencies, particularly with regards to Zinc (Zn) and Selenium (Se), affects millions of people around the world, and the enrichment of staple foods through biofortification has been successfully used to fight hidden hunger. Rice (Oryza sativa L.) is one of...

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Main Authors: Roda, Faustino Adriano, Marques, Isabel, Batista-Santos, Paula, Esquível, Maria Glória, Ndayiragije, Alexis, Lidon, Fernando Cebola, Swamy, B.P. Mallikarjuna, Ramalho, José Cochicho, Ribeiro-Barros, Ana I.
Format: Journal Article
Language:Inglés
Published: Frontiers Media 2020
Subjects:
Online Access:https://hdl.handle.net/10568/164479
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author Roda, Faustino Adriano
Marques, Isabel
Batista-Santos, Paula
Esquível, Maria Glória
Ndayiragije, Alexis
Lidon, Fernando Cebola
Swamy, B.P. Mallikarjuna
Ramalho, José Cochicho
Ribeiro-Barros, Ana I.
author_browse Batista-Santos, Paula
Esquível, Maria Glória
Lidon, Fernando Cebola
Marques, Isabel
Ndayiragije, Alexis
Ramalho, José Cochicho
Ribeiro-Barros, Ana I.
Roda, Faustino Adriano
Swamy, B.P. Mallikarjuna
author_facet Roda, Faustino Adriano
Marques, Isabel
Batista-Santos, Paula
Esquível, Maria Glória
Ndayiragije, Alexis
Lidon, Fernando Cebola
Swamy, B.P. Mallikarjuna
Ramalho, José Cochicho
Ribeiro-Barros, Ana I.
author_sort Roda, Faustino Adriano
collection Repository of Agricultural Research Outputs (CGSpace)
description Human malnutrition due to micronutrient deficiencies, particularly with regards to Zinc (Zn) and Selenium (Se), affects millions of people around the world, and the enrichment of staple foods through biofortification has been successfully used to fight hidden hunger. Rice (Oryza sativa L.) is one of the staple foods most consumed in countries with high levels of malnutrition. However, it is poor in micronutrients, which are often removed during grain processing. In this study, we have analyzed the transcriptome of rice flag leaves biofortified with Zn (900 g ha–1), Se (500 g ha–1), and Zn-Se. Flag leaves play an important role in plant photosynthesis and provide sources of metal remobilization for developing grains. A total of 3170 differentially expressed genes (DEGs) were identified. The expression patterns and gene ontology of DEGs varied among the three sets of biofortified plants and were limited to specific metabolic pathways related to micronutrient mobilization and to the specific functions of Zn (i.e., its enzymatic co-factor/coenzyme function in the biosynthesis of nitrogenous compounds, carboxylic acids, organic acids, and amino acids) and Se (vitamin biosynthesis and ion homeostasis). The success of this approach should be followed in future studies to understand how landraces and other cultivars respond to biofortification.
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spelling CGSpace1644792025-05-14T10:24:19Z Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves Roda, Faustino Adriano Marques, Isabel Batista-Santos, Paula Esquível, Maria Glória Ndayiragije, Alexis Lidon, Fernando Cebola Swamy, B.P. Mallikarjuna Ramalho, José Cochicho Ribeiro-Barros, Ana I. genetics medical sciences Human malnutrition due to micronutrient deficiencies, particularly with regards to Zinc (Zn) and Selenium (Se), affects millions of people around the world, and the enrichment of staple foods through biofortification has been successfully used to fight hidden hunger. Rice (Oryza sativa L.) is one of the staple foods most consumed in countries with high levels of malnutrition. However, it is poor in micronutrients, which are often removed during grain processing. In this study, we have analyzed the transcriptome of rice flag leaves biofortified with Zn (900 g ha–1), Se (500 g ha–1), and Zn-Se. Flag leaves play an important role in plant photosynthesis and provide sources of metal remobilization for developing grains. A total of 3170 differentially expressed genes (DEGs) were identified. The expression patterns and gene ontology of DEGs varied among the three sets of biofortified plants and were limited to specific metabolic pathways related to micronutrient mobilization and to the specific functions of Zn (i.e., its enzymatic co-factor/coenzyme function in the biosynthesis of nitrogenous compounds, carboxylic acids, organic acids, and amino acids) and Se (vitamin biosynthesis and ion homeostasis). The success of this approach should be followed in future studies to understand how landraces and other cultivars respond to biofortification. 2020-07-07 2024-12-19T12:53:58Z 2024-12-19T12:53:58Z Journal Article https://hdl.handle.net/10568/164479 en Open Access Frontiers Media Roda, Faustino Adriano; Marques, Isabel; Batista-Santos, Paula; Esquível, Maria Glória; Ndayiragije, Alexis; Lidon, Fernando Cebola; Swamy, B. P. Mallikarjuna; Ramalho, José Cochicho and Ribeiro-Barros, Ana I. 2020. Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves. Front. Genet., Volume 11
spellingShingle genetics
medical sciences
Roda, Faustino Adriano
Marques, Isabel
Batista-Santos, Paula
Esquível, Maria Glória
Ndayiragije, Alexis
Lidon, Fernando Cebola
Swamy, B.P. Mallikarjuna
Ramalho, José Cochicho
Ribeiro-Barros, Ana I.
Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title_full Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title_fullStr Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title_full_unstemmed Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title_short Rice biofortification with zinc and selenium: a transcriptomic approach to understand mineral accumulation in flag leaves
title_sort rice biofortification with zinc and selenium a transcriptomic approach to understand mineral accumulation in flag leaves
topic genetics
medical sciences
url https://hdl.handle.net/10568/164479
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