Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater
Crops with resilience to multiple climatic stresses are essential for increased yield stability. Here, we evaluate the interaction between two loci associated with flooding survival in rice (Oryza sativa L.). ANAEROBIC GERMINATION 1 (AG1), encoding TREHALOSE 6‐PHOSPHATE PHOSPHATASE 7 (TPP7), promote...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2020
|
| Acceso en línea: | https://hdl.handle.net/10568/164471 |
| _version_ | 1855518022008045568 |
|---|---|
| author | Alam, Rejbana Hummel, Maureen Yeung, Elaine Locke, Anna M. Ignacio, John Carlos I. Baltazar, Miriam D. Jia, Zhenyu Ismail, Abdelbagi M. Septiningsih, Endang M. Bailey-Serres, Julia |
| author_browse | Alam, Rejbana Bailey-Serres, Julia Baltazar, Miriam D. Hummel, Maureen Ignacio, John Carlos I. Ismail, Abdelbagi M. Jia, Zhenyu Locke, Anna M. Septiningsih, Endang M. Yeung, Elaine |
| author_facet | Alam, Rejbana Hummel, Maureen Yeung, Elaine Locke, Anna M. Ignacio, John Carlos I. Baltazar, Miriam D. Jia, Zhenyu Ismail, Abdelbagi M. Septiningsih, Endang M. Bailey-Serres, Julia |
| author_sort | Alam, Rejbana |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Crops with resilience to multiple climatic stresses are essential for increased yield stability. Here, we evaluate the interaction between two loci associated with flooding survival in rice (Oryza sativa L.). ANAEROBIC GERMINATION 1 (AG1), encoding TREHALOSE 6‐PHOSPHATE PHOSPHATASE 7 (TPP7), promotes mobilization of endosperm reserves to enhance the elongation of a hollow coleoptile in seeds that are seeded directly into shallow paddies. SUBMERGENCE 1 (SUB1), encoding the ethylene‐responsive transcription factor SUB1A‐1, confers tolerance to complete submergence by dampening carbohydrate catabolism, to enhance recovery upon desubmergence. Interactions between AG1/TPP7 and SUB1/SUB1A‐1 were investigated under three flooding scenarios using four near‐isogenic lines by surveying growth and survival. Pyramiding of the two loci does not negatively affect anaerobic germination or vegetative‐stage submergence tolerance. However, the pyramided AG1 SUB1 genotype displays reduced survival when seeds are planted underwater and maintained under submergence for 16 d. To better understand the roles of TPP7 and SUB1A‐1 and their interaction, temporal changes in carbohydrates and shoot transcriptomes were monitored in the four genotypes varying at the two loci at four developmental timeponts, from day 2 after seeding through day 14 of complete submergence. TPP7 enhances early coleoptile elongation, whereas SUB1A‐1 promotes precocious photoautotrophy and then restricts underwater elongation. By contrast, pyramiding of the AG1 and SUB1 slows elongation growth, the transition to photoautotrophy, and survival. mRNA‐sequencing highlights time‐dependent and genotype‐specific regulation of mRNAs associated with DNA repair, cell cycle, chromatin modification, plastid biogenesis, carbohydrate catabolism and transport, elongation growth, and other processes. These results suggest that interactions between AG1/TPP7 and SUB1/SUB1A‐1 could impact seedling establishment if paddy depth is not effectively managed after direct seeding. |
| format | Journal Article |
| id | CGSpace164471 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1644712025-05-14T10:23:55Z Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater Alam, Rejbana Hummel, Maureen Yeung, Elaine Locke, Anna M. Ignacio, John Carlos I. Baltazar, Miriam D. Jia, Zhenyu Ismail, Abdelbagi M. Septiningsih, Endang M. Bailey-Serres, Julia Crops with resilience to multiple climatic stresses are essential for increased yield stability. Here, we evaluate the interaction between two loci associated with flooding survival in rice (Oryza sativa L.). ANAEROBIC GERMINATION 1 (AG1), encoding TREHALOSE 6‐PHOSPHATE PHOSPHATASE 7 (TPP7), promotes mobilization of endosperm reserves to enhance the elongation of a hollow coleoptile in seeds that are seeded directly into shallow paddies. SUBMERGENCE 1 (SUB1), encoding the ethylene‐responsive transcription factor SUB1A‐1, confers tolerance to complete submergence by dampening carbohydrate catabolism, to enhance recovery upon desubmergence. Interactions between AG1/TPP7 and SUB1/SUB1A‐1 were investigated under three flooding scenarios using four near‐isogenic lines by surveying growth and survival. Pyramiding of the two loci does not negatively affect anaerobic germination or vegetative‐stage submergence tolerance. However, the pyramided AG1 SUB1 genotype displays reduced survival when seeds are planted underwater and maintained under submergence for 16 d. To better understand the roles of TPP7 and SUB1A‐1 and their interaction, temporal changes in carbohydrates and shoot transcriptomes were monitored in the four genotypes varying at the two loci at four developmental timeponts, from day 2 after seeding through day 14 of complete submergence. TPP7 enhances early coleoptile elongation, whereas SUB1A‐1 promotes precocious photoautotrophy and then restricts underwater elongation. By contrast, pyramiding of the AG1 and SUB1 slows elongation growth, the transition to photoautotrophy, and survival. mRNA‐sequencing highlights time‐dependent and genotype‐specific regulation of mRNAs associated with DNA repair, cell cycle, chromatin modification, plastid biogenesis, carbohydrate catabolism and transport, elongation growth, and other processes. These results suggest that interactions between AG1/TPP7 and SUB1/SUB1A‐1 could impact seedling establishment if paddy depth is not effectively managed after direct seeding. 2020-07 2024-12-19T12:53:55Z 2024-12-19T12:53:55Z Journal Article https://hdl.handle.net/10568/164471 en Open Access Wiley Alam, Rejbana; Hummel, Maureen; Yeung, Elaine; Locke, Anna M.; Ignacio, John Carlos I.; Baltazar, Miriam D.; Jia, Zhenyu; Ismail, Abdelbagi M.; Septiningsih, Endang M. and Bailey‐Serres, Julia. 2020. Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater. Plant Direct, Volume 4, no. 7 |
| spellingShingle | Alam, Rejbana Hummel, Maureen Yeung, Elaine Locke, Anna M. Ignacio, John Carlos I. Baltazar, Miriam D. Jia, Zhenyu Ismail, Abdelbagi M. Septiningsih, Endang M. Bailey-Serres, Julia Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title | Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title_full | Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title_fullStr | Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title_full_unstemmed | Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title_short | Flood resilience loci SUBMERGENCE 1 and ANAEROBIC GERMINATION 1 interact in seedlings established underwater |
| title_sort | flood resilience loci submergence 1 and anaerobic germination 1 interact in seedlings established underwater |
| url | https://hdl.handle.net/10568/164471 |
| work_keys_str_mv | AT alamrejbana floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT hummelmaureen floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT yeungelaine floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT lockeannam floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT ignaciojohncarlosi floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT baltazarmiriamd floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT jiazhenyu floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT ismailabdelbagim floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT septiningsihendangm floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater AT baileyserresjulia floodresiliencelocisubmergence1andanaerobicgermination1interactinseedlingsestablishedunderwater |