Variation in seed metabolites between two indica rice accessions differing in seed longevity
For a better understanding of germination after seed storage, metabolite profiling was conducted using hybrid triple quadrupole time-of-flight (QTOF) mass spectrometry. After moisture content (MC) equilibration, seeds of “WAS170” (short-lived) and “IR65483” (long-lived) were stored at 10.9% MC and 4...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Acceso en línea: | https://hdl.handle.net/10568/164435 |
| _version_ | 1855517090513944576 |
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| author | Lee, Jae-Sung Hay, Fiona R. |
| author_browse | Hay, Fiona R. Lee, Jae-Sung |
| author_facet | Lee, Jae-Sung Hay, Fiona R. |
| author_sort | Lee, Jae-Sung |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | For a better understanding of germination after seed storage, metabolite profiling was conducted using hybrid triple quadrupole time-of-flight (QTOF) mass spectrometry. After moisture content (MC) equilibration, seeds of “WAS170” (short-lived) and “IR65483” (long-lived) were stored at 10.9% MC and 45 °C. Samples for metabolite analysis were taken after 0 and 20 days of storage. Among 288 metabolites, two flavonoids (kaempferide and quercetin-3-arabinoside), one amino acid (S-sulfocysteine) and one sugar (D-glucose) increased in “IR65483” seeds after storage but were not detected in “WAS170” seeds. Based on the genome sequence database, we identified clear allelic differences with non-synonymous mutations on the six flavonol synthase genes regulating the accumulation of kaempferol- and quercetin-metabolites. On the other hand, two metabolites (thiamine monophosphate and harmaline) increased in short-lived seeds after storage; these metabolites could be potential biochemical indicators of seed deterioration. |
| format | Journal Article |
| id | CGSpace164435 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1644352024-12-22T05:44:47Z Variation in seed metabolites between two indica rice accessions differing in seed longevity Lee, Jae-Sung Hay, Fiona R. For a better understanding of germination after seed storage, metabolite profiling was conducted using hybrid triple quadrupole time-of-flight (QTOF) mass spectrometry. After moisture content (MC) equilibration, seeds of “WAS170” (short-lived) and “IR65483” (long-lived) were stored at 10.9% MC and 45 °C. Samples for metabolite analysis were taken after 0 and 20 days of storage. Among 288 metabolites, two flavonoids (kaempferide and quercetin-3-arabinoside), one amino acid (S-sulfocysteine) and one sugar (D-glucose) increased in “IR65483” seeds after storage but were not detected in “WAS170” seeds. Based on the genome sequence database, we identified clear allelic differences with non-synonymous mutations on the six flavonol synthase genes regulating the accumulation of kaempferol- and quercetin-metabolites. On the other hand, two metabolites (thiamine monophosphate and harmaline) increased in short-lived seeds after storage; these metabolites could be potential biochemical indicators of seed deterioration. 2020-09-19 2024-12-19T12:53:53Z 2024-12-19T12:53:53Z Journal Article https://hdl.handle.net/10568/164435 en Open Access MDPI Lee, Jae-Sung; Hay, Fiona R. 2020. Variation in seed metabolites between two indica rice accessions differing in seed longevity. Plants, Volume 9 no. 9 p. 1237 |
| spellingShingle | Lee, Jae-Sung Hay, Fiona R. Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title | Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title_full | Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title_fullStr | Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title_full_unstemmed | Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title_short | Variation in seed metabolites between two indica rice accessions differing in seed longevity |
| title_sort | variation in seed metabolites between two indica rice accessions differing in seed longevity |
| url | https://hdl.handle.net/10568/164435 |
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