Non-destructive phenotyping for early seedling vigor in direct-seeded rice
Early seedling vigor is an essential trait of direct-seeded rice. It helps the seedlings to compete with weeds for water and nutrient availability, and contributes to better seedling establishment during the initial phase of crop growth. Seedling vigor is a complex trait, and phenotyping by a destru...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164434 |
| _version_ | 1855522676823556096 |
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| author | Anandan, Annamalai Mahender, Anumalla Sah, Rameswar Prasad Bose, Lotan Kumar Subudhi, Hatanath Meher, Jitendra Reddy, Janga Nagi Ali, Jauhar |
| author_browse | Ali, Jauhar Anandan, Annamalai Bose, Lotan Kumar Mahender, Anumalla Meher, Jitendra Reddy, Janga Nagi Sah, Rameswar Prasad Subudhi, Hatanath |
| author_facet | Anandan, Annamalai Mahender, Anumalla Sah, Rameswar Prasad Bose, Lotan Kumar Subudhi, Hatanath Meher, Jitendra Reddy, Janga Nagi Ali, Jauhar |
| author_sort | Anandan, Annamalai |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Early seedling vigor is an essential trait of direct-seeded rice. It helps the seedlings to compete with weeds for water and nutrient availability, and contributes to better seedling establishment during the initial phase of crop growth. Seedling vigor is a complex trait, and phenotyping by a destructive method limits the improvement of this trait through traditional breeding. Hence, a non-invasive, rapid, and precise image-based phenotyping technique is developed to increase the possibility to improve early seedling vigor through breeding in rice and other field crops.ResultsTo establish and assess the methodology using free-source software, early seedling vigor was estimated from images captured with a digital SLR camera in a non-destructive way. Here, the legitimacy and strength of the method have been proved through screening seven diverse rice cultivars varying for early seedling vigor. In the regression analysis, whole-plant area (WPA) estimated by destructive-flatbed scanner (WPAs) and non-destructive imaging (WPAi) approaches was strongly related (R2 > 83%) and suggested that WPAi can be adapted in place of destructive methods to estimate seedling vigor. In addition, this study has identified a set of new geometric traits (convex hull and top view area) for screening breeding lines for early seedling vigor in rice, which decreased the time by 80% and halved the cost of labor in data observation.ConclusionsThe method demonstrated here is affordable and easy to establish as a phenotypic platform. It is suitable for most glasshouses/net houses for characterizing genotypes to understand the plasticity of shoots under a given environment at the seedling stage. The methodology explained in this experiment has been proven to be practical and suggested as a technique for researchers involved in direct-seeded rice. Consequently, it will help in the simultaneous screening of genotypes in large numbers, the identification of donors, and in gaining information on the genetic basis of the trait to design a breeding program for direct-seeded rice. |
| format | Journal Article |
| id | CGSpace164434 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1644342024-12-19T14:12:24Z Non-destructive phenotyping for early seedling vigor in direct-seeded rice Anandan, Annamalai Mahender, Anumalla Sah, Rameswar Prasad Bose, Lotan Kumar Subudhi, Hatanath Meher, Jitendra Reddy, Janga Nagi Ali, Jauhar biotechnology genetics plant science Early seedling vigor is an essential trait of direct-seeded rice. It helps the seedlings to compete with weeds for water and nutrient availability, and contributes to better seedling establishment during the initial phase of crop growth. Seedling vigor is a complex trait, and phenotyping by a destructive method limits the improvement of this trait through traditional breeding. Hence, a non-invasive, rapid, and precise image-based phenotyping technique is developed to increase the possibility to improve early seedling vigor through breeding in rice and other field crops.ResultsTo establish and assess the methodology using free-source software, early seedling vigor was estimated from images captured with a digital SLR camera in a non-destructive way. Here, the legitimacy and strength of the method have been proved through screening seven diverse rice cultivars varying for early seedling vigor. In the regression analysis, whole-plant area (WPA) estimated by destructive-flatbed scanner (WPAs) and non-destructive imaging (WPAi) approaches was strongly related (R2 > 83%) and suggested that WPAi can be adapted in place of destructive methods to estimate seedling vigor. In addition, this study has identified a set of new geometric traits (convex hull and top view area) for screening breeding lines for early seedling vigor in rice, which decreased the time by 80% and halved the cost of labor in data observation.ConclusionsThe method demonstrated here is affordable and easy to establish as a phenotypic platform. It is suitable for most glasshouses/net houses for characterizing genotypes to understand the plasticity of shoots under a given environment at the seedling stage. The methodology explained in this experiment has been proven to be practical and suggested as a technique for researchers involved in direct-seeded rice. Consequently, it will help in the simultaneous screening of genotypes in large numbers, the identification of donors, and in gaining information on the genetic basis of the trait to design a breeding program for direct-seeded rice. 2020-12 2024-12-19T12:53:52Z 2024-12-19T12:53:52Z Journal Article https://hdl.handle.net/10568/164434 en Open Access Springer Anandan, Annamalai; Mahender, Anumalla; Sah, Rameswar Prasad; Bose, Lotan Kumar; Subudhi, Hatanath; Meher, Jitendra; Reddy, Janga Nagi and Ali, Jauhar. 2020. Non-destructive phenotyping for early seedling vigor in direct-seeded rice. Plant Methods, Volume 16, no. 1 |
| spellingShingle | biotechnology genetics plant science Anandan, Annamalai Mahender, Anumalla Sah, Rameswar Prasad Bose, Lotan Kumar Subudhi, Hatanath Meher, Jitendra Reddy, Janga Nagi Ali, Jauhar Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title | Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title_full | Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title_fullStr | Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title_full_unstemmed | Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title_short | Non-destructive phenotyping for early seedling vigor in direct-seeded rice |
| title_sort | non destructive phenotyping for early seedling vigor in direct seeded rice |
| topic | biotechnology genetics plant science |
| url | https://hdl.handle.net/10568/164434 |
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