Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.)
Rice (Oryza sativa L.) is more sensitive to drought stress than other cereals. To dissect molecular mechanisms underlying drought-tolerant yield in rice, we applied differential expression and co-expression network approaches to transcriptomes from flag-leaf and emerging panicle tissues of a drought...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164432 |
| _version_ | 1855541164128600064 |
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| author | Tarun, Jeshurun Asher Mauleon, Ramil Arbelaez, Juan David Catausan, Sheryl Dixit, Shalabh Kumar, Arvind Brown, Patrick Kohli, Ajay Kretzschmar, Tobias |
| author_browse | Arbelaez, Juan David Brown, Patrick Catausan, Sheryl Dixit, Shalabh Kohli, Ajay Kretzschmar, Tobias Kumar, Arvind Mauleon, Ramil Tarun, Jeshurun Asher |
| author_facet | Tarun, Jeshurun Asher Mauleon, Ramil Arbelaez, Juan David Catausan, Sheryl Dixit, Shalabh Kumar, Arvind Brown, Patrick Kohli, Ajay Kretzschmar, Tobias |
| author_sort | Tarun, Jeshurun Asher |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice (Oryza sativa L.) is more sensitive to drought stress than other cereals. To dissect molecular mechanisms underlying drought-tolerant yield in rice, we applied differential expression and co-expression network approaches to transcriptomes from flag-leaf and emerging panicle tissues of a drought-tolerant yield introgression line, DTY-IL, and the recurrent parent Swarna, under moderate reproductive-stage drought stress. Protein turnover and efficient reactive oxygen species scavenging were found to be the driving factors in both tissues. In the flag-leaf, the responses further included maintenance of photosynthesis and cell wall reorganization, while in the panicle biosynthesis of secondary metabolites was found to play additional roles. Hub genes of importance in differential drought responses included an expansin in the flag-leaf and two peroxidases in the panicle. Overlaying differential expression data with allelic variation in DTY-IL quantitative trait loci allowed for the prioritization of candidate genes. They included a differentially regulated auxin-responsive protein, with DTY-IL-specific amino acid changes in conserved domains, as well as a protein kinase with a DTY-IL-specific frameshift in the C-terminal region. The approach highlights how the integration of differential expression and allelic variation can aid in the discovery of mechanism and putative causal contribution underlying quantitative trait loci for drought-tolerant yield. |
| format | Journal Article |
| id | CGSpace164432 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1644322024-12-22T05:45:03Z Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) Tarun, Jeshurun Asher Mauleon, Ramil Arbelaez, Juan David Catausan, Sheryl Dixit, Shalabh Kumar, Arvind Brown, Patrick Kohli, Ajay Kretzschmar, Tobias genetics genetics(clinical) Rice (Oryza sativa L.) is more sensitive to drought stress than other cereals. To dissect molecular mechanisms underlying drought-tolerant yield in rice, we applied differential expression and co-expression network approaches to transcriptomes from flag-leaf and emerging panicle tissues of a drought-tolerant yield introgression line, DTY-IL, and the recurrent parent Swarna, under moderate reproductive-stage drought stress. Protein turnover and efficient reactive oxygen species scavenging were found to be the driving factors in both tissues. In the flag-leaf, the responses further included maintenance of photosynthesis and cell wall reorganization, while in the panicle biosynthesis of secondary metabolites was found to play additional roles. Hub genes of importance in differential drought responses included an expansin in the flag-leaf and two peroxidases in the panicle. Overlaying differential expression data with allelic variation in DTY-IL quantitative trait loci allowed for the prioritization of candidate genes. They included a differentially regulated auxin-responsive protein, with DTY-IL-specific amino acid changes in conserved domains, as well as a protein kinase with a DTY-IL-specific frameshift in the C-terminal region. The approach highlights how the integration of differential expression and allelic variation can aid in the discovery of mechanism and putative causal contribution underlying quantitative trait loci for drought-tolerant yield. 2020-09-24 2024-12-19T12:53:52Z 2024-12-19T12:53:52Z Journal Article https://hdl.handle.net/10568/164432 en Open Access MDPI Tarun, Jeshurun Asher; Mauleon, Ramil; Arbelaez, Juan David; Catausan, Sheryl; Dixit, Shalabh; Kumar, Arvind; Brown, Patrick; Kohli, Ajay and Kretzschmar, Tobias. 2020. Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.). Genes, Volume 11 no. 10 p. 1124 |
| spellingShingle | genetics genetics(clinical) Tarun, Jeshurun Asher Mauleon, Ramil Arbelaez, Juan David Catausan, Sheryl Dixit, Shalabh Kumar, Arvind Brown, Patrick Kohli, Ajay Kretzschmar, Tobias Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title | Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title_full | Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title_fullStr | Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title_full_unstemmed | Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title_short | Comparative transcriptomics and co-expression networks reveal tissue- and genotype-specific responses of qDTYs to reproductive-stage drought stress in rice (Oryza sativa L.) |
| title_sort | comparative transcriptomics and co expression networks reveal tissue and genotype specific responses of qdtys to reproductive stage drought stress in rice oryza sativa l |
| topic | genetics genetics(clinical) |
| url | https://hdl.handle.net/10568/164432 |
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