Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance

Iron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is indigenous to northern Australia and grows in regions with Fe-rich soils, making it a candidate for use in adaptive breeding. With the aim of under...

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Autores principales: Wairich, Andriele, de Oliveira, Ben Hur Neves, Wu, Lin-Bo, Murugaiyan, Varunseelan, Margis-Pinheiro, Marcia, Fett, Janette Palma, Ricachenevsky, Felipe Klein, Frei, Michael
Formato: Journal Article
Lenguaje:Inglés
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://hdl.handle.net/10568/164418
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author Wairich, Andriele
de Oliveira, Ben Hur Neves
Wu, Lin-Bo
Murugaiyan, Varunseelan
Margis-Pinheiro, Marcia
Fett, Janette Palma
Ricachenevsky, Felipe Klein
Frei, Michael
author_browse Fett, Janette Palma
Frei, Michael
Margis-Pinheiro, Marcia
Murugaiyan, Varunseelan
Ricachenevsky, Felipe Klein
Wairich, Andriele
Wu, Lin-Bo
de Oliveira, Ben Hur Neves
author_facet Wairich, Andriele
de Oliveira, Ben Hur Neves
Wu, Lin-Bo
Murugaiyan, Varunseelan
Margis-Pinheiro, Marcia
Fett, Janette Palma
Ricachenevsky, Felipe Klein
Frei, Michael
author_sort Wairich, Andriele
collection Repository of Agricultural Research Outputs (CGSpace)
description Iron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is indigenous to northern Australia and grows in regions with Fe-rich soils, making it a candidate for use in adaptive breeding. With the aim of understanding tolerance mechanisms in rice, we screened a population of interspecific introgression lines from a cross between O. sativa and O. meridionalis for the identification of quantitative trait loci (QTLs) contributing to Fe-toxicity tolerance. Six putative QTLs were identified. A line carrying one introgression from O. meridionalis on chromosome 9 associated with one QTL was highly tolerant despite very high shoot Fe concentrations. Physiological, biochemical, ionomic, and transcriptomic analyses showed that the tolerance of the introgression lines could partly be explained by higher relative Fe retention in the leaf sheath and culm. We constructed the interspecific hybrid genome in silico for transcriptomic analysis and identified differentially regulated introgressed genes from O. meridionalis that could be involved in shoot-based Fe tolerance, such as metallothioneins, glutathione S-transferases, and transporters from the ABC and MFS families. This work demonstrates that introgressions of O. meridionalis into the O. sativa genome can confer increased tolerance to excess Fe.
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spelling CGSpace1644182025-12-08T09:54:28Z Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance Wairich, Andriele de Oliveira, Ben Hur Neves Wu, Lin-Bo Murugaiyan, Varunseelan Margis-Pinheiro, Marcia Fett, Janette Palma Ricachenevsky, Felipe Klein Frei, Michael physiology plant science Iron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is indigenous to northern Australia and grows in regions with Fe-rich soils, making it a candidate for use in adaptive breeding. With the aim of understanding tolerance mechanisms in rice, we screened a population of interspecific introgression lines from a cross between O. sativa and O. meridionalis for the identification of quantitative trait loci (QTLs) contributing to Fe-toxicity tolerance. Six putative QTLs were identified. A line carrying one introgression from O. meridionalis on chromosome 9 associated with one QTL was highly tolerant despite very high shoot Fe concentrations. Physiological, biochemical, ionomic, and transcriptomic analyses showed that the tolerance of the introgression lines could partly be explained by higher relative Fe retention in the leaf sheath and culm. We constructed the interspecific hybrid genome in silico for transcriptomic analysis and identified differentially regulated introgressed genes from O. meridionalis that could be involved in shoot-based Fe tolerance, such as metallothioneins, glutathione S-transferases, and transporters from the ABC and MFS families. This work demonstrates that introgressions of O. meridionalis into the O. sativa genome can confer increased tolerance to excess Fe. 2021-03-17 2024-12-19T12:53:52Z 2024-12-19T12:53:52Z Journal Article https://hdl.handle.net/10568/164418 en Oxford University Press Wairich, Andriele; de Oliveira, Ben Hur Neves; Wu, Lin-Bo; Murugaiyan, Varunseelan; Margis-Pinheiro, Marcia; Fett, Janette Palma; Ricachenevsky, Felipe Klein and Frei, Michael. 2021. Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance. Journal of Experimental Botany, eraa461 [manuscript copy]
spellingShingle physiology
plant science
Wairich, Andriele
de Oliveira, Ben Hur Neves
Wu, Lin-Bo
Murugaiyan, Varunseelan
Margis-Pinheiro, Marcia
Fett, Janette Palma
Ricachenevsky, Felipe Klein
Frei, Michael
Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title_full Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title_fullStr Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title_full_unstemmed Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title_short Chromosomal introgressions from Oryza meridionalis into domesticated rice Oryza sativa result in iron tolerance
title_sort chromosomal introgressions from oryza meridionalis into domesticated rice oryza sativa result in iron tolerance
topic physiology
plant science
url https://hdl.handle.net/10568/164418
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