Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia
Rice orange leaf phytoplasma (ROLP) causes clear orange to yellowish leaf discoloration and severe stunting in rice seedlings. The ecological and biological characteristics of ROLP are largely unknown because the disease has not widely caused serious problems in rice cultivated areas, thereby leadin...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2021
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| Online Access: | https://hdl.handle.net/10568/164348 |
| _version_ | 1855518084880662528 |
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| author | Ong, Socheath Jonson, Gilda B. Calassanzio, Matteo Rin, Soriya Chou, Cheythyrith Oi, Takao Sato, Ikuo Takemoto, Daigo Tanaka, Toshiharu Choi, Il-Ryong Nign, Chhay Chiba, Sotaro |
| author_browse | Calassanzio, Matteo Chiba, Sotaro Choi, Il-Ryong Chou, Cheythyrith Jonson, Gilda B. Nign, Chhay Oi, Takao Ong, Socheath Rin, Soriya Sato, Ikuo Takemoto, Daigo Tanaka, Toshiharu |
| author_facet | Ong, Socheath Jonson, Gilda B. Calassanzio, Matteo Rin, Soriya Chou, Cheythyrith Oi, Takao Sato, Ikuo Takemoto, Daigo Tanaka, Toshiharu Choi, Il-Ryong Nign, Chhay Chiba, Sotaro |
| author_sort | Ong, Socheath |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice orange leaf phytoplasma (ROLP) causes clear orange to yellowish leaf discoloration and severe stunting in rice seedlings. The ecological and biological characteristics of ROLP are largely unknown because the disease has not widely caused serious problems in rice cultivated areas, thereby leading to the low accumulation of research data. However, in the past decade, the disease became a threat to rice production, particularly in South China and India; it has also been recognised in other Asian countries, such as Vietnam, Thailand and the Philippines. Here, we observed the occurrence of ROLP in paddies of the Southeast Asian counties (Cambodia, Vietnam and the Philippines) and found that the isolates in the Philippines and Vietnam were monophyletic, while those in India, Thailand and Cambodia were more diverse, suggesting their potential origins. In Cambodia, it was revealed that following polymerase chain reaction (PCR) detection, the known ROLP-insect vectors, N. virescens Distant and Recilia dorsalis Motchulsky, were ROLP-positive, indicating their roles in pathogen dispersal. Moreover, fluorescent and scanning electron microscopy revealed the intensive accumulation of the phytoplasma in phloem tissues and massive accumulation of storage starch in vascular bundle sheath and parenchyma. Altogether, this study illustrated the genetic variability of global ROLP isolates and the pathogen’s biological impact on rice tissue. |
| format | Journal Article |
| id | CGSpace164348 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1643482024-12-22T05:44:47Z Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia Ong, Socheath Jonson, Gilda B. Calassanzio, Matteo Rin, Soriya Chou, Cheythyrith Oi, Takao Sato, Ikuo Takemoto, Daigo Tanaka, Toshiharu Choi, Il-Ryong Nign, Chhay Chiba, Sotaro Rice orange leaf phytoplasma (ROLP) causes clear orange to yellowish leaf discoloration and severe stunting in rice seedlings. The ecological and biological characteristics of ROLP are largely unknown because the disease has not widely caused serious problems in rice cultivated areas, thereby leading to the low accumulation of research data. However, in the past decade, the disease became a threat to rice production, particularly in South China and India; it has also been recognised in other Asian countries, such as Vietnam, Thailand and the Philippines. Here, we observed the occurrence of ROLP in paddies of the Southeast Asian counties (Cambodia, Vietnam and the Philippines) and found that the isolates in the Philippines and Vietnam were monophyletic, while those in India, Thailand and Cambodia were more diverse, suggesting their potential origins. In Cambodia, it was revealed that following polymerase chain reaction (PCR) detection, the known ROLP-insect vectors, N. virescens Distant and Recilia dorsalis Motchulsky, were ROLP-positive, indicating their roles in pathogen dispersal. Moreover, fluorescent and scanning electron microscopy revealed the intensive accumulation of the phytoplasma in phloem tissues and massive accumulation of storage starch in vascular bundle sheath and parenchyma. Altogether, this study illustrated the genetic variability of global ROLP isolates and the pathogen’s biological impact on rice tissue. 2021-02-04 2024-12-19T12:53:45Z 2024-12-19T12:53:45Z Journal Article https://hdl.handle.net/10568/164348 en Open Access MDPI Ong, Socheath; Jonson, Gilda B.; Calassanzio, Matteo; Rin, Soriya; Chou, Cheythyrith; Oi, Takao; Sato, Ikuo; Takemoto, Daigo; Tanaka, Toshiharu; Choi, Il-Ryong; Nign, Chhay and Chiba, Sotaro. 2021. Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia. Pathogens, Volume 10 no. 2 p. 169 |
| spellingShingle | Ong, Socheath Jonson, Gilda B. Calassanzio, Matteo Rin, Soriya Chou, Cheythyrith Oi, Takao Sato, Ikuo Takemoto, Daigo Tanaka, Toshiharu Choi, Il-Ryong Nign, Chhay Chiba, Sotaro Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title | Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title_full | Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title_fullStr | Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title_full_unstemmed | Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title_short | Geographic distribution, genetic variability and biological properties of rice orange leaf phytoplasma in Southeast Asia |
| title_sort | geographic distribution genetic variability and biological properties of rice orange leaf phytoplasma in southeast asia |
| url | https://hdl.handle.net/10568/164348 |
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