A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome
Drought stress in plants causes differential expression of numerous genes. One of these differentially expressed genes in rice is a specific amidohydrolase. We characterized this amidohydrolase gene on the rice chromosome 12 as the first plantguanine deaminase(OsGDA1). The biochemical activity of GD...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164306 |
| _version_ | 1855542445245202432 |
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| author | Gotarkar, Dhananjay Longkumer, Toshisangba Yamamoto, Naoki Nanda, Amrit Kaur Iglesias, Tamara Li, Lin-Feng Miro, Berta Blanco Gonzalez, Elisa Montes Bayon, Maria Olsen, Kenneth M. Hsing, Yue-Ie Caroline Kohli, Ajay |
| author_browse | Blanco Gonzalez, Elisa Gotarkar, Dhananjay Hsing, Yue-Ie Caroline Iglesias, Tamara Kohli, Ajay Li, Lin-Feng Longkumer, Toshisangba Miro, Berta Montes Bayon, Maria Nanda, Amrit Kaur Olsen, Kenneth M. Yamamoto, Naoki |
| author_facet | Gotarkar, Dhananjay Longkumer, Toshisangba Yamamoto, Naoki Nanda, Amrit Kaur Iglesias, Tamara Li, Lin-Feng Miro, Berta Blanco Gonzalez, Elisa Montes Bayon, Maria Olsen, Kenneth M. Hsing, Yue-Ie Caroline Kohli, Ajay |
| author_sort | Gotarkar, Dhananjay |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought stress in plants causes differential expression of numerous genes. One of these differentially expressed genes in rice is a specific amidohydrolase. We characterized this amidohydrolase gene on the rice chromosome 12 as the first plantguanine deaminase(OsGDA1). The biochemical activity of GDA is known from tea and coffee plants where its catalytic product, xanthine, is the precursor for theine and caffeine. However, no plant gene that is coding for GDA is known so far. RecombinantOsGDA1converted guanine to xanthine in vitro. Measurement of guanine and xanthine contents in theOsGDA1knockout (KO) line and in the wild type Tainung 67 rice plants also suggested GDA activity in vivo. The content of cellular xanthine is important because of its catabolic products allantoin, ureides, and urea which play roles in water and nitrogen stress tolerance among others. The identification ofOsGDA1fills a critical gap in the S‐adenosyl‐methionine (SAM) to xanthine pathway. SAM is converted to S‐adenosyl‐homocysteine (SAH) and finally to xanthine. SAH is a potent inhibitor of DNA methyltransferases, the reduction of which leads to increased DNA methylation and gene silencing in Arabidopsis. We report that theOsGDA1KO line exhibited a decrease in SAM, SAH and adenosine and an increase in rice genome methylation. The OsGDA1 protein phylogeny combined with mutational protein destabilization analysis suggested artificial selection for null mutants, which could affect genome methylation as in the KO line. Limited information on genes that may affect epigenetics indirectly requires deeper insights into such a role and effect of purine catabolism and related genetic networks. |
| format | Journal Article |
| id | CGSpace164306 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1643062025-05-14T10:24:32Z A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome Gotarkar, Dhananjay Longkumer, Toshisangba Yamamoto, Naoki Nanda, Amrit Kaur Iglesias, Tamara Li, Lin-Feng Miro, Berta Blanco Gonzalez, Elisa Montes Bayon, Maria Olsen, Kenneth M. Hsing, Yue-Ie Caroline Kohli, Ajay cell biology general medicine genetics physiology plant science Drought stress in plants causes differential expression of numerous genes. One of these differentially expressed genes in rice is a specific amidohydrolase. We characterized this amidohydrolase gene on the rice chromosome 12 as the first plantguanine deaminase(OsGDA1). The biochemical activity of GDA is known from tea and coffee plants where its catalytic product, xanthine, is the precursor for theine and caffeine. However, no plant gene that is coding for GDA is known so far. RecombinantOsGDA1converted guanine to xanthine in vitro. Measurement of guanine and xanthine contents in theOsGDA1knockout (KO) line and in the wild type Tainung 67 rice plants also suggested GDA activity in vivo. The content of cellular xanthine is important because of its catabolic products allantoin, ureides, and urea which play roles in water and nitrogen stress tolerance among others. The identification ofOsGDA1fills a critical gap in the S‐adenosyl‐methionine (SAM) to xanthine pathway. SAM is converted to S‐adenosyl‐homocysteine (SAH) and finally to xanthine. SAH is a potent inhibitor of DNA methyltransferases, the reduction of which leads to increased DNA methylation and gene silencing in Arabidopsis. We report that theOsGDA1KO line exhibited a decrease in SAM, SAH and adenosine and an increase in rice genome methylation. The OsGDA1 protein phylogeny combined with mutational protein destabilization analysis suggested artificial selection for null mutants, which could affect genome methylation as in the KO line. Limited information on genes that may affect epigenetics indirectly requires deeper insights into such a role and effect of purine catabolism and related genetic networks. 2021-08 2024-12-19T12:53:43Z 2024-12-19T12:53:43Z Journal Article https://hdl.handle.net/10568/164306 en Open Access Wiley Gotarkar, Dhananjay; Longkumer, Toshisangba; Yamamoto, Naoki; Nanda, Amrit Kaur; Iglesias, Tamara; Li, Lin‐Feng; Miro, Berta; Blanco Gonzalez, Elisa; Montes Bayon, Maria; Olsen, Kenneth M.; Hsing, Yue‐Ie Caroline and Kohli, Ajay. 2021. A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome. Physiologia Plantarum, Volume 172 no. 4 p. 1853-1866 |
| spellingShingle | cell biology general medicine genetics physiology plant science Gotarkar, Dhananjay Longkumer, Toshisangba Yamamoto, Naoki Nanda, Amrit Kaur Iglesias, Tamara Li, Lin-Feng Miro, Berta Blanco Gonzalez, Elisa Montes Bayon, Maria Olsen, Kenneth M. Hsing, Yue-Ie Caroline Kohli, Ajay A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title | A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title_full | A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title_fullStr | A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title_full_unstemmed | A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title_short | A drought‐responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| title_sort | drought responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome |
| topic | cell biology general medicine genetics physiology plant science |
| url | https://hdl.handle.net/10568/164306 |
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