Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.)
Trichoderma biopriming enhances rice growth in drought-stressed soils by triggering various plant metabolic pathways related to antioxidative defense, secondary metabolites, and hormonal upregulation. In the present study, transcriptomic analysis of rice cultivar IR64 bioprimed with Trichoderma harz...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164297 |
| _version_ | 1855540833356349440 |
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| author | Bashyal, Bishnu Maya Parmar, Pooja Zaidi, Najam Waris Aggarwal, Rashmi |
| author_browse | Aggarwal, Rashmi Bashyal, Bishnu Maya Parmar, Pooja Zaidi, Najam Waris |
| author_facet | Bashyal, Bishnu Maya Parmar, Pooja Zaidi, Najam Waris Aggarwal, Rashmi |
| author_sort | Bashyal, Bishnu Maya |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Trichoderma biopriming enhances rice growth in drought-stressed soils by triggering various plant metabolic pathways related to antioxidative defense, secondary metabolites, and hormonal upregulation. In the present study, transcriptomic analysis of rice cultivar IR64 bioprimed with Trichoderma harzianum under drought stress was carried out in comparison with drought-stressed samples using next-generation sequencing techniques. Out of the 2,506 significant (p andlt; 0.05) differentially expressed genes (DEGs), 337 (15%) were exclusively expressed in drought-stressed plants, 382 (15%) were expressed in T. harzianum-treated drought-stressed plants, and 1,787 (70%) were commonly expressed. Furthermore, comparative analysis of upregulated and downregulated genes under stressed conditions showed that 1,053 genes (42%) were upregulated and 733 genes (29%) were downregulated in T. harzianum-treated drought-stressed rice plants. The genes exclusively expressed in T. harzianum-treated drought-stressed plants were mostly photosynthetic and antioxidative such as plastocyanin, small chain of Rubisco, PSI subunit Q, PSII subunit PSBY, osmoproteins, proline-rich protein, aquaporins, stress-enhanced proteins, and chaperonins. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis states that the most enriched pathways were metabolic (38%) followed by pathways involved in the synthesis of secondary metabolites (25%), carbon metabolism (6%), phenyl propanoid (7%), and glutathione metabolism (3%). Some of the genes were selected for validation using real-time PCR which showed consistent expression as RNA-Seq data. Furthermore, to establish host–T. harzianum interaction, transcriptome analysis of Trichoderma was also carried out. The Gene Ontology (GO) analysis of T. harzianum transcriptome suggested that the annotated genes are functionally related to carbohydrate binding module, glycoside hydrolase, GMC oxidoreductase, and trehalase and were mainly upregulated, playing an important role in establishing the mycelia colonization of rice roots and its growth. Overall, it can be concluded that T. harzianum biopriming delays drought stress in rice cultivars by a multitude of molecular programming. |
| format | Journal Article |
| id | CGSpace164297 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1642972024-12-19T14:13:40Z Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) Bashyal, Bishnu Maya Parmar, Pooja Zaidi, Najam Waris Aggarwal, Rashmi microbiology Trichoderma biopriming enhances rice growth in drought-stressed soils by triggering various plant metabolic pathways related to antioxidative defense, secondary metabolites, and hormonal upregulation. In the present study, transcriptomic analysis of rice cultivar IR64 bioprimed with Trichoderma harzianum under drought stress was carried out in comparison with drought-stressed samples using next-generation sequencing techniques. Out of the 2,506 significant (p andlt; 0.05) differentially expressed genes (DEGs), 337 (15%) were exclusively expressed in drought-stressed plants, 382 (15%) were expressed in T. harzianum-treated drought-stressed plants, and 1,787 (70%) were commonly expressed. Furthermore, comparative analysis of upregulated and downregulated genes under stressed conditions showed that 1,053 genes (42%) were upregulated and 733 genes (29%) were downregulated in T. harzianum-treated drought-stressed rice plants. The genes exclusively expressed in T. harzianum-treated drought-stressed plants were mostly photosynthetic and antioxidative such as plastocyanin, small chain of Rubisco, PSI subunit Q, PSII subunit PSBY, osmoproteins, proline-rich protein, aquaporins, stress-enhanced proteins, and chaperonins. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis states that the most enriched pathways were metabolic (38%) followed by pathways involved in the synthesis of secondary metabolites (25%), carbon metabolism (6%), phenyl propanoid (7%), and glutathione metabolism (3%). Some of the genes were selected for validation using real-time PCR which showed consistent expression as RNA-Seq data. Furthermore, to establish host–T. harzianum interaction, transcriptome analysis of Trichoderma was also carried out. The Gene Ontology (GO) analysis of T. harzianum transcriptome suggested that the annotated genes are functionally related to carbohydrate binding module, glycoside hydrolase, GMC oxidoreductase, and trehalase and were mainly upregulated, playing an important role in establishing the mycelia colonization of rice roots and its growth. Overall, it can be concluded that T. harzianum biopriming delays drought stress in rice cultivars by a multitude of molecular programming. 2021-04-13 2024-12-19T12:53:43Z 2024-12-19T12:53:43Z Journal Article https://hdl.handle.net/10568/164297 en Open Access Frontiers Media Bashyal, Bishnu Maya; Parmar, Pooja; Zaidi, Najam Waris and Aggarwal, Rashmi. 2021. Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.). Front. Microbiol., Volume 12 |
| spellingShingle | microbiology Bashyal, Bishnu Maya Parmar, Pooja Zaidi, Najam Waris Aggarwal, Rashmi Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title | Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title_full | Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title_fullStr | Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title_full_unstemmed | Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title_short | Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) |
| title_sort | molecular programming of drought challenged trichoderma harzianum bioprimed rice oryza sativa l |
| topic | microbiology |
| url | https://hdl.handle.net/10568/164297 |
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