Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.)
Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study wa...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2021
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| Acceso en línea: | https://hdl.handle.net/10568/164292 |
| _version_ | 1855535405284196352 |
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| author | Ali, Jauhar Nicolas, Katrina Leslie C. Akther, Shahana Torabi, Azerkhsh Ebadi, Ali Akbar Marfori-Nazarea, Corinne M. Mahender, Anumalla |
| author_browse | Akther, Shahana Ali, Jauhar Ebadi, Ali Akbar Mahender, Anumalla Marfori-Nazarea, Corinne M. Nicolas, Katrina Leslie C. Torabi, Azerkhsh |
| author_facet | Ali, Jauhar Nicolas, Katrina Leslie C. Akther, Shahana Torabi, Azerkhsh Ebadi, Ali Akbar Marfori-Nazarea, Corinne M. Mahender, Anumalla |
| author_sort | Ali, Jauhar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study was conducted to determine the effects of two improved anther culture media, Ali-1 (A1) and Ali-2 (A2), a modified N6 medium, to enhance the callus formation and plant regeneration of japonica, indica, and hybrids of indica and japonica cross. The current study demonstrated that genotype and media had a significant impact (p < 0.001) on both callus induction frequency and green plantlet regeneration efficiency. The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480. However, the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively. Furthermore, it substantially reduced the albino plantlet regeneration of the induced callus in two hybrids (CXY6 and Y2). Therefore, the improved anther culture medium A1 can produce doubled haploid rice plants for indica × japonica, which can be useful in different breeding programs that will enable the speedy development of rice varieties for resource-poor farmers. |
| format | Journal Article |
| id | CGSpace164292 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1642922024-12-22T05:44:59Z Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) Ali, Jauhar Nicolas, Katrina Leslie C. Akther, Shahana Torabi, Azerkhsh Ebadi, Ali Akbar Marfori-Nazarea, Corinne M. Mahender, Anumalla Anther culture technique is the most viable and efficient method of producing homozygous doubled haploid plants within a short period. However, the practical application of this technology in rice improvement is still limited by various factors that influence culture efficiency. The present study was conducted to determine the effects of two improved anther culture media, Ali-1 (A1) and Ali-2 (A2), a modified N6 medium, to enhance the callus formation and plant regeneration of japonica, indica, and hybrids of indica and japonica cross. The current study demonstrated that genotype and media had a significant impact (p < 0.001) on both callus induction frequency and green plantlet regeneration efficiency. The use of the A1 and A2 medium significantly enhanced callus induction frequency of japonica rice type, Nipponbare, and the hybrids of indica × japonica cross (CXY6, CXY24, and Y2) but not the indica rice type, NSIC Rc480. However, the A1 medium is found superior to the N6 medium as it significantly improved the green plantlet regeneration efficiency of CXY6, CXY24, and Y2 by almost 36%, 118%, and 277%, respectively. Furthermore, it substantially reduced the albino plantlet regeneration of the induced callus in two hybrids (CXY6 and Y2). Therefore, the improved anther culture medium A1 can produce doubled haploid rice plants for indica × japonica, which can be useful in different breeding programs that will enable the speedy development of rice varieties for resource-poor farmers. 2021-04-22 2024-12-19T12:53:43Z 2024-12-19T12:53:43Z Journal Article https://hdl.handle.net/10568/164292 en Open Access MDPI Ali, Jauhar; Nicolas, Katrina Leslie C.; Akther, Shahana; Torabi, Azerkhsh; Ebadi, Ali Akbar; Marfori-Nazarea, Corinne M. and Mahender, Anumalla. 2021. Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.). Plants, Volume 10 no. 5 p. 839 |
| spellingShingle | Ali, Jauhar Nicolas, Katrina Leslie C. Akther, Shahana Torabi, Azerkhsh Ebadi, Ali Akbar Marfori-Nazarea, Corinne M. Mahender, Anumalla Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title | Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title_full | Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title_fullStr | Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title_full_unstemmed | Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title_short | Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) |
| title_sort | improved anther culture media for enhanced callus formation and plant regeneration in rice oryza sativa l |
| url | https://hdl.handle.net/10568/164292 |
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