OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice
Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2021
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| Acceso en línea: | https://hdl.handle.net/10568/164189 |
| _version_ | 1855532341905063936 |
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| author | Zhu, Chen-li Xing, Bao Teng, Shouzhen Deng, Chen Shen, Zhen-yong Ai, Peng-fei Lu, Tiegang Zhang, Sheng-wei Zhang, Zhi-guo |
| author_browse | Ai, Peng-fei Deng, Chen Lu, Tiegang Shen, Zhen-yong Teng, Shouzhen Xing, Bao Zhang, Sheng-wei Zhang, Zhi-guo Zhu, Chen-li |
| author_facet | Zhu, Chen-li Xing, Bao Teng, Shouzhen Deng, Chen Shen, Zhen-yong Ai, Peng-fei Lu, Tiegang Zhang, Sheng-wei Zhang, Zhi-guo |
| author_sort | Zhu, Chen-li |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms to improve rice yield. We obtained a mutant rela (regulator of leaf angle) with reduced leaf angle in rice by EMS mutagenesis, and map-based cloning revealed that OsRELA encodes a protein of unknown function. Coincidentally, DENSE AND ERECT PANICLE 2 (DEP2) was reported in a previous study with the same gene locus. RNA-seq analysis revealed that OsRELA is involved in regulating the expression of ILI and Expansin family genes. Biochemical and genetic analyses revealed that OsRELA is able to interact with OsLIC, a negative regulator of BR signaling, through its conserved C-terminal domain, which is essential for OsRELA function in rice. The binding of OsRELA can activate the expression of downstream genes repressed by OsLIC, such as OsILI1, a positive regulator of leaf inclination in rice. Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC. |
| format | Journal Article |
| id | CGSpace164189 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1641892025-05-14T10:24:19Z OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice Zhu, Chen-li Xing, Bao Teng, Shouzhen Deng, Chen Shen, Zhen-yong Ai, Peng-fei Lu, Tiegang Zhang, Sheng-wei Zhang, Zhi-guo plant science Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms to improve rice yield. We obtained a mutant rela (regulator of leaf angle) with reduced leaf angle in rice by EMS mutagenesis, and map-based cloning revealed that OsRELA encodes a protein of unknown function. Coincidentally, DENSE AND ERECT PANICLE 2 (DEP2) was reported in a previous study with the same gene locus. RNA-seq analysis revealed that OsRELA is involved in regulating the expression of ILI and Expansin family genes. Biochemical and genetic analyses revealed that OsRELA is able to interact with OsLIC, a negative regulator of BR signaling, through its conserved C-terminal domain, which is essential for OsRELA function in rice. The binding of OsRELA can activate the expression of downstream genes repressed by OsLIC, such as OsILI1, a positive regulator of leaf inclination in rice. Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC. 2021-10-01 2024-12-19T12:53:35Z 2024-12-19T12:53:35Z Journal Article https://hdl.handle.net/10568/164189 en Open Access Frontiers Media Zhu, Chen-li; Xing, Bao; Teng, Shou-zhen; Deng, Chen; Shen, Zhen-yong; Ai, Peng-fei; Lu, Tie-gang; Zhang, Sheng-wei and Zhang, Zhi-guo. 2021. OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice. Front. Plant Sci., Volume 12 |
| spellingShingle | plant science Zhu, Chen-li Xing, Bao Teng, Shouzhen Deng, Chen Shen, Zhen-yong Ai, Peng-fei Lu, Tiegang Zhang, Sheng-wei Zhang, Zhi-guo OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title | OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title_full | OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title_fullStr | OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title_full_unstemmed | OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title_short | OsRELA regulates leaf inclination by repressing the transcriptional activity of OsLIC in rice |
| title_sort | osrela regulates leaf inclination by repressing the transcriptional activity of oslic in rice |
| topic | plant science |
| url | https://hdl.handle.net/10568/164189 |
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