Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar

Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with advanced bac...

Full description

Bibliographic Details
Main Authors: Carrillo, Maria Gay C., Martin, Federico, Variar, Mukund, Bhatt, J.C., Pérez-Quintero, Alvaro L., Leung, Hei, Leach, Jan E., Vera Cruz, Casiana M.
Format: Journal Article
Language:Inglés
Published: Springer 2021
Subjects:
Online Access:https://hdl.handle.net/10568/164169
_version_ 1855526572335824896
author Carrillo, Maria Gay C.
Martin, Federico
Variar, Mukund
Bhatt, J.C.
Pérez-Quintero, Alvaro L.
Leung, Hei
Leach, Jan E.
Vera Cruz, Casiana M.
author_browse Bhatt, J.C.
Carrillo, Maria Gay C.
Leach, Jan E.
Leung, Hei
Martin, Federico
Pérez-Quintero, Alvaro L.
Variar, Mukund
Vera Cruz, Casiana M.
author_facet Carrillo, Maria Gay C.
Martin, Federico
Variar, Mukund
Bhatt, J.C.
Pérez-Quintero, Alvaro L.
Leung, Hei
Leach, Jan E.
Vera Cruz, Casiana M.
author_sort Carrillo, Maria Gay C.
collection Repository of Agricultural Research Outputs (CGSpace)
description Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with advanced backcross populations, can accelerate the production of more resistant varieties. A candidate gene (CG) approach was used to accumulate different disease QTL from Moroberekan, a blast-resistant rice variety, into Vandana, a drought-tolerant variety. The advanced backcross progeny were evaluated for resistance to blast and tolerance to drought at five sites in India and the Philippines. Gene-based markers were designed to determine introgression of Moroberekan alleles for 11 CGs into the progeny. Six CGs, coding for chitinase, HSP90, oxalate oxidase, germin-like proteins, peroxidase and thaumatin-like protein, and 21 SSR markers were significantly associated with resistance to blast across screening sites. Multiple lines with different combinations, classes and numbers of CGs were associated with significant levels of race non-specific resistance to rice blast and sheath blight. Overall, the level of resistance effective in multiple locations was proportional to the number of CG alleles accumulated in advanced breeding lines. These disease resistant lines maintained tolerance to drought stress at the reproductive stage under blast disease pressure.
format Journal Article
id CGSpace164169
institution CGIAR Consortium
language Inglés
publishDate 2021
publishDateRange 2021
publishDateSort 2021
publisher Springer
publisherStr Springer
record_format dspace
spelling CGSpace1641692025-05-14T10:39:43Z Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar Carrillo, Maria Gay C. Martin, Federico Variar, Mukund Bhatt, J.C. Pérez-Quintero, Alvaro L. Leung, Hei Leach, Jan E. Vera Cruz, Casiana M. broad-spectrum resistance drought resistance rice blast rice cultivar Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with advanced backcross populations, can accelerate the production of more resistant varieties. A candidate gene (CG) approach was used to accumulate different disease QTL from Moroberekan, a blast-resistant rice variety, into Vandana, a drought-tolerant variety. The advanced backcross progeny were evaluated for resistance to blast and tolerance to drought at five sites in India and the Philippines. Gene-based markers were designed to determine introgression of Moroberekan alleles for 11 CGs into the progeny. Six CGs, coding for chitinase, HSP90, oxalate oxidase, germin-like proteins, peroxidase and thaumatin-like protein, and 21 SSR markers were significantly associated with resistance to blast across screening sites. Multiple lines with different combinations, classes and numbers of CGs were associated with significant levels of race non-specific resistance to rice blast and sheath blight. Overall, the level of resistance effective in multiple locations was proportional to the number of CG alleles accumulated in advanced breeding lines. These disease resistant lines maintained tolerance to drought stress at the reproductive stage under blast disease pressure. 2021-11-02 2024-12-19T12:53:32Z 2024-12-19T12:53:32Z Journal Article https://hdl.handle.net/10568/164169 en Open Access Springer Carrillo, Maria Gay C.; Martin, Federico; Variar, Mukund; Bhatt, J. C.; L. Perez-Quintero, Alvaro; Leung, Hei; Leach, Jan E. and Vera Cruz, Casiana M. 2021. Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar. Sci Rep, Volume 11, no. 1
spellingShingle broad-spectrum resistance
drought resistance
rice blast
rice cultivar
Carrillo, Maria Gay C.
Martin, Federico
Variar, Mukund
Bhatt, J.C.
Pérez-Quintero, Alvaro L.
Leung, Hei
Leach, Jan E.
Vera Cruz, Casiana M.
Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_full Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_fullStr Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_full_unstemmed Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_short Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_sort accumulating candidate genes for broad spectrum resistance to rice blast in a drought tolerant rice cultivar
topic broad-spectrum resistance
drought resistance
rice blast
rice cultivar
url https://hdl.handle.net/10568/164169
work_keys_str_mv AT carrillomariagayc accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT martinfederico accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT variarmukund accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT bhattjc accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT perezquinteroalvarol accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT leunghei accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT leachjane accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar
AT veracruzcasianam accumulatingcandidategenesforbroadspectrumresistancetoriceblastinadroughttolerantricecultivar