Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues
The translocation of photosynthate carbohydrates, such as sucrose, is critical for plant growth and crop yield. Previous studies have revealed that sugar transporters, plasmodesmata and sieve plates act as important controllers in sucrose loading into and unloading from phloem in the vascular system...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/164149 |
| _version_ | 1855542972181905408 |
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| author | Wang, Yanwei Sun, Jing Deng, Chen Teng, Shouzhen Chen, Guoxin Chen, Zhenhua Cui, Xuean Brutnell, Thomas P. Han, Xiao Zhang, Zhiguo Lu, Tiegang |
| author_browse | Brutnell, Thomas P. Chen, Guoxin Chen, Zhenhua Cui, Xuean Deng, Chen Han, Xiao Lu, Tiegang Sun, Jing Teng, Shouzhen Wang, Yanwei Zhang, Zhiguo |
| author_facet | Wang, Yanwei Sun, Jing Deng, Chen Teng, Shouzhen Chen, Guoxin Chen, Zhenhua Cui, Xuean Brutnell, Thomas P. Han, Xiao Zhang, Zhiguo Lu, Tiegang |
| author_sort | Wang, Yanwei |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The translocation of photosynthate carbohydrates, such as sucrose, is critical for plant growth and crop yield. Previous studies have revealed that sugar transporters, plasmodesmata and sieve plates act as important controllers in sucrose loading into and unloading from phloem in the vascular system. However, other pivotal steps for the regulation of sucrose movement remain largely elusive. In this study, characterization of two starch excesses in mesophyll (sem) mutants and dye and sucrose export assays were performed to provide insights into the regulatory networks that drive source–sink relations in rice. Map‐based cloning identified two allelic mutations in a gene encoding a GLUCAN SYNTHASE‐LIKE (GSL) protein, thus indicating a role for SEM1 in callose biosynthesis. Subcellular localization in rice showed that SEM1 localized to the plasma membrane. In situ expression analysis and GUS staining showed that SEM1 was mainly expressed in vascular phloem cells. Reduced sucrose transport was found in the sem1‐1/1‐2 mutant, which led to excessive starch accumulation in source leaves and inhibited photosynthesis. Paraffin section and transmission electron microscopy experiments revealed that less‐developed vascular cells (VCs) in sem1‐1/1‐2 potentially disturbed sugar movement. Moreover, dye and sugar trafficking experiments revealed that aberrant VC development was the main reason for the pleiotropic phenotype of sem1‐1/1‐2. In total, efficient sucrose loading into the phloem benefits from an optional number of VCs with a large vacuole that could act as a buffer holding tank for sucrose passing from the vascular bundle sheath. |
| format | Journal Article |
| id | CGSpace164149 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1641492024-12-22T05:45:03Z Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues Wang, Yanwei Sun, Jing Deng, Chen Teng, Shouzhen Chen, Guoxin Chen, Zhenhua Cui, Xuean Brutnell, Thomas P. Han, Xiao Zhang, Zhiguo Lu, Tiegang cell biology plant science genetics The translocation of photosynthate carbohydrates, such as sucrose, is critical for plant growth and crop yield. Previous studies have revealed that sugar transporters, plasmodesmata and sieve plates act as important controllers in sucrose loading into and unloading from phloem in the vascular system. However, other pivotal steps for the regulation of sucrose movement remain largely elusive. In this study, characterization of two starch excesses in mesophyll (sem) mutants and dye and sucrose export assays were performed to provide insights into the regulatory networks that drive source–sink relations in rice. Map‐based cloning identified two allelic mutations in a gene encoding a GLUCAN SYNTHASE‐LIKE (GSL) protein, thus indicating a role for SEM1 in callose biosynthesis. Subcellular localization in rice showed that SEM1 localized to the plasma membrane. In situ expression analysis and GUS staining showed that SEM1 was mainly expressed in vascular phloem cells. Reduced sucrose transport was found in the sem1‐1/1‐2 mutant, which led to excessive starch accumulation in source leaves and inhibited photosynthesis. Paraffin section and transmission electron microscopy experiments revealed that less‐developed vascular cells (VCs) in sem1‐1/1‐2 potentially disturbed sugar movement. Moreover, dye and sugar trafficking experiments revealed that aberrant VC development was the main reason for the pleiotropic phenotype of sem1‐1/1‐2. In total, efficient sucrose loading into the phloem benefits from an optional number of VCs with a large vacuole that could act as a buffer holding tank for sucrose passing from the vascular bundle sheath. 2022-02 2024-12-19T12:53:31Z 2024-12-19T12:53:31Z Journal Article https://hdl.handle.net/10568/164149 en Wiley Wang, Yanwei; Sun, Jing; Deng, Chen; Teng, Shouzhen; Chen, Guoxin; Chen, Zhenhua; Cui, Xuean; Brutnell, Thomas P.; Han, Xiao; Zhang, Zhiguo and Lu, Tiegang. 2022. Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues. The Plant Journal, Volume 109 no. 3 p. 523-540 |
| spellingShingle | cell biology plant science genetics Wang, Yanwei Sun, Jing Deng, Chen Teng, Shouzhen Chen, Guoxin Chen, Zhenhua Cui, Xuean Brutnell, Thomas P. Han, Xiao Zhang, Zhiguo Lu, Tiegang Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title | Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title_full | Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title_fullStr | Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title_full_unstemmed | Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title_short | Plasma membrane-localized SEM1 protein mediates sugar movement to sink rice tissues |
| title_sort | plasma membrane localized sem1 protein mediates sugar movement to sink rice tissues |
| topic | cell biology plant science genetics |
| url | https://hdl.handle.net/10568/164149 |
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