Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle
Mycoplasma mycoides subsp. mycoides SC is the pathogenic agent of contagious bovine pleuropneumonia (CBPP), the most important disease of cattle in Africa causing significant economic losses. The re-emergence of CBPP in Europe in the 1980s and 1990s illustrates that it is still a threat also to coun...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2011
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/16413 |
| _version_ | 1855534800103800832 |
|---|---|
| author | Schnee, C. Heller, M. Jores, Joerg Tomaso, H. Neubauer, N. |
| author_browse | Heller, M. Jores, Joerg Neubauer, N. Schnee, C. Tomaso, H. |
| author_facet | Schnee, C. Heller, M. Jores, Joerg Tomaso, H. Neubauer, N. |
| author_sort | Schnee, C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mycoplasma mycoides subsp. mycoides SC is the pathogenic agent of contagious bovine pleuropneumonia (CBPP), the most important disease of cattle in Africa causing significant economic losses. The re-emergence of CBPP in Europe in the 1980s and 1990s illustrates that it is still a threat also to countries that have successfully eradicated the disease in the past. Nowadays, probe-based real-time PCR techniques are among the most advanced tools for a reliable identification and a sensitive detection of many pathogens, but only few protocols have been published so far for CBPP diagnosis. Therefore we developed a novel TaqMan®-based real-time PCR assay comprising the amplification of two independent targets (MSC_0136 and MSC_1046) and an internal exogenous amplification control in a multiplex reaction and evaluated its diagnostic performance with clinical samples. |
| format | Journal Article |
| id | CGSpace16413 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace164132025-12-08T09:54:28Z Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle Schnee, C. Heller, M. Jores, Joerg Tomaso, H. Neubauer, N. diagnosis animal diseases cattle livestock Mycoplasma mycoides subsp. mycoides SC is the pathogenic agent of contagious bovine pleuropneumonia (CBPP), the most important disease of cattle in Africa causing significant economic losses. The re-emergence of CBPP in Europe in the 1980s and 1990s illustrates that it is still a threat also to countries that have successfully eradicated the disease in the past. Nowadays, probe-based real-time PCR techniques are among the most advanced tools for a reliable identification and a sensitive detection of many pathogens, but only few protocols have been published so far for CBPP diagnosis. Therefore we developed a novel TaqMan®-based real-time PCR assay comprising the amplification of two independent targets (MSC_0136 and MSC_1046) and an internal exogenous amplification control in a multiplex reaction and evaluated its diagnostic performance with clinical samples. 2011-08-12 2012-02-03T08:23:43Z 2012-02-03T08:23:43Z Journal Article https://hdl.handle.net/10568/16413 en Open Access Springer Schnee, C., Heller, M., Jores, J., Tomaso, H., & Neubauer, H. (2011). Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle. BMC Veterinary Research, 7(1), 47. https://doi.org/10.1186/1746-6148-7-47 |
| spellingShingle | diagnosis animal diseases cattle livestock Schnee, C. Heller, M. Jores, Joerg Tomaso, H. Neubauer, N. Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title | Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title_full | Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title_fullStr | Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title_full_unstemmed | Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title_short | Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle |
| title_sort | assessment of a novel multiplex real time pcr assay for the detection of the cbpp agent mycoplasma mycoides subsp mycoides sc through experimental infection in cattle |
| topic | diagnosis animal diseases cattle livestock |
| url | https://hdl.handle.net/10568/16413 |
| work_keys_str_mv | AT schneec assessmentofanovelmultiplexrealtimepcrassayforthedetectionofthecbppagentmycoplasmamycoidessubspmycoidesscthroughexperimentalinfectionincattle AT hellerm assessmentofanovelmultiplexrealtimepcrassayforthedetectionofthecbppagentmycoplasmamycoidessubspmycoidesscthroughexperimentalinfectionincattle AT joresjoerg assessmentofanovelmultiplexrealtimepcrassayforthedetectionofthecbppagentmycoplasmamycoidessubspmycoidesscthroughexperimentalinfectionincattle AT tomasoh assessmentofanovelmultiplexrealtimepcrassayforthedetectionofthecbppagentmycoplasmamycoidessubspmycoidesscthroughexperimentalinfectionincattle AT neubauern assessmentofanovelmultiplexrealtimepcrassayforthedetectionofthecbppagentmycoplasmamycoidessubspmycoidesscthroughexperimentalinfectionincattle |