Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives

We studied variation in adaptive traits and genetic association to understand the low P responses, including the symbiotic association of arbuscular mycorrhizal (AM) fungal colonization inOryzaspecies (O. sativa,O. nivara,andO. rufipogon). In the present experiment, we performed the phenotypic varia...

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Autores principales: Anandan, Annamalai, Nagireddy, Ranjitkumar, Sabarinathan, Selvaraj, Bhatta, Bishal Binaya, Mahender, Anumalla, Vinothkumar, Murugapandiyan, Parameswaran, Chidambaranathan, Panneerselvam, Periyasamy, Subudhi, Hatanath, Meher, Jitendriya, Bose, Lotan Kumar, Ali, Jauhar
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2022
Acceso en línea:https://hdl.handle.net/10568/164099
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author Anandan, Annamalai
Nagireddy, Ranjitkumar
Sabarinathan, Selvaraj
Bhatta, Bishal Binaya
Mahender, Anumalla
Vinothkumar, Murugapandiyan
Parameswaran, Chidambaranathan
Panneerselvam, Periyasamy
Subudhi, Hatanath
Meher, Jitendriya
Bose, Lotan Kumar
Ali, Jauhar
author_browse Ali, Jauhar
Anandan, Annamalai
Bhatta, Bishal Binaya
Bose, Lotan Kumar
Mahender, Anumalla
Meher, Jitendriya
Nagireddy, Ranjitkumar
Panneerselvam, Periyasamy
Parameswaran, Chidambaranathan
Sabarinathan, Selvaraj
Subudhi, Hatanath
Vinothkumar, Murugapandiyan
author_facet Anandan, Annamalai
Nagireddy, Ranjitkumar
Sabarinathan, Selvaraj
Bhatta, Bishal Binaya
Mahender, Anumalla
Vinothkumar, Murugapandiyan
Parameswaran, Chidambaranathan
Panneerselvam, Periyasamy
Subudhi, Hatanath
Meher, Jitendriya
Bose, Lotan Kumar
Ali, Jauhar
author_sort Anandan, Annamalai
collection Repository of Agricultural Research Outputs (CGSpace)
description We studied variation in adaptive traits and genetic association to understand the low P responses, including the symbiotic association of arbuscular mycorrhizal (AM) fungal colonization inOryzaspecies (O. sativa,O. nivara,andO. rufipogon). In the present experiment, we performed the phenotypic variability of the morphometric and geometric traits for P deficiency tolerance and conducted the association studies in GLM and MLM methods. A positive association between the geometric trait of the top-view area and root traits suggested the possibility of exploring a non-destructive approach in screening genotypes under low P. The AMOVA revealed a higher proportion of variation among the individuals as they belonged to different species ofOryzaand the NM value was 2.0, indicating possible gene flow between populations. A sub-cluster with superior-performing accessions had a higher proportion of landraces (42.85%), andO. rufipogon(33.3%) was differentiated by fourPup1-specific markers. Association mapping identified seven notable markers (RM259, RM297, RM30, RM6966, RM242, RM184, and PAP1) and six potential genotypes (IC459373, Chakhao Aumbi, AC100219, AC100062, Sekri, and Kumbhi Phou), which will be helpful in the marker-assisted breeding to improve rice for P-deprived condition. In addition, total root surface area becomes a single major trait that helps in P uptake under deficit P up to 33% than mycorrhizal colonization. Further, the phenotypic analysis of the morphometric and geometric trait variations and their interactions provides excellent potential for selecting donors for improving P-use efficiency. The identified potential candidate genes and markers offered new insights into our understanding of the molecular and physiological mechanisms driving PUE and improving grain yield under low-P conditions.
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spelling CGSpace1640992024-12-19T14:12:11Z Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives Anandan, Annamalai Nagireddy, Ranjitkumar Sabarinathan, Selvaraj Bhatta, Bishal Binaya Mahender, Anumalla Vinothkumar, Murugapandiyan Parameswaran, Chidambaranathan Panneerselvam, Periyasamy Subudhi, Hatanath Meher, Jitendriya Bose, Lotan Kumar Ali, Jauhar We studied variation in adaptive traits and genetic association to understand the low P responses, including the symbiotic association of arbuscular mycorrhizal (AM) fungal colonization inOryzaspecies (O. sativa,O. nivara,andO. rufipogon). In the present experiment, we performed the phenotypic variability of the morphometric and geometric traits for P deficiency tolerance and conducted the association studies in GLM and MLM methods. A positive association between the geometric trait of the top-view area and root traits suggested the possibility of exploring a non-destructive approach in screening genotypes under low P. The AMOVA revealed a higher proportion of variation among the individuals as they belonged to different species ofOryzaand the NM value was 2.0, indicating possible gene flow between populations. A sub-cluster with superior-performing accessions had a higher proportion of landraces (42.85%), andO. rufipogon(33.3%) was differentiated by fourPup1-specific markers. Association mapping identified seven notable markers (RM259, RM297, RM30, RM6966, RM242, RM184, and PAP1) and six potential genotypes (IC459373, Chakhao Aumbi, AC100219, AC100062, Sekri, and Kumbhi Phou), which will be helpful in the marker-assisted breeding to improve rice for P-deprived condition. In addition, total root surface area becomes a single major trait that helps in P uptake under deficit P up to 33% than mycorrhizal colonization. Further, the phenotypic analysis of the morphometric and geometric trait variations and their interactions provides excellent potential for selecting donors for improving P-use efficiency. The identified potential candidate genes and markers offered new insights into our understanding of the molecular and physiological mechanisms driving PUE and improving grain yield under low-P conditions. 2022-03-08 2024-12-19T12:53:28Z 2024-12-19T12:53:28Z Journal Article https://hdl.handle.net/10568/164099 en Open Access Springer Anandan, Annamalai; Nagireddy, Ranjitkumar; Sabarinathan, Selvaraj; Bhatta, Bishal Binaya; Mahender, Anumalla; Vinothkumar, Murugapandiyan; Parameswaran, Chidambaranathan; Panneerselvam, Periyasamy; Subudhi, Hatanath; Meher, Jitendriya; Bose, Lotan Kumar and Ali, Jauhar. 2022. Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives. Sci Rep, Volume 12, no. 1
spellingShingle Anandan, Annamalai
Nagireddy, Ranjitkumar
Sabarinathan, Selvaraj
Bhatta, Bishal Binaya
Mahender, Anumalla
Vinothkumar, Murugapandiyan
Parameswaran, Chidambaranathan
Panneerselvam, Periyasamy
Subudhi, Hatanath
Meher, Jitendriya
Bose, Lotan Kumar
Ali, Jauhar
Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title_full Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title_fullStr Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title_full_unstemmed Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title_short Multi-trait association study identifies loci associated with tolerance of low phosphorus in Oryza sativa and its wild relatives
title_sort multi trait association study identifies loci associated with tolerance of low phosphorus in oryza sativa and its wild relatives
url https://hdl.handle.net/10568/164099
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