Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize
Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice (Oryza sativa), the concentration of trans ‐zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this ho...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164077 |
| _version_ | 1855541303571382272 |
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| author | Cho, Lae-Hyeon Yoon, Jinmi Tun, Win Baek, Gibeom Peng, Xin Hong, Woo-Jong Mori, Izumi C. Hojo, Yuko Matsuura, Takakazu Kim, Sung-Ryul Kim, Sun-Tae Kwon, Soon-Wook Jung, Ki-Hong Jeon, Jong-Seong An, Gynheung |
| author_browse | An, Gynheung Baek, Gibeom Cho, Lae-Hyeon Hojo, Yuko Hong, Woo-Jong Jeon, Jong-Seong Jung, Ki-Hong Kim, Sun-Tae Kim, Sung-Ryul Kwon, Soon-Wook Matsuura, Takakazu Mori, Izumi C. Peng, Xin Tun, Win Yoon, Jinmi |
| author_facet | Cho, Lae-Hyeon Yoon, Jinmi Tun, Win Baek, Gibeom Peng, Xin Hong, Woo-Jong Mori, Izumi C. Hojo, Yuko Matsuura, Takakazu Kim, Sung-Ryul Kim, Sun-Tae Kwon, Soon-Wook Jung, Ki-Hong Jeon, Jong-Seong An, Gynheung |
| author_sort | Cho, Lae-Hyeon |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice (Oryza sativa), the concentration of trans ‐zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this hormone is involved in the regulation of the vegetative phase. To elucidate whether exogenous cytokinin application influences the length of the vegetative phase, we applied 6‐benzylaminopurine (BAP) to rice plants at various developmental stages. Our treatment delayed flowering time by 8–9 days when compared with mock‐treated rice plants, but only at the transition stage when the flowering signals were produced. Our observations also showed that flowering in the paddy field is delayed by thidiazuron, a stable chemical that mimics the effects of cytokinin. The transcript levels of florigen genes Heading date 3a (Hd3a) and Rice Flowering locus T1 (RFT1) were significantly reduced by the treatment, but the expression of Early heading date 1 (Ehd1), a gene found directly upstream of the florigen genes, was not altered. In maize (Zea mays), similarly, BAP treatment increased the vegetative phage by inhibiting the expression of ZCN8, an ortholog of Hd3a. We showed that cytokinin treatment induced the expression of two type‐A response regulators (OsRR1 and OsRR2) which interacted with Ehd1, a type‐B response regulator. We also observed that cytokinin did not affect flowering time in ehd1 knockout mutants. Our study indicates that cytokinin application increases the duration of the vegetative phase by delaying the expression of florigen genes in rice and maize by inhibiting Ehd1. |
| format | Journal Article |
| id | CGSpace164077 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1640772025-05-14T10:24:30Z Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize Cho, Lae-Hyeon Yoon, Jinmi Tun, Win Baek, Gibeom Peng, Xin Hong, Woo-Jong Mori, Izumi C. Hojo, Yuko Matsuura, Takakazu Kim, Sung-Ryul Kim, Sun-Tae Kwon, Soon-Wook Jung, Ki-Hong Jeon, Jong-Seong An, Gynheung cell biology plant science genetics Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice (Oryza sativa), the concentration of trans ‐zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this hormone is involved in the regulation of the vegetative phase. To elucidate whether exogenous cytokinin application influences the length of the vegetative phase, we applied 6‐benzylaminopurine (BAP) to rice plants at various developmental stages. Our treatment delayed flowering time by 8–9 days when compared with mock‐treated rice plants, but only at the transition stage when the flowering signals were produced. Our observations also showed that flowering in the paddy field is delayed by thidiazuron, a stable chemical that mimics the effects of cytokinin. The transcript levels of florigen genes Heading date 3a (Hd3a) and Rice Flowering locus T1 (RFT1) were significantly reduced by the treatment, but the expression of Early heading date 1 (Ehd1), a gene found directly upstream of the florigen genes, was not altered. In maize (Zea mays), similarly, BAP treatment increased the vegetative phage by inhibiting the expression of ZCN8, an ortholog of Hd3a. We showed that cytokinin treatment induced the expression of two type‐A response regulators (OsRR1 and OsRR2) which interacted with Ehd1, a type‐B response regulator. We also observed that cytokinin did not affect flowering time in ehd1 knockout mutants. Our study indicates that cytokinin application increases the duration of the vegetative phase by delaying the expression of florigen genes in rice and maize by inhibiting Ehd1. 2022-06 2024-12-19T12:53:27Z 2024-12-19T12:53:27Z Journal Article https://hdl.handle.net/10568/164077 en Wiley Cho, Lae‐Hyeon; Yoon, Jinmi; Tun, Win; Baek, Gibeom; Peng, Xin; Hong, Woo‐Jong; Mori, Izumi C.; Hojo, Yuko; Matsuura, Takakazu; Kim, Sung‐Ryul; Kim, Sun‐Tae; Kwon, Soon‐Wook; Jung, Ki‐Hong; Jeon, Jong‐Seong and An, Gynheung. 2022. Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize. The Plant Journal, Volume 110 no. 6 p. 1619-1635 |
| spellingShingle | cell biology plant science genetics Cho, Lae-Hyeon Yoon, Jinmi Tun, Win Baek, Gibeom Peng, Xin Hong, Woo-Jong Mori, Izumi C. Hojo, Yuko Matsuura, Takakazu Kim, Sung-Ryul Kim, Sun-Tae Kwon, Soon-Wook Jung, Ki-Hong Jeon, Jong-Seong An, Gynheung Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title | Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title_full | Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title_fullStr | Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title_full_unstemmed | Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title_short | Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| title_sort | cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize |
| topic | cell biology plant science genetics |
| url | https://hdl.handle.net/10568/164077 |
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