QTL mapping under salt stress in rice using a Kalarata–Azucena population

Salt stress is a major constraint across large rice production areas in Asia, because of the high sensitivity of modern rice varieties. To identify quantitative trait loci (QTL) associated with salt tolerance in rice, we developed an F2 population from a cross between the salt-tolerant landrace, Kal...

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Main Authors: de Ocampo, Marjorie P., Ho, Viet The, Thomson, Michael J., Mitsuya, Shiro, Yamauchi, Akira, Ismail, Abdelbagi M.
Format: Journal Article
Language:Inglés
Published: Springer 2022
Subjects:
Online Access:https://hdl.handle.net/10568/164068
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author de Ocampo, Marjorie P.
Ho, Viet The
Thomson, Michael J.
Mitsuya, Shiro
Yamauchi, Akira
Ismail, Abdelbagi M.
author_browse Ho, Viet The
Ismail, Abdelbagi M.
Mitsuya, Shiro
Thomson, Michael J.
Yamauchi, Akira
de Ocampo, Marjorie P.
author_facet de Ocampo, Marjorie P.
Ho, Viet The
Thomson, Michael J.
Mitsuya, Shiro
Yamauchi, Akira
Ismail, Abdelbagi M.
author_sort de Ocampo, Marjorie P.
collection Repository of Agricultural Research Outputs (CGSpace)
description Salt stress is a major constraint across large rice production areas in Asia, because of the high sensitivity of modern rice varieties. To identify quantitative trait loci (QTL) associated with salt tolerance in rice, we developed an F2 population from a cross between the salt-tolerant landrace, Kalarata, and the salt-sensitive parent, Azucena. F3 families from this population were screened and scored for salt tolerance using IRRI’s Standard evaluation system (SES). Growth, biomass, Na+ and K+ concentrations in leaf tissues, and chlorophyll concentration were determined. A genetic linkage map was constructed with 151 SSRs and InDel markers, which cover 1463 cM with an average distance of 9.69 cM between loci. A total of 13 QTL were identified using Composite Interval Mapping for 16 traits. Several novel QTL were identified in this study, the largest is for root sodium concentration (LOD = 11.0, R2 = 25.0) on chromosome 3, which also co-localize with a QTL for SES. Several QTL on the short arm of chromosome 1 coincide with the Saltol locus identified before. The novel QTL identified in this study constitute future targets for molecular breeding, to combine them with other QTL identified before, for higher tolerance and stable performance of rice varieties in salt affected soils.
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spelling CGSpace1640682024-12-19T14:12:08Z QTL mapping under salt stress in rice using a Kalarata–Azucena population de Ocampo, Marjorie P. Ho, Viet The Thomson, Michael J. Mitsuya, Shiro Yamauchi, Akira Ismail, Abdelbagi M. horticulture plant science genetics agronomy and crop science Salt stress is a major constraint across large rice production areas in Asia, because of the high sensitivity of modern rice varieties. To identify quantitative trait loci (QTL) associated with salt tolerance in rice, we developed an F2 population from a cross between the salt-tolerant landrace, Kalarata, and the salt-sensitive parent, Azucena. F3 families from this population were screened and scored for salt tolerance using IRRI’s Standard evaluation system (SES). Growth, biomass, Na+ and K+ concentrations in leaf tissues, and chlorophyll concentration were determined. A genetic linkage map was constructed with 151 SSRs and InDel markers, which cover 1463 cM with an average distance of 9.69 cM between loci. A total of 13 QTL were identified using Composite Interval Mapping for 16 traits. Several novel QTL were identified in this study, the largest is for root sodium concentration (LOD = 11.0, R2 = 25.0) on chromosome 3, which also co-localize with a QTL for SES. Several QTL on the short arm of chromosome 1 coincide with the Saltol locus identified before. The novel QTL identified in this study constitute future targets for molecular breeding, to combine them with other QTL identified before, for higher tolerance and stable performance of rice varieties in salt affected soils. 2022-06 2024-12-19T12:53:24Z 2024-12-19T12:53:24Z Journal Article https://hdl.handle.net/10568/164068 en Open Access Springer de Ocampo, Marjorie P.; Ho, Viet The; Thomson, Michael J.; Mitsuya, Shiro; Yamauchi, Akira and Ismail, Abdelbagi M. 2022. QTL mapping under salt stress in rice using a Kalarata–Azucena population. Euphytica, Volume 218, no. 6
spellingShingle horticulture
plant science
genetics
agronomy and crop science
de Ocampo, Marjorie P.
Ho, Viet The
Thomson, Michael J.
Mitsuya, Shiro
Yamauchi, Akira
Ismail, Abdelbagi M.
QTL mapping under salt stress in rice using a Kalarata–Azucena population
title QTL mapping under salt stress in rice using a Kalarata–Azucena population
title_full QTL mapping under salt stress in rice using a Kalarata–Azucena population
title_fullStr QTL mapping under salt stress in rice using a Kalarata–Azucena population
title_full_unstemmed QTL mapping under salt stress in rice using a Kalarata–Azucena population
title_short QTL mapping under salt stress in rice using a Kalarata–Azucena population
title_sort qtl mapping under salt stress in rice using a kalarata azucena population
topic horticulture
plant science
genetics
agronomy and crop science
url https://hdl.handle.net/10568/164068
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