Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand

The pathogenic fungus Colletotrichum gloeosporioides causes anthracnose disease, which is an important fungal disease affecting the production of numerous crops around the world. The presence of mycoviruses, however, may have an impact on the pathogenicity of the fungal host. Here, we describe a dou...

Descripción completa

Detalles Bibliográficos
Autores principales: Suharto, Aditya R., Jirakkakul, Jiraporn, Eusebio-Cope, Ana, Salaipeth, Lakha
Formato: Journal Article
Lenguaje:Inglés
Publicado: MDPI 2022
Materias:
Acceso en línea:https://hdl.handle.net/10568/164038
_version_ 1855521380068491264
author Suharto, Aditya R.
Jirakkakul, Jiraporn
Eusebio-Cope, Ana
Salaipeth, Lakha
author_browse Eusebio-Cope, Ana
Jirakkakul, Jiraporn
Salaipeth, Lakha
Suharto, Aditya R.
author_facet Suharto, Aditya R.
Jirakkakul, Jiraporn
Eusebio-Cope, Ana
Salaipeth, Lakha
author_sort Suharto, Aditya R.
collection Repository of Agricultural Research Outputs (CGSpace)
description The pathogenic fungus Colletotrichum gloeosporioides causes anthracnose disease, which is an important fungal disease affecting the production of numerous crops around the world. The presence of mycoviruses, however, may have an impact on the pathogenicity of the fungal host. Here, we describe a double-stranded RNA (dsRNA) mycovirus, which was isolated from a field strain of C. gloeosporioides, Ssa-44.1. The 2939 bp genome sequence comprises two open reading frames (ORFs) that encode for a putative protein and RNA-dependent RNA polymerase (RdRp). The Ssa-44.1 mycovirus is a member of the unclassified mycovirus family named Colletotrichum gloeosporioides RNA virus 1 strain Ssa-44.1 (CgRV1-Ssa-44.1), which has a phylogenetic similarity to Colletotrichum gleosporioides RNA virus 1 (CgRV1), which was isolated from citrus leaves in China. In C. gloeosporioides, CgRV1-Ssa-44.1 was shown to be linked to hypovirulence. CgRV1-Ssa-44.1 has a low spore transfer efficiency but can successfully spread horizontally to isogenic virus-free isolates. Furthermore, CgRV1-Ssa-44.1 had a strong biological control impact on C. gloeosporioides on mango plants. This study is the first to describe a hypovirulence-associated mycovirus infecting C. gloeosporioides, which has the potential to assist with anthracnose disease biological management.
format Journal Article
id CGSpace164038
institution CGIAR Consortium
language Inglés
publishDate 2022
publishDateRange 2022
publishDateSort 2022
publisher MDPI
publisherStr MDPI
record_format dspace
spelling CGSpace1640382025-12-08T10:29:22Z Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand Suharto, Aditya R. Jirakkakul, Jiraporn Eusebio-Cope, Ana Salaipeth, Lakha virology infectious diseases The pathogenic fungus Colletotrichum gloeosporioides causes anthracnose disease, which is an important fungal disease affecting the production of numerous crops around the world. The presence of mycoviruses, however, may have an impact on the pathogenicity of the fungal host. Here, we describe a double-stranded RNA (dsRNA) mycovirus, which was isolated from a field strain of C. gloeosporioides, Ssa-44.1. The 2939 bp genome sequence comprises two open reading frames (ORFs) that encode for a putative protein and RNA-dependent RNA polymerase (RdRp). The Ssa-44.1 mycovirus is a member of the unclassified mycovirus family named Colletotrichum gloeosporioides RNA virus 1 strain Ssa-44.1 (CgRV1-Ssa-44.1), which has a phylogenetic similarity to Colletotrichum gleosporioides RNA virus 1 (CgRV1), which was isolated from citrus leaves in China. In C. gloeosporioides, CgRV1-Ssa-44.1 was shown to be linked to hypovirulence. CgRV1-Ssa-44.1 has a low spore transfer efficiency but can successfully spread horizontally to isogenic virus-free isolates. Furthermore, CgRV1-Ssa-44.1 had a strong biological control impact on C. gloeosporioides on mango plants. This study is the first to describe a hypovirulence-associated mycovirus infecting C. gloeosporioides, which has the potential to assist with anthracnose disease biological management. 2022-08-30 2024-12-19T12:53:21Z 2024-12-19T12:53:21Z Journal Article https://hdl.handle.net/10568/164038 en Open Access MDPI Suharto, Aditya R.; Jirakkakul, Jiraporn; Eusebio-Cope, Ana and Salaipeth, Lakha. 2022. Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand. Viruses, Volume 14 no. 9 p. 1921
spellingShingle virology
infectious diseases
Suharto, Aditya R.
Jirakkakul, Jiraporn
Eusebio-Cope, Ana
Salaipeth, Lakha
Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title_full Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title_fullStr Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title_full_unstemmed Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title_short Hypovirulence of Colletotrichum gloesporioides associated with dsRNA mycovirus isolated from a mango orchard in Thailand
title_sort hypovirulence of colletotrichum gloesporioides associated with dsrna mycovirus isolated from a mango orchard in thailand
topic virology
infectious diseases
url https://hdl.handle.net/10568/164038
work_keys_str_mv AT suhartoadityar hypovirulenceofcolletotrichumgloesporioidesassociatedwithdsrnamycovirusisolatedfromamangoorchardinthailand
AT jirakkakuljiraporn hypovirulenceofcolletotrichumgloesporioidesassociatedwithdsrnamycovirusisolatedfromamangoorchardinthailand
AT eusebiocopeana hypovirulenceofcolletotrichumgloesporioidesassociatedwithdsrnamycovirusisolatedfromamangoorchardinthailand
AT salaipethlakha hypovirulenceofcolletotrichumgloesporioidesassociatedwithdsrnamycovirusisolatedfromamangoorchardinthailand