The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene
Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/163998 |
| _version_ | 1855517171640172544 |
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| author | Li, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K. Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming |
| author_browse | Chen, Guo-Kai Chen, Liang-Jwu Chiu, Tai-Yuan Hechanova, Sherry Lou Huang, Shou-Horng Jena, Kshirod K. Ke, Ting-Jyun Lai, Ming-Hsin Leu, Wei-Ming Li, Charng-Pei Meng, Menghsiao Shih, Hsien-Tzung Tsai, Kuan-Chieh Tsai, Zong-Yuan Wu, Dong-Hong |
| author_facet | Li, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K. Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming |
| author_sort | Li, Charng-Pei |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice. |
| format | Journal Article |
| id | CGSpace163998 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1639982025-12-08T10:29:22Z The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene Li, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K. Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming inorganic chemistry organic chemistry physical and theoretical chemistry computer science applications spectroscopy molecular biology general medicine catalysis Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice. 2023-01-16 2024-12-19T12:53:19Z 2024-12-19T12:53:19Z Journal Article https://hdl.handle.net/10568/163998 en Open Access MDPI Li, Charng-Pei; Wu, Dong-Hong; Huang, Shou-Horng; Meng, Menghsiao; Shih, Hsien-Tzung; Lai, Ming-Hsin; Chen, Liang-Jwu; Jena, Kshirod K.; Hechanova, Sherry Lou; Ke, Ting-Jyun; Chiu, Tai-Yuan; Tsai, Zong-Yuan; Chen, Guo-Kai; Tsai, Kuan-Chieh and Leu, Wei-Ming. 2023. The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene. IJMS, Volume 24 no. 2 p. 1798 |
| spellingShingle | inorganic chemistry organic chemistry physical and theoretical chemistry computer science applications spectroscopy molecular biology general medicine catalysis Li, Charng-Pei Wu, Dong-Hong Huang, Shou-Horng Meng, Menghsiao Shih, Hsien-Tzung Lai, Ming-Hsin Chen, Liang-Jwu Jena, Kshirod K. Hechanova, Sherry Lou Ke, Ting-Jyun Chiu, Tai-Yuan Tsai, Zong-Yuan Chen, Guo-Kai Tsai, Kuan-Chieh Leu, Wei-Ming The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title | The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title_full | The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title_fullStr | The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title_full_unstemmed | The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title_short | The Bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| title_sort | bph45 gene confers resistance against brown planthopper in rice by reducing the production of limonene |
| topic | inorganic chemistry organic chemistry physical and theoretical chemistry computer science applications spectroscopy molecular biology general medicine catalysis |
| url | https://hdl.handle.net/10568/163998 |
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