Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice
Magnesium (Mg) is an essential element for plant growth and development. Rice is an important food crop in the world, but there are few studies on the uptake and translocation of Mg2+ in rice. We used a multi-parent advanced generation inter-cross (MAGIC) population constructed using four parental l...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2023
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| Acceso en línea: | https://hdl.handle.net/10568/163980 |
| _version_ | 1855523317537046528 |
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| author | Zhi, Shuai Zou, Wenli Li, Jinyan Meng, Lijun Liu, Jindong Chen, Jingguang Ye, Guoyou |
| author_browse | Chen, Jingguang Li, Jinyan Liu, Jindong Meng, Lijun Ye, Guoyou Zhi, Shuai Zou, Wenli |
| author_facet | Zhi, Shuai Zou, Wenli Li, Jinyan Meng, Lijun Liu, Jindong Chen, Jingguang Ye, Guoyou |
| author_sort | Zhi, Shuai |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Magnesium (Mg) is an essential element for plant growth and development. Rice is an important food crop in the world, but there are few studies on the uptake and translocation of Mg2+ in rice. We used a multi-parent advanced generation inter-cross (MAGIC) population constructed using four parental lines and genotyped by a 55 K rice SNP array for association analysis to locate QTLs related to Mg2+ uptake and translocation in rice at the seedling stage. Four QTLs (qRMg1, qRMg2, qRMg7 and qRMg8) were detected for the root Mg2+ concentration, which explained 11.45-13.08% of the phenotypic variation. The Mg2+ transporter gene, OsMGT1, was within the region of qRMg1. Three QTLs (qSMg3, qSMg7 and qSMg10) were detected for the shoot Mg2+ concentration, which explained 4.30-5.46% of the phenotypic variation. Two QTLs (qTrMg3 and qTrMg8) were found to affect the translocation of Mg2+ from the roots to the shoots, and explained 10.91% and 9.63% of phenotypic variation. qSMg3 and qTrMg3 might be the same, since they are very close to each other on chromosome 3. Analysis of candidate genes in the region of qSMg3 and qTrMg3 through qRT-PCR, complementation assay in the yeast Mg2+ transport-defective mutant CM66, and sequence analysis of the parental lines suggested that LOC_Os03g04360 may play important roles in Mg2+ uptake, translocation and accumulation in rice. Overexpression of LOC_Os03g04360 can significantly increase the Mg2+ concentration in rice seedlings, especially under the condition of low Mg2+ supply. |
| format | Journal Article |
| id | CGSpace163980 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1639802025-12-08T10:29:22Z Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice Zhi, Shuai Zou, Wenli Li, Jinyan Meng, Lijun Liu, Jindong Chen, Jingguang Ye, Guoyou accumulation magnesium plant growth development rice food crop translocation genotyping phenotypic variation candidate genes Magnesium (Mg) is an essential element for plant growth and development. Rice is an important food crop in the world, but there are few studies on the uptake and translocation of Mg2+ in rice. We used a multi-parent advanced generation inter-cross (MAGIC) population constructed using four parental lines and genotyped by a 55 K rice SNP array for association analysis to locate QTLs related to Mg2+ uptake and translocation in rice at the seedling stage. Four QTLs (qRMg1, qRMg2, qRMg7 and qRMg8) were detected for the root Mg2+ concentration, which explained 11.45-13.08% of the phenotypic variation. The Mg2+ transporter gene, OsMGT1, was within the region of qRMg1. Three QTLs (qSMg3, qSMg7 and qSMg10) were detected for the shoot Mg2+ concentration, which explained 4.30-5.46% of the phenotypic variation. Two QTLs (qTrMg3 and qTrMg8) were found to affect the translocation of Mg2+ from the roots to the shoots, and explained 10.91% and 9.63% of phenotypic variation. qSMg3 and qTrMg3 might be the same, since they are very close to each other on chromosome 3. Analysis of candidate genes in the region of qSMg3 and qTrMg3 through qRT-PCR, complementation assay in the yeast Mg2+ transport-defective mutant CM66, and sequence analysis of the parental lines suggested that LOC_Os03g04360 may play important roles in Mg2+ uptake, translocation and accumulation in rice. Overexpression of LOC_Os03g04360 can significantly increase the Mg2+ concentration in rice seedlings, especially under the condition of low Mg2+ supply. 2023-02-23 2024-12-19T12:53:18Z 2024-12-19T12:53:18Z Journal Article https://hdl.handle.net/10568/163980 en Open Access Frontiers Media Zhi, Shuai; Zou, Wenli; Li, Jinyan; Meng, Lijun; Liu, Jindong; Chen, Jingguang and Ye, Guoyou. 2023. Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice. Front. Plant Sci., Volume 14 |
| spellingShingle | accumulation magnesium plant growth development rice food crop translocation genotyping phenotypic variation candidate genes Zhi, Shuai Zou, Wenli Li, Jinyan Meng, Lijun Liu, Jindong Chen, Jingguang Ye, Guoyou Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title | Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title_full | Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title_fullStr | Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title_full_unstemmed | Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title_short | Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice |
| title_sort | mapping qtls and gene validation studies for mg2 uptake and translocation using a magic population in rice |
| topic | accumulation magnesium plant growth development rice food crop translocation genotyping phenotypic variation candidate genes |
| url | https://hdl.handle.net/10568/163980 |
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