Genome-wide association analysis uncovers novel rice blast resistance alleles
<title></title> One of the most critical steps following genome-wide association studies (GWAS) is the identification and validation of candidate genes underlying genetic associations. Gene presence-absence and copy number variations can significantly hamper candidate gene discovery if high-quality...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Preprint |
| Lenguaje: | Inglés |
| Publicado: |
Research Square Platform LLC
2023
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| Acceso en línea: | https://hdl.handle.net/10568/163975 |
| Sumario: | <title></title> One of the most critical steps following genome-wide association studies (GWAS) is the identification and validation of candidate genes underlying genetic associations. Gene presence-absence and copy number variations can significantly hamper candidate gene discovery if high-quality genomic resources from specific crop cultivars are unavailable. This is particularly true for disease resistance genes, which are often located in highly dynamic genomic regions. Here, we perform a genome-wide association analysis of rice blast resistance in 500 genetically diverse rice accessions. To facilitate candidate gene identification, we produced <italic>de-novo</italic> genome assemblies of ten rice accessions that showed rice blast resistance associations. These assemblies facilitated the identification and validation of novel alleles of the rice blast resistance genes <italic>Ptr</italic> and <italic>Pia</italic>. Our work shows that GWAS in combination with whole-genome sequencing are powerful tools for gene cloning and uncovers an allelic series for the unusual <italic>Ptr</italic> rice blast resistance gene. |
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