Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice
Mesocotyl length (ML) is a crucial factor in determining the establishment and yield of rice planted through dry direct seeding, a practice that is increasingly popular in rice production worldwide. ML is determined by the endogenous and external environments, and inherits as a complex trait. To dat...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/163948 |
| _version_ | 1855538265429377024 |
|---|---|
| author | Meng, Yun Zhan, Junhui Liu, Hongyan Liu, Jindong Wang, Yamei Guo, Zhan He, Sang Nie, Lixiao Kohli, Ajay Ye, Guoyou |
| author_browse | Guo, Zhan He, Sang Kohli, Ajay Liu, Hongyan Liu, Jindong Meng, Yun Nie, Lixiao Wang, Yamei Ye, Guoyou Zhan, Junhui |
| author_facet | Meng, Yun Zhan, Junhui Liu, Hongyan Liu, Jindong Wang, Yamei Guo, Zhan He, Sang Nie, Lixiao Kohli, Ajay Ye, Guoyou |
| author_sort | Meng, Yun |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mesocotyl length (ML) is a crucial factor in determining the establishment and yield of rice planted through dry direct seeding, a practice that is increasingly popular in rice production worldwide. ML is determined by the endogenous and external environments, and inherits as a complex trait. To date, only a few genes have been cloned, and the mechanisms underlying mesocotyl elongation remain largely unknown. Here, through a genome‐wide association study using sequenced germplasm, we reveal that natural allelic variations in a mitochondrial transcription termination factor, OsML1, predominantly determined the natural variation of ML in rice. Natural variants in the coding regions of OsML1 resulted in five major haplotypes with a clear differentiation between subspecies and subpopulations in cultivated rice. The much‐reduced genetic diversity of cultivated rice compared to the common wild rice suggested that OsML1 underwent selection during domestication. Transgenic experiments and molecular analysis demonstrated that OsML1 contributes to ML by influencing cell elongation primarily determined by H2O2 homeostasis. Overexpression of OsML1 promoted mesocotyl elongation and thus improved the emergence rate under deep direct seeding. Taken together, our results suggested that OsML1 is a key positive regulator of ML, and is useful in developing varieties for deep direct seeding by conventional and transgenic approaches. |
| format | Journal Article |
| id | CGSpace163948 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1639482024-12-22T05:45:01Z Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice Meng, Yun Zhan, Junhui Liu, Hongyan Liu, Jindong Wang, Yamei Guo, Zhan He, Sang Nie, Lixiao Kohli, Ajay Ye, Guoyou direct sowing allelic heterogeneity haplotypes genetic variation domestication seedling emergence cell elongation Mesocotyl length (ML) is a crucial factor in determining the establishment and yield of rice planted through dry direct seeding, a practice that is increasingly popular in rice production worldwide. ML is determined by the endogenous and external environments, and inherits as a complex trait. To date, only a few genes have been cloned, and the mechanisms underlying mesocotyl elongation remain largely unknown. Here, through a genome‐wide association study using sequenced germplasm, we reveal that natural allelic variations in a mitochondrial transcription termination factor, OsML1, predominantly determined the natural variation of ML in rice. Natural variants in the coding regions of OsML1 resulted in five major haplotypes with a clear differentiation between subspecies and subpopulations in cultivated rice. The much‐reduced genetic diversity of cultivated rice compared to the common wild rice suggested that OsML1 underwent selection during domestication. Transgenic experiments and molecular analysis demonstrated that OsML1 contributes to ML by influencing cell elongation primarily determined by H2O2 homeostasis. Overexpression of OsML1 promoted mesocotyl elongation and thus improved the emergence rate under deep direct seeding. Taken together, our results suggested that OsML1 is a key positive regulator of ML, and is useful in developing varieties for deep direct seeding by conventional and transgenic approaches. 2023-08 2024-12-19T12:53:13Z 2024-12-19T12:53:13Z Journal Article https://hdl.handle.net/10568/163948 en Wiley Meng, Yun; Zhan, Junhui; Liu, Hongyan; Liu, Jindong; Wang, Yamei; Guo, Zhan; He, Sang; Nie, Lixiao; Kohli, Ajay and Ye, Guoyou. 2023. Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice. The Plant Journal, Volume 115 no. 4 p. 910-925 |
| spellingShingle | direct sowing allelic heterogeneity haplotypes genetic variation domestication seedling emergence cell elongation Meng, Yun Zhan, Junhui Liu, Hongyan Liu, Jindong Wang, Yamei Guo, Zhan He, Sang Nie, Lixiao Kohli, Ajay Ye, Guoyou Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title | Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title_full | Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title_fullStr | Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title_full_unstemmed | Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title_short | Natural variation of OsML1, a mitochondrial transcription termination factor, contributes to mesocotyl length variation in rice |
| title_sort | natural variation of osml1 a mitochondrial transcription termination factor contributes to mesocotyl length variation in rice |
| topic | direct sowing allelic heterogeneity haplotypes genetic variation domestication seedling emergence cell elongation |
| url | https://hdl.handle.net/10568/163948 |
| work_keys_str_mv | AT mengyun naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT zhanjunhui naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT liuhongyan naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT liujindong naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT wangyamei naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT guozhan naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT hesang naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT nielixiao naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT kohliajay naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice AT yeguoyou naturalvariationofosml1amitochondrialtranscriptionterminationfactorcontributestomesocotyllengthvariationinrice |