Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains
Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
eLife Sciences Publications, Ltd.
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/163930 |
| _version_ | 1855522597105565696 |
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| author | Schepler-Luu, Van Sciallano, Coline Stiebner, Melissa Ji, Chonghui Boulard, Gabriel Diallo, Amadou Auguy, Florence Char, Si Nian Arra, Yugander Schenstnyi, Kyrylo Buchholzer, Marcel Loo, Eliza PI Bilaro, Atugonza L Lihepanyama, David Mkuya, Mohammed Murori, Rosemary Oliva, Ricardo Cunnac, Sebastien Yang, Bing Szurek, Boris Frommer, Wolf B |
| author_browse | Arra, Yugander Auguy, Florence Bilaro, Atugonza L Boulard, Gabriel Buchholzer, Marcel Char, Si Nian Cunnac, Sebastien Diallo, Amadou Frommer, Wolf B Ji, Chonghui Lihepanyama, David Loo, Eliza PI Mkuya, Mohammed Murori, Rosemary Oliva, Ricardo Schenstnyi, Kyrylo Schepler-Luu, Van Sciallano, Coline Stiebner, Melissa Szurek, Boris Yang, Bing |
| author_facet | Schepler-Luu, Van Sciallano, Coline Stiebner, Melissa Ji, Chonghui Boulard, Gabriel Diallo, Amadou Auguy, Florence Char, Si Nian Arra, Yugander Schenstnyi, Kyrylo Buchholzer, Marcel Loo, Eliza PI Bilaro, Atugonza L Lihepanyama, David Mkuya, Mohammed Murori, Rosemary Oliva, Ricardo Cunnac, Sebastien Yang, Bing Szurek, Boris Frommer, Wolf B |
| author_sort | Schepler-Luu, Van |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and iTALes suppressing Xa1. Phylogenomics clustered these strains with Xoo from Southern-China. African rice varieties do not carry effective resistance. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. The strategy could help to protect global rice crops from BB pandemics. |
| format | Journal Article |
| id | CGSpace163930 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | eLife Sciences Publications, Ltd. |
| publisherStr | eLife Sciences Publications, Ltd. |
| record_format | dspace |
| spelling | CGSpace1639302026-01-06T12:03:45Z Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains Schepler-Luu, Van Sciallano, Coline Stiebner, Melissa Ji, Chonghui Boulard, Gabriel Diallo, Amadou Auguy, Florence Char, Si Nian Arra, Yugander Schenstnyi, Kyrylo Buchholzer, Marcel Loo, Eliza PI Bilaro, Atugonza L Lihepanyama, David Mkuya, Mohammed Murori, Rosemary Oliva, Ricardo Cunnac, Sebastien Yang, Bing Szurek, Boris Frommer, Wolf B rice livelihood asia africa americas tanzania Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and iTALes suppressing Xa1. Phylogenomics clustered these strains with Xoo from Southern-China. African rice varieties do not carry effective resistance. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. The strategy could help to protect global rice crops from BB pandemics. 2023-07-05 2024-12-19T12:53:12Z 2024-12-19T12:53:12Z Journal Article https://hdl.handle.net/10568/163930 en Open Access eLife Sciences Publications, Ltd. Ji, Chonghui; Boulard, Gabriel; Diallo, Amadou; Auguy, Florence; Char, Si Nian; Arra, Yugander; Schenstnyi, Kyrylo; Buchholzer, Marcel; Loo, Eliza PI; Bilaro, Atugonza L; Lihepanyama, David; Mkuya, Mohammed; Murori, Rosemary; Oliva, Ricardo; Cunnac, Sebastien; Yang, Bing; Szurek, Boris and Frommer, Wolf B. 2023. Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains. , Volume 12 |
| spellingShingle | rice livelihood asia africa americas tanzania Schepler-Luu, Van Sciallano, Coline Stiebner, Melissa Ji, Chonghui Boulard, Gabriel Diallo, Amadou Auguy, Florence Char, Si Nian Arra, Yugander Schenstnyi, Kyrylo Buchholzer, Marcel Loo, Eliza PI Bilaro, Atugonza L Lihepanyama, David Mkuya, Mohammed Murori, Rosemary Oliva, Ricardo Cunnac, Sebastien Yang, Bing Szurek, Boris Frommer, Wolf B Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_full | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_fullStr | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_full_unstemmed | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_short | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_sort | genome editing of an african elite rice variety confers resistance against endemic and emerging xanthomonas oryzae pv oryzae strains |
| topic | rice livelihood asia africa americas tanzania |
| url | https://hdl.handle.net/10568/163930 |
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