Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice
The present study evaluates marker assisted forward breeding (MAFB)-derived disease resistant introgression lines (ILs) which do not have the targeted resistance genes for bacterial blight (xa5 + xa13 + Xa21) and blast (Pi2 + Pi9 + Pi54). The ILs were derived in the background of two elite rice cult...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/163911 |
| _version_ | 1855522834777899008 |
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| author | Varanasi, Yasaswini Vishnu Priya Isetty, Subhakara Rao Revadi, Padmashree Balakrishnan, Divya Hajira, Shaik Prasad, Madamsetty Srinivasa Laha, Gouri Shankar Perraju, Puvvada Singh, Uma Maheshwar Singh, Vikas Kumar Kumar, Arvind Sundaram, Raman Meenakshi Badri, Jyothi |
| author_browse | Badri, Jyothi Balakrishnan, Divya Hajira, Shaik Isetty, Subhakara Rao Kumar, Arvind Laha, Gouri Shankar Perraju, Puvvada Prasad, Madamsetty Srinivasa Revadi, Padmashree Singh, Uma Maheshwar Singh, Vikas Kumar Sundaram, Raman Meenakshi Varanasi, Yasaswini Vishnu Priya |
| author_facet | Varanasi, Yasaswini Vishnu Priya Isetty, Subhakara Rao Revadi, Padmashree Balakrishnan, Divya Hajira, Shaik Prasad, Madamsetty Srinivasa Laha, Gouri Shankar Perraju, Puvvada Singh, Uma Maheshwar Singh, Vikas Kumar Kumar, Arvind Sundaram, Raman Meenakshi Badri, Jyothi |
| author_sort | Varanasi, Yasaswini Vishnu Priya |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The present study evaluates marker assisted forward breeding (MAFB)-derived disease resistant introgression lines (ILs) which do not have the targeted resistance genes for bacterial blight (xa5 + xa13 + Xa21) and blast (Pi2 + Pi9 + Pi54). The ILs were derived in the background of two elite rice cultivars, Krishna Hamsa [Recurrent Parent 1 (RP1)] and WGL 14 (RP2), involving multi-parent inter-crossing. Molecular characterization with gene specific markers for seven reported resistance genes each for bacterial blight (Xa33, Xa38, xa23, Xa4, xa8, Xa27 and Xa41) and blast (Pi1, Pi20, Pi38, Pib, Pitp, Pizt and Pi40) revealed the presence of xa8 and Xa38, in addition to the targeted xa5, xa13 and Xa21 for bacterial blight resistance and Pi1, Pi38, Pi40, Pi20, Pib and Pipt, in addition to the targeted Pi9 and Pi54, for blast resistance in various combinations. A maximum of nine resistance genes xa5 + Xa21 + Pi54 + xa8 + Pipt + Pi38 + Pi1 + Pi20 + Pib was observed in RP1-IL 19030 followed by eight genes xa5 + xa13 + Xa21 + xa8 + Pi9 + Pipt + Pi1 + Pi20 in two RP2-ILs, 19344 and 19347. ANOVA revealed the presence of significant variability for all the yield traits except “days to 50% flowering” (DFF). Box plots depicted the seasonal differences in the phenotypic expression of the yield traits. There was significant positive association of grain yield with days to flowering, tiller number and panicle number. Thousand grain weight is also significantly and positively correlated with grain yield. On the contrary, grain yield showed a significantly negative association with plant height. Multi-parent selective inter-crossing in the present study not only led to the development of high yielding disease resistant ILs but also enhanced recovery of the recurrent parent via selection for essential morphological features. More than 90.0% genetic similarity in the ILs based on SNP-based background selection demonstrated the success of multi-parent selective intercrossing in the development of disease resistant NILs. |
| format | Journal Article |
| id | CGSpace163911 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1639112025-12-08T10:29:22Z Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice Varanasi, Yasaswini Vishnu Priya Isetty, Subhakara Rao Revadi, Padmashree Balakrishnan, Divya Hajira, Shaik Prasad, Madamsetty Srinivasa Laha, Gouri Shankar Perraju, Puvvada Singh, Uma Maheshwar Singh, Vikas Kumar Kumar, Arvind Sundaram, Raman Meenakshi Badri, Jyothi blast background molecular characterization pitp plant height The present study evaluates marker assisted forward breeding (MAFB)-derived disease resistant introgression lines (ILs) which do not have the targeted resistance genes for bacterial blight (xa5 + xa13 + Xa21) and blast (Pi2 + Pi9 + Pi54). The ILs were derived in the background of two elite rice cultivars, Krishna Hamsa [Recurrent Parent 1 (RP1)] and WGL 14 (RP2), involving multi-parent inter-crossing. Molecular characterization with gene specific markers for seven reported resistance genes each for bacterial blight (Xa33, Xa38, xa23, Xa4, xa8, Xa27 and Xa41) and blast (Pi1, Pi20, Pi38, Pib, Pitp, Pizt and Pi40) revealed the presence of xa8 and Xa38, in addition to the targeted xa5, xa13 and Xa21 for bacterial blight resistance and Pi1, Pi38, Pi40, Pi20, Pib and Pipt, in addition to the targeted Pi9 and Pi54, for blast resistance in various combinations. A maximum of nine resistance genes xa5 + Xa21 + Pi54 + xa8 + Pipt + Pi38 + Pi1 + Pi20 + Pib was observed in RP1-IL 19030 followed by eight genes xa5 + xa13 + Xa21 + xa8 + Pi9 + Pipt + Pi1 + Pi20 in two RP2-ILs, 19344 and 19347. ANOVA revealed the presence of significant variability for all the yield traits except “days to 50% flowering” (DFF). Box plots depicted the seasonal differences in the phenotypic expression of the yield traits. There was significant positive association of grain yield with days to flowering, tiller number and panicle number. Thousand grain weight is also significantly and positively correlated with grain yield. On the contrary, grain yield showed a significantly negative association with plant height. Multi-parent selective inter-crossing in the present study not only led to the development of high yielding disease resistant ILs but also enhanced recovery of the recurrent parent via selection for essential morphological features. More than 90.0% genetic similarity in the ILs based on SNP-based background selection demonstrated the success of multi-parent selective intercrossing in the development of disease resistant NILs. 2023-08-21 2024-12-19T12:53:11Z 2024-12-19T12:53:11Z Journal Article https://hdl.handle.net/10568/163911 en Open Access MDPI Varanasi, Yasaswini Vishnu Priya; Isetty, Subhakara Rao; Revadi, Padmashree; Balakrishnan, Divya; Hajira, Shaik; Prasad, Madamsetty Srinivasa; Laha, Gouri Shankar; Perraju, Puvvada; Singh, Uma Maheshwar; Singh, Vikas Kumar; Kumar, Arvind; Sundaram, Raman Meenakshi and Badri, Jyothi. 2023. Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice. Plants, Volume 12 no. 16 p. 3012 |
| spellingShingle | blast background molecular characterization pitp plant height Varanasi, Yasaswini Vishnu Priya Isetty, Subhakara Rao Revadi, Padmashree Balakrishnan, Divya Hajira, Shaik Prasad, Madamsetty Srinivasa Laha, Gouri Shankar Perraju, Puvvada Singh, Uma Maheshwar Singh, Vikas Kumar Kumar, Arvind Sundaram, Raman Meenakshi Badri, Jyothi Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title | Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title_full | Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title_fullStr | Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title_full_unstemmed | Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title_short | Molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| title_sort | molecular and morphological characterization of introgression lines with resistance to bacterial leaf blight and blast in rice |
| topic | blast background molecular characterization pitp plant height |
| url | https://hdl.handle.net/10568/163911 |
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