Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses
Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-str...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2024
|
| Online Access: | https://hdl.handle.net/10568/163786 |
| _version_ | 1855530970990510080 |
|---|---|
| author | Urzo, Michael Louie R. Guinto, Timothy D. Eusebio-Cope, Ana Budot, Bernard O. Yanoria, Mary Jeanie T. Jonson, Gilda B. Arakawa, Masao Kondō, Hideki Suzuki, Nobuhiro |
| author_browse | Arakawa, Masao Budot, Bernard O. Eusebio-Cope, Ana Guinto, Timothy D. Jonson, Gilda B. Kondō, Hideki Suzuki, Nobuhiro Urzo, Michael Louie R. Yanoria, Mary Jeanie T. |
| author_facet | Urzo, Michael Louie R. Guinto, Timothy D. Eusebio-Cope, Ana Budot, Bernard O. Yanoria, Mary Jeanie T. Jonson, Gilda B. Arakawa, Masao Kondō, Hideki Suzuki, Nobuhiro |
| author_sort | Urzo, Michael Louie R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-stranded RNA (dsRNA) elements for a collection of 39 fungal strains of R. solani from the rice sheath blight samples from at least four major rice-growing areas in the Philippines and a reference isolate from the International Rice Research Institute, showing different colony phenotypes. Their dsRNA profiles suggested the presence of multiple viral infections among these Philippine R. solani populations. Using next-generation sequencing, the viral sequences of the three representative R. solani strains (Ilo-Rs-6, Tar-Rs-3, and Tar-Rs-5) from different rice-growing areas revealed the presence of at least 36 viruses or virus-like agents, with the Tar-Rs-3 strain harboring the largest number of viruses (at least 20 in total). These mycoviruses or their candidates are believed to have single-stranded RNA or dsRNA genomes and they belong to or are associated with the orders Martellivirales, Hepelivirales, Durnavirales, Cryppavirales, Ourlivirales, and Ghabrivirales based on their coding-complete RNA-dependent RNA polymerase sequences. The complete genome sequences of two novel RNA viruses belonging to the proposed family Phlegiviridae and family Mitoviridae were determined. |
| format | Journal Article |
| id | CGSpace163786 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1637862025-12-08T10:29:22Z Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses Urzo, Michael Louie R. Guinto, Timothy D. Eusebio-Cope, Ana Budot, Bernard O. Yanoria, Mary Jeanie T. Jonson, Gilda B. Arakawa, Masao Kondō, Hideki Suzuki, Nobuhiro Rice sheath blight, caused by the soil-borne fungus Rhizoctonia solani (teleomorph: Thanatephorus cucumeris, Basidiomycota), is one of the most devastating phytopathogenic fungal diseases and causes yield loss. Here, we report on a very high prevalence (100%) of potential virus-associated double-stranded RNA (dsRNA) elements for a collection of 39 fungal strains of R. solani from the rice sheath blight samples from at least four major rice-growing areas in the Philippines and a reference isolate from the International Rice Research Institute, showing different colony phenotypes. Their dsRNA profiles suggested the presence of multiple viral infections among these Philippine R. solani populations. Using next-generation sequencing, the viral sequences of the three representative R. solani strains (Ilo-Rs-6, Tar-Rs-3, and Tar-Rs-5) from different rice-growing areas revealed the presence of at least 36 viruses or virus-like agents, with the Tar-Rs-3 strain harboring the largest number of viruses (at least 20 in total). These mycoviruses or their candidates are believed to have single-stranded RNA or dsRNA genomes and they belong to or are associated with the orders Martellivirales, Hepelivirales, Durnavirales, Cryppavirales, Ourlivirales, and Ghabrivirales based on their coding-complete RNA-dependent RNA polymerase sequences. The complete genome sequences of two novel RNA viruses belonging to the proposed family Phlegiviridae and family Mitoviridae were determined. 2024-07-17 2024-12-19T12:53:01Z 2024-12-19T12:53:01Z Journal Article https://hdl.handle.net/10568/163786 en Open Access MDPI Urzo, Michael Louie R.; Guinto, Timothy D.; Eusebio-Cope, Ana; Budot, Bernard O.; Yanoria, Mary Jeanie T.; Jonson, Gilda B.; Arakawa, Masao; Kondo, Hideki and Suzuki, Nobuhiro. 2024. Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses. Viruses, Volume 16 no. 7 p. 1152 |
| spellingShingle | Urzo, Michael Louie R. Guinto, Timothy D. Eusebio-Cope, Ana Budot, Bernard O. Yanoria, Mary Jeanie T. Jonson, Gilda B. Arakawa, Masao Kondō, Hideki Suzuki, Nobuhiro Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title | Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title_full | Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title_fullStr | Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title_full_unstemmed | Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title_short | Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Rhizoctonia solani Revealed Multi-Infection by Diverse Groups of RNA Viruses |
| title_sort | metatranscriptomic sequencing of sheath blight associated isolates of rhizoctonia solani revealed multi infection by diverse groups of rna viruses |
| url | https://hdl.handle.net/10568/163786 |
| work_keys_str_mv | AT urzomichaellouier metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT guintotimothyd metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT eusebiocopeana metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT budotbernardo metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT yanoriamaryjeaniet metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT jonsongildab metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT arakawamasao metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT kondohideki metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses AT suzukinobuhiro metatranscriptomicsequencingofsheathblightassociatedisolatesofrhizoctoniasolanirevealedmultiinfectionbydiversegroupsofrnaviruses |